DNA ploidy staining solution as well as preparation method and staining method thereof

Abstract

The invention relates to the technical field of biology, in particular to a DNA ploidy staining solution as well as a preparation method and a staining method thereof, and the prepared DNA staining solution can solve the problems that the background is easy to co-stain, the staining time is relatively long and slices are easy to fade in flaking, so that a reliable histochemical staining technologyis provided for tumor cell nucleus DNA content image determination. Alcohol with the concentration of 95% is used for fixing cell nucleuses, the situation that in the dyeing hydrolysis process of unfixed cell nucleuses, DNA can form small fragments and dissociate out of the cell nucleuses, the DNA measurement accuracy is affected is avoided, aldehyde groups generated before hydrolysis can be sealed through fixation, and false positive products are reduced. Meanwhile, the BS stationary liquid can be used for changing the space structure of DNA molecules, so that the sensitivity to acid hydrolysis is influenced, and the situation that chromatin coloring is relatively light due to improper selection of the stationary liquid or too long tissue fixing time and relatively large difference in strength of dyeing reaction is avoided. Through gradient alcohol dehydration, the DNA staining result is clear, and the background is cleaner.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DNA ploid staining solution, a preparation method and a staining method. Background technique [0002] Analyzing the cell cycle and DNA ploidy of cells has been widely used clinically, providing an important basis for tumor diagnosis, curative effect evaluation and prognosis prediction. It is also more and more used in scientific research for cell dynamics, cell regulation death observation etc. DNA ploidy analysis combined with clinicopathological morphological diagnosis can be used for early diagnosis, follow-up and early treatment of some malignant tumors, so as to improve the cure rate and survival rate of tumors. Especially for the analysis of cell extracts, body fluids, glandular secretions, and exfoliated cells of tissues, the significance is very important. DNA ploidy analysis technology is of great value in the early diagnosis and prognosis of bladder cancer, prostate ca...

AI Technical Summary

Problems solved by technology

[0004] The DNA-Feulgen staining method is still one of the main staining methods for quantitative determination of DNA, but the key to traditional Feulgen staining is acid hydrolysis, insufficient or excessive hydrolysis, which can affect the staining effect, and the traditional Feulgen staining solution has low staining specificity , the background is easy to co-stain, the configuration of the dye solution is complicated, the reaction conditions must be strict, the staining time is long, and the sections are easy to fade, etc.