Method for measuring carbendazim in water by using supramolecular complex fluorescence probe

A fluorescent probe, carbendazim technology, applied in the field of analytical chemistry, to achieve rapid on-site detection and rapid method effects

Active Publication Date: 2015-08-05
中知在线股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no literature report on the detection of the pesticide carbendazim by using cu

Method used

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  • Method for measuring carbendazim in water by using supramolecular complex fluorescence probe
  • Method for measuring carbendazim in water by using supramolecular complex fluorescence probe
  • Method for measuring carbendazim in water by using supramolecular complex fluorescence probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of Probe S Solution

[0029] Reagents: Acridine orange (analytical pure) was purchased from Shanghai Aladdin Reagent Company, and melon ring was obtained from the Key Laboratory of Supramolecular Macrocyclic Chemistry of Guizhou University (purity ≥ 98%).

[0030] Method: Accurately weigh 15.1 mg of eight-membered melon rings and 3.70 mg of acridine orange and mix them with ultrapure water to prepare 100 mL, the molar concentration ratio is 1:1, and the molar concentration is 1×10 -4 mol / L probe S solution.

Embodiment 2

[0031] Embodiment two: the establishment of detection method

[0032] Reagents: Carbendazim (analytical grade) was purchased from Shanghai Aladdin Reagent Company.

[0033] Method: Take 11 10 mL volumetric flasks and add 1×10 -4 After 1.0 mL of mol / L fluorescent probe S solution, add 0, 6.0, 12.0, 18.0, 24.0, 30.0, 36.0, 42.0, 48.0, 54.0, 60.0 microliters 1.0×10 -3 mol / L carbendazim standard solution, after adding 0.1mol / L HCl aqueous solution to adjust the pH=2.0, distilled water to the scale, shake well, after 10 minutes at room temperature, fixed excitation wavelength 495nm, slit 5nm, voltage 550v Perform fluorescence emission spectrometry. Each group of experiments was measured in parallel three times, with the concentration of carbendazim as the abscissa, and the average value of the fluorescence emission spectrum intensity at 529 nm in the three experiments as the ordinate to draw a standard curve. According to the experimental results, the concentration linear range ...

Embodiment 3

[0035] Example 3: Interference experiment of interfering ions on the system

[0036] Reagent: metal ion perchlorate (MClO 4 · n h 2 O, analytically pure) was purchased from Shanghai Aladdin Reagent Company.

[0037] Method: take common metal perchlorate, Fe 3+ , Mn 2+ , Al 3+ , Na + , Cu 2+ , Ca 2+ , Mg 2+ , Li + , K + , Zn 2+ Weigh an appropriate amount and prepare 10 mL of a standard solution with a concentration of 0.1 mol / L with ultrapure water. (1) Add concentration of 1.0×10 to a 10.0 mL volumetric flask -4 mol / L Probe S aqueous solution 1.0 mL, 1.0×10 -3 mol / L carbendazim standard solution 0.1 mL, add 0.1mol / L HCl aqueous solution to adjust the pH=2.0, distilled water to the mark, shake well, and let it stand at room temperature for 10 minutes, at this time, probe S, carbendazim mole The concentration ratio is 1:1, and the molar concentration is 1.0×10 -5 mol / L; (2) Add concentration of 1.0×10 to a 10.0 mL volumetric flask -4 mol / L Probe S aqueous solut...

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Abstract

The invention relates to a method for measuring carbendazim in water by using a supramolecular complex fluorescence probe, belonging to the technical field of analytical chemistry. According to the principle that fluorescence quenching of a dye acridine orange can be caused according to a Cucurbit(8) uril supramolecular complex generated by Cucurbit(8) uril and acridine orange and the fluorescence intensity of the Cucurbit(8) uril supramolecular complex can be enhanced by virtue of carbendazim, the Cucurbit(8) uril supramolecular complex formed by the Cucurbit(8) uril and acridine orange according to the molar concentration ratio of 1:1 serves as a fluorescence probe, the fluorescence intensity at the fluorescence emission wavelength of 529nm is obviously enhanced after the carbendazim is added into the fluorescence probe solution, change of the fluorescence intensity of the probe and the concentration of the carbendazim have a good linear relation in a certain range, and the residual carbendazim in the water can be detected. According to the method, the carbendazim in the water can be simply, rapidly and sensitively detected, and a novel method is provided for detecting pesticide residues.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and specifically relates to a method for measuring carbendazim in water by using a supramolecular complex of an eight-membered melon ring and acridine orange as a fluorescent probe. Background technique [0002] Fluorescent molecular probes are favored due to their high sensitivity, low cost of testing, simple sample processing and operation, fast determination methods, no need for expensive instruments and equipment, and easy visualization and real-time detection. Carbendazim is a kind of fungicide widely used in agricultural production. It degrades slowly in the natural environment, has certain toxic and side effects on humans and animals, and has adverse effects on human health. It is a weak fluorescent substance in chemical analysis. The currently reported carbendazim residue analysis methods mainly use high performance liquid chromatography or liquid chromatography-mass spectrom...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 黄英刘青唐青陶朱
Owner 中知在线股份有限公司
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