82results about How to "Reduce storage conditions" patented technology

The invention discloses a blood collecting container, and particularly relates to a container for collecting nucleic acid preserved at normal temperature. The container comprises a collecting pipe and a rubber plug, wherein the rubber plug is sealed on a pipe orifice of the collecting pipe; a protective cap is arranged outside the rubber plug; an additive is arranged inside the collecting pipe; and the additive is prepared from a blood anticoagulant and a buffer solution thereof, an antiseptic fixative, a nuclease inhibitor, a metabolic inhibitor and a cell membrane stabilizer in a mixing manner. By adopting the container, the collected sample can be kept in an initial state for 7-14 days or even longer time at normal temperature, and does not decay, so that the detection result is not affected. The collecting container can be applied to collection, storage and transportation of a blood sample in a disaster, a war or a hard area, can be applied to collection, storage and transportation of other tissues, cells and the like in a scientific experiment, and also can be applied to other clinical inspection items. By adopting the container disclosed by the invention, the storage condition after the sample is collected is reduced. Thus, the labor of a worker is reduced, and the work efficiency is improved.

A natural anti-staling agent and filming agent for crystal pork is proportionally prepared from nisin, thia-phenol, lysozyme and filming agent chosen from glacic acetic acid- chitosan mixture and callegen or pollutant polyose.

The invention discloses a blood sample collecting device. The collecting device comprises a collecting tube, wherein an orifice of the collecting tube is provided with a sealing rubber plug, the outside of the rubber plug is provided with a protective cap, the collecting tube is filled with additives, the additives are composed of a blood anticoagulation, a hemocyte nucleic acid stabilizing agent, a blood plasma nucleic acid stabilizing agent, and a pH buffer solution; the blood anticoagulation is one or more of oxalate, heparinate and citrate, the pH buffer solution is one of glycine-sodium hydroxide-hydrochloric acid buffer solution; the hemocyte nucleic acid stabilizing agent is one or more of allantoin, 5,5-dimethyl hydantoin and oxazolidine; the blood plasma nucleic acid stabilizing agent is N-acetic acid azomethine, N-(3-acetic acid-amylamine)azomethine. The blood sample collecting device simultaneously contains the hemocyte nucleic acid stabilizing agent and the blood plasma nucleic acid stabilizing agent, and does not contain free aldehyde material, so that the blood sample can be stored in long term at normal temperature, and the free nucleic acid level of the blood sample can be stabilized, and the completeness of the free nucleic acid can be maintained.

The invention belongs to preservation agents of blood samples and in particular relates to a blood preservation agent for protecting free DNA. Every 100ml of the preservation agent comprises the following components: 6.0-8.8g of an anticoagulant, 0.1-2.0g of a natural preservative, 4.8-11.7g of a DNA enzyme inhibitor, 5.0-10.0g of glucose, 5.0-8.0g of an antihemolytic agent, 0.1-0.5g of purine and the balance of buffer solution, totaling 100ml. The preservation agent disclosed by the invention can achieve the effects of effectively preventing blood cells from rupturing to release genome DNA so as to cause pollution, preventing degradation of free DNA in the plasma and providing a stable environment for the free DNA in the plasma. Moreover, the preservation agent is safe and non-toxic to the human body and environment and is favorable for accurate detection and analysis of the free DNA.

A stable liquid formulation includes a fusion protein having an Fc domain of a human immunoglobulin G (IgG), in particular, a protein in which an Fc domain of a human immunoglobulin G (IgG) and a soluble extracellular domain of a vascular endothelial growth factor (VEGF) receptor are fused (e.g., aflibercept)). A composition for stabilizing a protein and a method for stabilizing a protein in which an Fc domain of an IgG and a soluble extracellular domain of a VEGF receptor are fused are disclosed. The present invention improves therapeutic effects on various ophthalmic diseases (e.g., retinal vein occlusion, diabetic macular edema, choroidal neovascularization and wet age-related macular degeneration, etc.) caused by abnormal angiogenesis, while pursuing stabilization of bioactivity through a stable liquid formulation suitable for intravitreal injection of an anti-VEGF-Fc fusion protein including aflibercept.

The invention provides a gold deposition detection method for a gene chip. The method is characterized by comprising the steps of carrying out sample application on an existing DNA sequence to a micro array to prepare the gene chip, previously decorating a biological macromolecule at the 5' end of nucleic acid to be detected, marking the other biological macromolecule matched with a decorative molecule on the nucleic acid to be detected on nanogold to establish a nanogold composite probe, then mixing the decorated nucleic acid to be detected, the marked nanogold composite probe and the gene chip, incubating, washing the unreacted nanogold composite proble and adding hydrogen peroxide gold to enhance reaction liquid observation. The gold deposition detection method for the gene chip has the advantages of being high in flexible, convenient to detect, simple to operate, short in consumed time, low in cost, visual in detection and low in relying degree on detecting and signal reading equipment.

The invention discloses a porcine Delta coronavirus detection kit based on a constant temperature isolation type fluorescent polymerase chain reaction (PCR) platform, and application of the porcine Delta coronavirus detection kit. The porcine Delta coronavirus detection kit consists of reaction buffer, a fluorescent quantitative PCR reaction liquid freeze-drying tube, a positive reference substance freeze-drying tube and a negative reference substance freeze-drying tube, wherein the fluorescent quantitative PCR reaction liquid freeze-drying tube is formed by mixing and freeze-drying Taq enzyme, reverse transcriptase, a detection primer, a probe and deoxynucleoside triphosphate (dNTP). The kit provided by the invention is short in detection time (the reaction time is only 42min), high in specificity and sensitivity and convenient to store (at 4 DEG C); when being matched with a constant temperature isolation type PCR instrument for use, the kit is very suitable for the rapid field detection of the porcine Delta coronavirus and can be widely popularized at the grassroots level.

The invention belongs to the technical field of quantum imaging, and discloses a steady quantum sparse imaging system and a method. The system comprises a laser, a half-wave plate, BBO (Barium Boron Oxide) crystal, a first focusing lens, a beam splitter, a second focusing lens, a spatial light modulator, a first single photon detector, a second single photon detector, a time correlation single photon counting card and a computer. Under the situation of interference and system errors, imaging robustness can be guaranteed.

The invention discloses a yak rotavirus detection kit based on a constant-temperature isolation type fluorescence PCR (Polymerase Chain Reaction) platform and application. The yak rotavirus detection kit is composed of a reaction buffering solution, a fluorescence quantitative PCR reaction solution freeze-drying pipe, a positive reference substance freeze-drying pipe and a negative reference substance freeze-drying pipe. The fluorescence quantitative PCR reaction solution freeze-drying pipe is formed through mixing and freeze-drying a Taq enzyme, reverse transcriptase, a primer for detecting, a probe and dNTPs (deoxyribonucleoside triphosphate). The kit has the advantages of short detection time (reaction time is only 42min), high specificity, high sensitivity and convenience for storage (preservation at 4 DEG C); the kit is matched with a constant-temperature isolation type PCR instrument, is very suitable for on-situ rapid detection of yak rotaviruses and can be widely popularized in grassroots.

The invention discloses a composition for resisting swine foot-and-mouth disease, freeze-dried powder, and a preparation method and use of the freeze-dried powder, and belongs to the technical field of veterinary feed additives. The composition comprises, by weight, 95-100 parts of a swine foot-and-mouth disease-resistant yolk antibody and 1-5 parts of a swine foot-and-mouth disease-resistant transfer factor. The freeze-dried powder comprises, by weight, 95-97 parts of the swine foot-and-mouth disease-resistant yolk antibody, 1-2 parts of the swine foot-and-mouth disease-resistant transfer factor, 0.2-0.3 parts of an inactivator, 0.01-0.02 parts of an antiseptic and 2.05-3.6 parts of a heat-resistant freeze-dried protective agent, and has the advantages of low preservation conditions, long preservation time, high antibody titer and good biological activity. Through combined use of the swine foot-and-mouth disease-resistant yolk antibody and the swine foot-and-mouth disease-resistant transfer factor, the freeze-dried powder has effects of preventing and treating swine foot-and-mouth disease, improves swine immunity and survival rate, is convenient for use and has a low cost.

The invention discloses a heat-resisting cryoprotectant of a mink canine distemper live vaccine. A solution is prepared by using water for injection as a solvent. Components of the heat-resisting cryoprotectant comprise, by mass, 3% of gelatin, 5% of polyvinylpyrrolidone, 1% of dehydrated fructose, 3% of trehalose, 3% of glycine, 3% of sorbitol, and 2% of sodium glutamate. The components are all dry matters. The preparation method comprises: (1) dissolving the gelatin and the polyvinylpyrrolidone (K90) which can be autoclaved into the water for injection according to their respective percentage, and sterilizing the obtained solution for 15 minutes at a temperature of 121 DEG C; (2) dissolving the components which can not withstand high temperature, such as the sorbitol, the sodium glutamate, the dehydrated fructose, the glycine, the trehalose, and the saccharose, into the water for injection according to their respective percentage, and removing bacteria by filtering through a filter membrane with a bore diameter of 0.22 mum; and (3) mixing the solutions obtained in the step (1) and the step (2) to obtain the heat-resisting cryoprotectant. The invention provides a heat-resisting cryoprotectant of mink canine distemper live vaccine which is a low cost and long-acting product, and the preparation method thereof.

