Safe and environment-friendly DNA (Deoxyribose Nucleic Acid) silver staining method for polyacrylamide gel

Abstract

The invention relates to a method for dyeing DNA (Deoxyribose Nucleic Acid) on polyacrylamide gel. The method is a safe and environment-friendly method for dyeing acidic silver ion solution. The method sequentially comprises the steps of fixing, primary washing, impregnating, secondary washing, developing, and developing reaction ending. In addition, the invention further relates to a method for detecting the DNA on the polyacrylamide gel, and a kit for the methods.

Description

technical field [0001] The invention belongs to the technical field of biological detection. Specifically, the invention relates to a method for staining and developing DNA with reducing sugar on polyacrylamide gel. The method is safe and environmentally friendly, has simple steps, short operation time and high sensitivity. In addition, the present invention also relates to methods for detecting DNA and kits for these methods, etc. Background of the invention [0002] Currently, separating DNA by electrophoresis on a polyacrylamide gel and developing (visualizing) the DNA bands has become one of the most commonly used methods in biological detection. Among them, fluorescent dyes, visible organic dyes and silver visible organic dyes such as methylene blue can be used to visualize DNA strips on polyacrylamide gels. [1] , bright cresyl blue [2] ,purple crystal [3] , and Nile Blue [4，5] etc., its sensitivity is low and the operation is time-consuming. Dyeing etc. Fluoresce...

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Problems solved by technology

One type, known as silver ammonia staining, uses a silver-ammonia solution to impregnate the gel and develops the color with an acidic solution containing formaldehyde, but is less sensitive and time-consuming [15] ; the other is stained with an acidic silver ion solution, in which the gel is impregnated in silver nitrate with a slightly acidic pH, and then reduced

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