DNA staining and eosin staining comparative analysis method and system

Abstract

The invention discloses a DNA staining and eosin staining comparative analysis method and system.The comparative analysis method comprises the steps of placing a DNA staining slide in an optical scanner through a slide loader, generating a digital image through a controller, and transmitting the image to a server; segmenting the digital picture through a first artificial intelligence algorithm, and classifying cell nucleuses in the digital picture into epithelial cell nucleuses, neutrophil nucleuses and lymphocyte nucleuses; recognizing epithelial cell nucleuses of which the DNA indexes are greater than a set threshold value, and storing the positions of the epithelial cell nucleuses in the whole slide; carrying out DNA eosin staining on the slide again, scanning the slide again, and showing the cytoplasm of the cell nucleus through a staining agent; cutting the epithelial cell nucleus of which the DNA index is greater than a set threshold value from the whole picture; and performing redetermination verification on the cut epithelial cell nucleus through a second artificial intelligence algorithm in the server to determine whether the epithelial cell nucleus is the epithelial cell nucleus. According to the method, the analysis accuracy can be improved.

Description

Technical field [0001] The present invention belongs to the technical field of artificial intelligence, to a staining method, particularly to a method and system for Comparison DNA and eosin staining. Background technique [0002] At present the traditional diagnosis is made after the adoption of stained slides, one by one vision viewed by laboratory experts through a microscope. Since the film is relatively large field of view, the large number of nuclei, which requires experts a long time to determine a good diagnostic pathology slides, and is likely to cause visual fatigue due to the possible omission of cancer cells. [0003] In addition, since each has its own laboratory physician subjective judgment easily lead to a different film with a medical examination, there will be no results. [0004] In view of this, now an urgent need to design a new way of comparative analysis of stained slides, so as to at least partially overcome the above drawbacks of the prior embodiment Comp...

AI Technical Summary

Problems solved by technology

Due to the relatively large field of view of the film and the large number of nuclei, it takes a long time for experts to confirm the diagnosis of a pathological slide, and it is very likely that possible cancer cells may be missed due to visual fatigue.

[0003] In addition, because each examiner has his own subjective judgment, it is easy to cause the same film to be checked by different doctors, and there will be different results.

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