An adiponectin detection kit comprises a chromogenic reagent R1 and a chromogenic reagent R2. The chromogenic reagent R1 comprises sodium acetate, citric acid and 30% hydrogen peroxide. The chromogenic reagent R2 comprises ethylenediamine tetraacetic acid disodium, citric acid, glycerol, TMB (tetramethyl benzidine) and ethanol. As the combination of sodium acetate and citric acid in the chromogenic reagent R1 forms an acid buffer solution, and hydrogen peroxide has a relatively high oxidation effect in an acid environment, the chromogenic reagent R1 can be stored for a long time without growth of microorganisms such as bacteria, fungi and the like and can still guarantee the normal effect in a test process. Meanwhile, ethylenediamine tetraacetic acid disodium and citric acid in the chromogenic reagent R2 are matched to form the buffer solution, therefore, the pH value of the chromogenic reagent R2 can be relatively stable; besides, ethylenediamine tetraacetic acid disodium can be complexed with heavy metal, so that preservation time of the chromogenic reagent R2 is prolonged, interference in adiponectin detection results from the heavy metal is also reduced, and sensitivity of the kit is improved.

The invention relates to a method and device for preserving tissues, cells, organs and body fluid of an animal body or a plant body. The method comprises the steps of putting disinfected tissues, cells and organs of the animal body or the plant body into a disinfected preservation device; then, putting the preservation device into a freezing dryer, and carrying out freeze drying on the preservation device; then, vacuumizing the preservation device; carrying out hot melt sealing on a discharge hole of the preservation device while the preservation device is maintained to be in a vacuum state, and removing a melt at the upper end of the discharge hole and a sealing plug in a wire-drawing way to enable the melt to become a sealed whole; then, storing the melt for a long time. The tissues, cells and organs of the animal body or the plant body are treated in a sealing way after being treated by freeze drying, so that the tissues, cells and organs of the animal body or the plant body are isolated from the air of the outside so as to be conveniently stored at the room temperature, the preservation conditions and preservation cost of the tissues, cells and organs of the animal body or the plant body are greatly reduced, and convenience is provided for promotion and application of the technology.

The invention relates to the technical fields of chemical engineering and pharmacy, in particular to a preparation method for ipratropium bromide. The preparation method comprises the following stepsof using N-isopropylnortropine or N-isopropyl tropine as the raw material, and respectively performing methylation reaction with iodomethane, so as to obtain an iodine-containing compound; performingdeiodination reaction on the iodine-containing compound by special metal oxide, metal salt or bromine, and performing follow-up treating, so as to obtain the ipratropium bromide. The preparation method has the advantages that the storage and use condition of the methylation reagent raw material is greatly reduced, so that the transportation and storage cost of the raw material is reduced; comparedwith the bromomethane, the iodomethane has stronger reaction activity, and the reaction conditions are milder, so that the usage amount of the methylation reagent in the methylation reaction is reduced; the safety, operability and reaction effect of the original technology are enhanced by the preparation method, and the requirement on equipment is reduced.

The invention discloses a production method for a white tea cake, and the method includes the following steps: (1) picked fresh white tea leaves are added with water until the water content is 60 to 65 percent; (2) the tea leaves are piled to be fermented, and pile temperature is controlled at 60 DEG C to 70 DEG C until the water content is not more than 20 percent; (3) after pile fermentation, the tea leaves are die-pressed into a tea cake with a predetermined shape, and after drying, the white tea cake is obtained. The method breaks through the limitation that traditional white tea can only be loose tea, the produced white tea cake is convenient to carry, and tea leaf storage conditions are decreased.

The invention relates to a preparation method of an acinetobacter baumannii infection fast detection card based on multiple epitope fusion antigens. The detection card consists of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC plate, wherein colored latex microsphere coupled anti-acinetobacter baumannii surface protein multiple epitope fusion antigen polyclonal antibodies are sprayed and coated on the combination pad; and the nitrocellulose membrane comprises detection lines coated with anti-acinetobacter baumannii surface protein multiple epitopefusion antigen polyclonal antibodies and quality control lines coated with goat anti rabbit IgG antibodies. When acinetobacter baumannii is contained in an added sample, the acinetobacter baumannii firstly forms a compound with latex-rabbit anti-acinetobacter baumannii surface protein polyclonal antibodies, and is captured when migrating to detection lines coated with the anti-acinetobacter baumannii surface protein polyclonal antibodies under the capillary action; and the detection lines show corresponding colors, so that whether the acinetobacter baumannii is contained in the sample or not can be detected. The detection card has the advantages of high speed, simplicity, high speed, high sensitivity and high specificity.

