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Method for determining somatic cell count in milk

A technology for somatic cells and raw milk, which is applied in the fields of electrical digital data processing, special data processing applications, color/spectral characteristic measurement, etc. Achieving the effect of low price, simple and easy operation of equipment and instruments

Inactive Publication Date: 2006-06-21
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for measuring the number of somatic cells in milk are as follows: Microscopic examination is the standard method for measuring the number of somatic cells in milk, but the preparation of samples for counting and microscopic examination is time-consuming and laborious, and is not suitable for rapid on-site use and large batches; The preparation and microscopic examination of fluorescent microscope counting method take a lot of time and are not suitable for a large number of rapid detection applications; the California mastitis test (CMT) is based on experience, and the specific somatic cell count cannot be obtained, only a rough range can be divided ; High-speed somatic cell counters are extremely expensive and cannot be widely used

Method used

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  • Method for determining somatic cell count in milk
  • Method for determining somatic cell count in milk
  • Method for determining somatic cell count in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, a kind of method for measuring milk somatic cell number, carry out the following steps respectively:

[0036] 1) Take 2ml of fresh milk, add 4ml of diphenylamine reagent into it, and mix well; put it in a boiling water bath and heat it for 10-15 minutes under strong acidic conditions to make the raw milk appear colored,

[0037] 2) After cooling, inject the above-mentioned colored raw milk as a sample into a cuvette, use D65 as a standard light source, and make the distance between the sample and the standard light source 45cm. Under the premise of ensuring the above experimental conditions, in a special light box Capture the image with a digital camera to obtain the color information of the sample;

[0038] 3), and then use special image color processing software to calculate each color component R in the AdobeRGB color system 8 , G 8 , B 8 average value. At this time, R 8 Represents the average value of the red component, G 8 represents the mean va...

Embodiment 2

[0047] Embodiment 2, a method for measuring the number of milk somatic cells, respectively carry out the following steps:

[0048] Step 1), 2) are with embodiment 1;

[0049] 3), then calculate the average value of each color component R, G, B in its RGB system with special-purpose image color processing software. At this time, R represents the average value of the red component, G represents the average value of the green component, and B represents the average value of the blue component.

[0050] 4) The somatic cell content T per milliliter of sample can be calculated by any one of the following formulas:

[0051] T=-647.68R+155.74;

[0052] T=-952.73G+187.12;

[0053] T=-1129B+221.35;

[0054] The relationship between T and R is as follows Figure 4 As shown, from now on Figure 4 It is known that the correlation coefficient between R and T is 0.9589.

[0055] The relationship between T and G is as follows Figure 5 As shown, from now on Figure 5 It is known that...

Embodiment 3

[0058] Embodiment 3, a method for measuring the number of milk somatic cells, respectively carry out the following steps:

[0059] Step 1), 2) are with embodiment 1;

[0060] 3), and then use special image color processing software to calculate the tristimulus value of the XYZ color system of CIE: X represents the red primary color value, Y represents the green primary color value, and Z represents the blue primary color value;

[0061] 4) The somatic cell content per milliliter of sample can be calculated by any one of the following formulas:

[0062] T=-807.55X+170.66;

[0063] T=-852.99Y+178.16;

[0064] T=-1010Z+216.72;

[0065] The relationship between T and X is as follows Figure 7 As shown, from now on Figure 7 It is known that the correlation coefficient between X and T is 0.9782.

[0066] The relationship between T and Y is as follows Figure 8 As shown, from now on Figure 8 It is known that the correlation coefficient between Y and T is 0.986.

[0067] Th...

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Abstract

The invention discloses a gauging method of milk cell quantity, which comprises the following steps: (1) adding the color-developing agent of random commissioning ratio in the crude milk; displaying color of crude milk in the boiling-water in the strong acidic condition; preparing the color-developing agent based on DNA dyeing agent; (2) placing the crude milk with color in the colorimetric device; adapting D65 as standard light source to make the distance between sample and standard light source as 45 cm; obtaining the color information of sample through image intaking equipment; 3) counting the color information to gain the average value of corresponding color system component; 4) calculating through the algorithm formula.

Description

technical field [0001] The invention relates to a method for measuring the number of somatic cells in milk. Background technique [0002] Dairy products are one of the most nutritious foods and are developing very fast in our country; raw milk is the basis for processing all dairy products. Among the quality indicators of raw milk, somatic cell count is an important quality and hygiene indicator. The number of somatic cells refers to the total number of cells per milliliter of milk. 98% to 99% of somatic cells are white blood cells (ie, macrophages, neutrophils and lymphocytes), and the other 1% to 2% are exfoliated breast tissue. Epithelial Cells. Therefore, the main composition of somatic cells in raw milk is leukocytes, and its quantity in raw milk is directly related to the quality and hygienic state of raw milk; because, only under the conditions of bacterial infection or other stimuli, the animal itself will produce emergency, produce A large number of white blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/27G06F19/00
Inventor 叶兴乾程文健刘东红陈健初童军锋
Owner ZHEJIANG UNIV
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