34 results about "Dna staining" patented technology

Methods for detecting a short tandem repeat polymorphism (STRP), such as fragile X syndrome, wherein PCR is used to amplify nucleic acid along the chromosome in the genomic DNA which includes all of the STRs of interest plus a substantial contiguous segment of the nucleic acid adjacent to the STRs. Single-stranded product is then obtained, and colorimetric-labeled oligonucleotides which target for (i) STRs and (ii) the contiguous DNA segment are hybridized with this single-stranded product which is then bound to a solid phase and separated from the remainder of the target material. The labeled oligonucleotide target material is recovered by treatment with base and then hybridized to a microarray having a plurality of spots containing suitable oligonucleotide probes complementary thereto. Following hybridization, colorimetric intensities of the hybridized labeled target material present at specific spots on the microarray are measured to obtain individual values which are compared with results from known control samples to accurately quantify the number of STRs in the region of interest of the DNA being analyzed.

A sperm chromatin structure detection kit and application thereof are provided. The kit includes an A reagent and a B reagent, with the A reagent including HCl, Tween-20 and a phosphate buffer solution, and the B reagent including acridine orange and water. The kit is used in human sperm chromatin DNA staining to determine the chromatin DNA fragmentation level and to calculate the ratio of spermswith DNA fragmentation, and can analyze the ratio of immature sperms through the degree of combination between chromatin and protein.

The invention relates to a method for dyeing DNA in cesium chloride density gradient mixed liquor through GelGreen dye, and belongs to the field of molecular biological technologies. The method includes the steps that step1, an environmental sample is collected, and the environmental total DNA is extracted; step2, environmental total DNA-cesium chloride mixed liquor is prepared, and the GelGreen dye is added into the environmental total DNA-cesium chloride mixed liquor; step3, ultrahigh-speed centrifugation is performed on the mixed liquor with the GelGreen dye to obtain the density gradient mixed liquor; step4, layering is performed on the density gradient mixed liquor under irradiation of a fluorescence transmission instrument with the excitation wavelength of 488 nm, and the density gradient mixed liquor in corresponding layers is extracted through an injector; step5, bacterium 16S rRNA gene real-time fluorescence quantified PCR analysis is performed on the DNA in the density gradient mixed liquor in all the layers. By dyeing the DNA in the cesium chloride density gradient mixed liquor based on the GelGreen dye, the detection sensitivity of the environmental DNA in the cesium chloride density gradient mixed liquor obtained after ultrahigh-speed density gradient centrifugation is greatly improved.

The invention discloses a method for rapidly identifying hybrids of gray feathers of Chinese goose species in the field of goose species detection. S1: collecting biological samples of gray feather individuals to be detected; S2: extracting genomic DNA from the biological samples of the individuals to be detected; S3: Using the extracted genomic DNA as a template, the designed and synthesized PCR primer combination is used as primers for conventional PCR reaction; S4: Use 1% agarose gel electrophoresis to separate PCR products, use DL2000 as DNAmarker, and Goldview I as DNA staining S5: Observe the amplified product under ultraviolet light or gel imaging system, if there is an amplified band at about 300bp, it is judged that the detected individual is a heterozygous individual, otherwise, if there is no amplified band at about 300bp, Then it is judged that the detected individual is a homozygous gray feather individual. Quickly and accurately identify gray feather heterozygous individuals, so as to eliminate them from the reserved breeding geese. Only one generation of detection is needed to make the breeding group achieve homozygous gray feather phenotype, improve breeding efficiency, and increase the purity of the population. Identify the method provided.

This Invention, a kind of DNA-based integrated circuit, with the DNA dyeing technology, the inlaying principle between anti-cancer medicine and DNA molecules, can change energy gap of DNA molecules to alter the conductivity of DNA molecules. Because that the diameter of DNA molecules is only about 2 nm, this kind of electronic element, which is not made with photolithography technologies, not only avoids the bottleneck of line width in production of photolithography-based ICs, but also limits the line width to 2 nm, much less than the minimum line width (0.13 mum or 130 nm) in semi-conductor production industry. It brings a practical approach to IC design beyond photolithography technologies, and ensures the development of ICs to micro-miniature predicted by the Moore Law.

The invention discloses a new standard method for measuring intracellular protein content, which is mainly achieved by preparing a standard curve and measuring samples to be tested, and adopts DNA fluorescent staining. DNA is the genetic material in cells, and different For cells, the DNA content is relatively stable, and using DNA to standardize the protein to be tested can overcome many shortcomings of protein standardization.