The invention relates to an oil extraction technology, in particular to a preparation method of oxidation-resistant camellia oil. According to the method, fresh camellia seeds are selected and are dried in the air to remove moisture; then, extraction and filtering are sequentially performed; apple polyphenol and vitamin E are added; through ultrasonic homogenization, finished product oil is obtained. The flavor ingredients in the camellia are effectively stored; the oxidation resistance of the camellia oil is greatly improved; the nutrition ingredients are enriched; the uniformity of the camellia oil is improved; the storage period of the camellia oil is prolonged.

The invention discloses pueraria lobata feed as well as a preparation method and an application thereof. The pueraria lobata feed is prepared from 55%-70% by weight of pueraria lobata, 20%-30% by weight of fermentation additives and 10%-15% by weight of mixed microorganisms through anaerobic fermentation, wherein the fermentation additives comprise spirulina extract, ginkgo biloba extract, fructuslycii extract, bran, 5%-10% calcium chloride, manganese sulfate and ferrous chloride; the mixed microorganisms comprise cellulose decomposition bacteria, flavobacterium, bacillus subtilis and lactobacillus plantarum. The pueraria lobata feed has the advantages as follows: the obtained pueraria lobata feed not only has acid fragrance, but also has low requirement for storage, feed palatability isimproved, appetite of animals is stimulated, and feed intake is increased.

The invention discloses an aerosol automatic fire extinguishing system, comprising a computer, a core switch, a fire detector, an audible and visual alarm, a spraying indicator lamp, an emergency start-stop button, a manual start button, system wiring, a fire alarm controller, and an aerosol fire extinguishing device. Under the application background that a hot aerosol fire extinguishing agent has the advantages of no toxicity, no pollution, no corrosion, no pollution, high fire extinguishing efficiency and the like, a hot aerosol spraying device and an intelligent control system are matched, and a system capable of automatically monitoring the fire behavior and controlling the aerosol fire extinguishing device in the corresponding area to spray aerosol to extinguish fire is designed. The fire extinguishing efficiency is high; the flexibility is high; the precision is high; and compared with a traditional fire extinguishing system, a large number of pipe networks do not need to be laid, and therefore the construction labor cost and the material cost are saved.

The invention relates to the technical field of fresh-keeping of vegetables, and concretely relates to a fresh-keeping method for Chrysanthemum coronarium. The method comprises the following steps: (1) sterilization; (2) coating of an orange essential oil/capsicum essential oil/tulip essential oil complex agent; (3) high static pressure treatment; (4) coating of a porous membrane; (5) coating of salicylates; (6) preservation at a normal temperature. After treatment, respiration intensity of Chrysanthemum coronarium is obviously inhibited during storage period, decrease of mineral matters, VC,chlorophyll and other nutrients in the later period of storage are substantially delayed, and accumulation of ethene content during the storage period is substantially inhibited; weight loss due to internal respiration and surface transpiration in the storage period is reduced, peroxidase and other activities are reduced at the same time, aging process of Chrysanthemum coronarium is effectively reduced, original freshness and color of leaf vegetables are better kept, and eating flavor is better.

The invention relates to a preparation method of latex immunochromatography test paper for rapidly detecting moraxella catarrhalis. The test paper is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC plate, the combination pad is coated with an anti-moraxella catarrhalis surface protein polyclonal antibody coupled by colored latex microspheres, and the nitrocellulose membrane is coated with a detection line of an anti-moraxella catarrhalis surface protein polyclonal antibody and a quality control line of a goat anti-rabbit IgG antibody. When the added sample contains the moraxella catarrhalis, the moraxella catarrhalis and the latex-rabbit anti-moraxella catarrhalis surface protein polyclonal antibody firstly form a compound, the compound is captured when being migrated to a detection line coated with the moraxella catarrhalis surface protein polyclonal antibody under the capillary action, and the detection line shows a corresponding color, so that whether a sample contains the moraxella catarrhalis or not can be detected. The test paper has the advantages of rapidness, simplicity, high sensitivity and good specificity.