7 results about "Acridine orange" patented technology

The application discloses a synthesis method and application of an acridine orange-polythiophene fluorescent probe, and the structural formula of the fluorescent probe is shown in the following formula: wherein m is an integer of 5-30, and n=100-m.The main chain of the fluorescent probe is a cationic polythiophene, which can enter a cell nucleus and be combined with nucleic acid through electrostatic action; the side chain modified acridine orange enhances the fluorescence intensity of the polymer and is combined with nucleic acid by embedding; and the side chain modified quaternary ammonium salt can reduce cytotoxicity.The fluorescent probe has the advantages of good distinguishability, good chemical stability, small sample consumption and the like, and is a novel fluorescent probe for high-selectivity recognition and inhibition of ecDNA replication in cancer cells in a physiological system.

This invention relates to the field of fluorescent probe technology, specifically to a triphenylamine amphiphilic fluorescent probe, its preparation method, and its applications. The triphenylamine amphiphilic fluorescent probe is synthesized in three steps: bromination, coupling, and condensation to construct a D-π-A molecular structure, producing far-red light emission at 650-660 nm and exhibiting aggregation-induced emission (AIE) properties. The pyridine group endows it with hydrophilicity and cationic characteristics, while the triphenylamine group endows it with lipophilicity and AIE properties. It can specifically bind to bacterial surface anions, enabling wash-free, high-contrast real-time imaging, and can also disrupt bacterial biofilms to exert antibacterial effects. The probe has a MIC as low as 1.5625 μM against bacteria such as Staphylococcus aureus and Escherichia coli, can be co-stained with acridine orange for verification, is compatible with conventional detection equipment, and achieves integrated bacterial diagnosis and treatment, solving the problems of fluorescence quenching and low emission peaks in traditional probes.

The invention discloses a three-emission-ratio sensing material and a preparation method and application thereof, and relates to the field of sensing materials.The preparation method comprises the following steps that S1, a metal organic framework material is dispersed in an organic solvent, a functional monomer and 4, 4 '-dimethyl benzophenone are added, and a mixed solution I is obtained; s2, adding an ATMP solution, an allyl rhodamine B solution, a cross-linking agent and an initiator into the mixed solution I while stirring to obtain a mixed solution II; s3, under the protection of inert gas, heating and refluxing the mixed solution II to initiate a polymerization reaction; and S4, separating a product after the polymerization reaction, eluting 4, 4 '-dimethyl benzophenone in the product, and drying to obtain the three-emission-ratio sensing material. Specific recognition and stability of a surface molecular imprinting technology are combined with signal output capability and high sensitivity of a double-fluorophore ratio sensing analysis technology, so that the purpose of visual quantitative detection of acridine orange is achieved, and the method has good reliability in the aspect of visual detection of acridine orange.

The invention discloses a kit and a method for combined detection of sperm cell active oxygen and DNA fragment index, and belongs to the technical field of reproductive biology and clinical diagnosis. The kit comprises reactive oxygen dye, acridine orange dye, acidizing fluid and buffer solution. According to the invention, a specific fluorescent dye is combined with a sperm DNA fragment detection method to obtain a method capable of jointly detecting the sperm cell DNA fragment index and the active oxygen, the detection method can be used for simultaneously detecting in the same tube buffer system, and signals among detected items are not influenced. By adopting the detection method disclosed by the invention, the sperm cells with high active oxygen, high DFI and extremely poor quality (ROS + DFI +) and low active oxygen, low DFI and extremely good quality (ROS-DFI-) can be obviously detected, and clinical research and screening of sperm cells with higher quality and distinguishing of poorer sperm cells can be facilitated.

The application discloses a method for packaging fluorophores based on tris-dolene nanofilm. First, a nanofilm with tris-dolene as a core segment is prepared through gas-liquid interface assembly and condensation reaction, and is carried on a quartz glass. In a wet state, the pore structure of the nanofilm can be maintained without collapsing through freeze-drying treatment. Then, a fluorophore solution is added dropwise on the nanofilm. After the solvent is naturally evaporated, the fluorophore is uniformly dispersed in the nanofilm, spatial and electronic isolation of the fluorophore is realized, and a flexible and uniform fluorescent nanofilm is obtained. The fluorophore packaged by the method includes coumarin (such as 7-(diethylamino) coumarin-3-carbonitrile and coumarin 6), acridine (such as acridine orange), xanthene (such as rhodamine 6G and rhodamine B) and quinonimine (such as Nile red), etc. In addition, the optical properties and sensing behavior of the obtained fluorescent nanofilm are consistent with the optical properties and sensing behavior of the fluorophore in a solution state. Therefore, the application has universality and fidelity.

The invention provides a fungus multi-fluorescent dyeing preparation and a preparation method and application thereof.The dyeing preparation comprises a solution A and a solution B. The solution A is mainly prepared from, by mass, 0.6-1 part of CYP51 fluorescent dye, 5-10 parts of fluorescent whitening agent, 3-4 parts of potassium hydroxide, 0.2-1 part of dimethyl sulfoxide and a proper amount of glycerin; the liquid B is mainly prepared from the following components in parts by mass: 0.05 to 0.2 part of ethidium bromide, 0.05 to 0.2 part of acridine orange, 0.5 to 2 parts of calcium fluorescent white, 7 to 10 parts of propidium iodide, 0.3 to 1 part of dimethyl sulfoxide and a proper amount of glycerol. According to the fluorescent staining preparation, the staining accuracy is improved, and dead bacteria and drug-resistant bacteria in fungi can be better stained.

The invention relates to the field of biological agents, particularly discloses an immune color developing reagent for detecting malignant tumors in urine and application of the immune color developing reagent, and is used for solving the problems that in bladder cancer urine exfoliated cell detection, an acridine orange single staining color developing image is poor in bladder tumor specific epitope recognition, heavy in background interference and high in spectrum overlapping and manual interpretation subjectivity. The color developing reagent comprises a reagent A, a reagent B, a reagent C and a reagent D. The reagent A is provided with an antibody complex and acridine orange aiming at tumor cell target antigens of bladder parts in urine shedding, and the reagent B comprises a fluorescently-labeled secondary antibody composition and an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent A, and the reagent D is provided with an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent D. In the application, AI imaging analysis is adopted, and layered identification labels and acridine orange dyeing negative and positive labels are calibrated; through multi-target immune color development, two sets of color separation amplification schemes and AI imaging analysis, the bladder tumor specific epitope recognition capability, the image quality and the image analysis accuracy of urine cast-off cell detection are improved.