Fluorescent staining solution for gynecology department

Abstract

The invention discloses a fluorescent staining solution for gynecology department, which is characterized in that a fluorescent staining solution A liquid reagent component is prepared from the following components: 4 ', 6-diamidin-2-phenylindole, proclin300 and purified water; a fluorescent staining solution B liquid reagent component is prepared from the following components: acridine orange, sodium dihydrogen phosphate, disodium hydrogen phosphate, purified water and proclin300. According to the fluorescent staining solution for gynecology department, the storage condition of a reagent is reduced, and the technological process of the reagent is optimized; the validity period of the reagent is prolonged, the reagent cost is reduced, the accuracy of gynecological leucorrhea detection is improved, and the missed diagnosis rate and the misdiagnosis rate of gynecological leucorrhea specimens are reduced.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a gynecological fluorescent staining solution. Background technique [0002] Vaginitis is a general term for a variety of vaginal mucosal inflammatory diseases caused by different etiologies. In normal physiological state, the tissue anatomy and biochemical characteristics of the vagina are sufficient to defend against the invasion of external microorganisms. If it is destroyed, pathogenic bacteria can take advantage of the opportunity to enter and cause vaginal inflammation through various factors. [0003] Vaginitis is the most common disease in obstetrics and gynecology patients. It can be divided into specific and non-specific vaginitis. Most of them are bacterial vaginitis, fungal vaginitis, and trichomonas vaginitis caused by Gardnerella, fungi, and trichomonas infection. . Bacterial vaginosis is one of the most common types of vaginitis in women of childbearing ...

AI Technical Summary

Problems solved by technology

[0010] (1) Traditional wet film method: smear observation after dilution with normal saline, the wet film method is convenient and economical, and can be completed within a few minutes, but the observation of clue cells requires experience, the background is complex, it is easy to miss, and the overall positive detection rate is low and no way to check for dead trichomonas

[0011] (2) Gram staining: cells and microorganisms can be colored, and the shape after staining is clearer than that of wet slides, but trichomonas will be broken, and the background is relatively complex, so it is easy to miss or misdetect

[0012] (3) Enzyme chemistry method: It can be equipped with fully automated equipment and has a high degree of automation, but there are many interference factors of enzymes, no international authoritative report support, low clinical compliance rate, high probability of false positive and false negative, and complicated operation, which takes 45 minutes. Incubate at 37°C, cold chain transport

[0014] (5) PCR method: It has the advantages of specificity, sensitivity, high yield, quickness, simplicity, good repeatability, and easy automation, etc., but the operation requirements are high, the steps are complicated, and special PCR laboratories and professional laboratory physicians are required, and the detection cost is low. high

[0015] The key to the treatment of vaginitis is to accurately determine the pathogen of vaginitis, and microscopic examination is the gold standard for the diagnosis of vaginitis, but there are relatively many limitations in the detection of wet slides or ordinary staining. However, the general fluorescent staining solution has problems such as poor dyeing effect, fast quenching of fluorescence effect after staining, and unstable storage of the staining solution for a long time, resulting in low fluorescence intensity after staining.

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