Gold deposition detection method for gene chip
A detection method and gene chip technology, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of limited kit development, high toxicity of silver staining reagents, strong light sensitivity, etc., and improve detection sensitivity. , Simple operation, high detection sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0035] Example 1: Comparison of results of detection of Mycobacterium tuberculosis by gold deposition detection method based on hydrogen peroxide reduction, hydroxylamine reduction and silver staining gene chip
[0036] 1. Chip preparation
[0037] Use the chip spotting instrument to dot the amino modified probe (see Table 1 for probe sequence) on the surface of the aldehyde modified chip, and place the dot matrixed chip in a confined space with a relative humidity of 75% and a temperature of 30℃ for fixation 24 ~48 hours.
[0038] Table 1 Sequence list of nucleic acid probes
[0039]
[0040] Note: Biotin: biotin; NH2: amino, all sequences are synthesized by Takara
[0041] 2. Preparation of nano gold composite probe
[0042] Use 0.1mol / L K 2 CO 3 Adjust the pH of the nano-gold solution (20μl / ml) to pH6, and add 1 / 10 volume of streptavidin solution (60μg / ml) (concentration is determined by the nano-gold protein stability experiment). Mix at room temperature for 20min~2h, then add a ce...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap