Method for preparing fast detecting test paper of illegal cooking oil

A technology for detecting test strips and waste oil. It is used in measurement devices, instruments, scientific instruments, etc. It can solve the problems of cumbersome, requiring very professional personnel to operate, not suitable for rapid on-site screening, and prone to false positives. Simple, reduced detection cost, and high sensitivity

Inactive Publication Date: 2013-02-13
深圳市宝凯仑科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, according to the characteristic components of waste oil, the detection methods of waste oil mainly include moisture content determination, acid value determination, cholesterol content determination, near infrared spectroscopy , conductivity and polar substance determination method, and heavy metal content determination method; the above methods have their own advantages in application, and can perform qualitative and quantitative detection of waste oil to a certain extent, but there are also defects: such as cooking oil mixed with waste oil The proportion generally needs to reach a relatively high level to be detected; the detection limit of the conductivity method is that the amount of waste oil added is more than 20%, and the defect of the cholesterol method is that the detection limit of the method is that the amount of waste oil added is 10% The above, and the possibility of false positives in the detection is relatively large; thin layer chromatography is prone to false positives for some unpurified oils and oils with other ingredients; for some detection accuracy is relatively high compared to the above methods, Such as: Shenzhen Institute of Drug Control, HPLC detection of cholesterol content, the minimum detection limit is 20 μg / ml; Shenzhen Institute of Drug Control, LC-MS method for detection of DBS (sodium dodecylbenzene sulfonate), the detection limit is 20 μg / ml; Tsinghua University, Paramagnetic resonance spectroscopy, detection of free radicals, etc., requires large and expensive detection instruments, and takes a long time. It is more suitable for laboratory confirmation and is not suitable for on-site rapid screening; Shanghai Zhijiang Biomedical Technology Co., Ltd. uses Real-time fluorescence quantitative PCR amplifies animal-specific gene fragments to qualitatively detect gutter oil, which has high sensitivity (can detect the lowest content of 1‰), but the whole operation process is cumbersome and requires very professional personnel to operate; Guangzhou Center for Disease Control and Prevention uses immunoassay Fluorescence detection method detects bacterial fat-soluble protein and lipopolysaccharide, but this method can only judge waste oil from the aspect of a large number of bacteria in swill; therefore, a new rapid detection technology should be developed for the qualitative and Quantitative detection is imminent

Method used

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  • Method for preparing fast detecting test paper of illegal cooking oil
  • Method for preparing fast detecting test paper of illegal cooking oil
  • Method for preparing fast detecting test paper of illegal cooking oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of immunochromatic latex microspheres.

[0017] 1. Pretreatment of colored latex microspheres.

[0018] Take red, yellow, blue, and green four-color latex microsphere solutions with a diameter of about 100nm, uniform particle size, and a coefficient of variation <10%. After 5 minutes of ultrasonic treatment at 80Hz, adjust the concentration to 1% with ultrapure water, 10000g ×10min centrifugation, the precipitate was washed twice with 25mM, pH6.0 MES buffer for later use. Use pre-cooled 25mM, pH6.0 MES buffer solution to prepare EDC and NHS into 50mg / ml solution for later use, add 165μl DEC solution and 165μl NHS solution per ml latex, add EDC and NHS to the washed latex . Mix slowly at room temperature for 30 min; centrifuge at 10,000 g × 10 min after incubation, and wash the precipitate twice with an equal volume of 25 mM, pH 6.0 MES buffer.

[0019] 2. Antibody labeling.

[0020] Dilute antibody A (mouse anti-aflatoxin monoclonal ant...

Embodiment 2

[0021] Example 2 Performance evaluation of color latex microsphere immunochromatographic detection test strip.

[0022] 1. Determination of the minimum detection limit of the test strip.

[0023] At room temperature, prepare aflatoxin B1 standard, benzopyrene standard, anisidine standard and DBS standard into solutions with a series of concentrations (1-243ng / ml), and add 100 μl to the microwells of the microtiter plate , use waste oil color latex microsphere immunochromatography test strips to analyze in turn, repeat 3 times; through visual observation, the minimum standard concentration when the color of the test line of the test strip is obviously lighter than that of the negative control strip, is The detection limit of the test strip is determined by naked eyes; the detection limit of aflatoxin is 7ppb, the detection limit of benzopyrene is 9ppb, the detection limit of anisidine is 9ppb, and the detection limit of DBS is 5ppb.

[0024] The results are shown in Table 1:...

Embodiment 3

[0030] Example 3 Detection of samples and judgment of results.

[0031] 1. Processing of samples to be tested.

[0032] use figure 2 The shown device 1 draws the edible oil sample, squeezes it gently, squeezes out 2 drops of the oil sample into the No. 1 tube of the device 3, and uses the device 2a to absorb the redistilled petroleum ether in the No. 2 tube of the device 3 and squeezes out 4 drops Put it into tube No. 1, use device 1 to blow and mix the liquid in tube 1, then use device 2a to suck up the 80% methanol water (v / v) solution of tube 3 in device 3 and squeeze out 4 drops to tube 1 In the same way as above, mix evenly, let it stand until the liquid is clearly separated, use the device 2a to carefully absorb the upper liquid and discard it, use the device 2b to absorb the pure water from the No. 4 tube of the device 3, and squeeze out 16 drops to the No. 1 tube. Mixed to be tested.

[0033] 2. Sample testing.

[0034] Insert the end of a test strip with the ma...

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Abstract

The invention provides a method for preparing fast detecting test paper of illegal cooling oil. The method includes the following steps of sequentially preparing immune colored latex microspheres, an immune colored latex reaction pad and a nitrocellulose (NC) membrane and assembling a colored latex test strip. By means of the detecting test paper of the illegal cooking oil, detection sensitivity is high, a plurality of indexes can be simultaneously detected, a comprehensive and systematic detection method is provided for detecting the illegal cooking oil, and detection costs are relatively reduced; the quality of the detecting test paper can be guaranteed for two years at normal temperatures, the quality guarantee period is effectively prolonged, and preservation conditions are reduced; non-professional persons can use the detecting test paper to accomplish whole detection, so that the operation is simple, and popularization of the method is facilitated; and the whole detection process can be accomplished in 10 minutes at the soonest, and the detecting test paper is suitable for fast field screening.

Description

technical field [0001] The invention relates to the technical field of waste oil detection, in particular to a preparation method of waste oil rapid detection test paper. Background technique [0002] Waste oil is also known as swill oil, swill oil or table recycling grease, which is a general term for various low-quality oils; waste oil has experienced repeated high-temperature frying and sewer oxidation and corruption, and its quality and hygiene are extremely poor. Changes occur, resulting in various toxic substances such as aflatoxin and benzopyrene, and contain late-stage pollutants such as detergent components, such as long-term consumption, which will inevitably cause physical diseases. In mild cases, it can cause indigestion and diarrhea, and in severe cases, it can cause symptoms such as anemia and toxic liver disease. Long-term consumption can cause cancer, which is extremely harmful. In recent years, waste oil incidents are common. According to statistics, there a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 李攀谈宇清
Owner 深圳市宝凯仑科技有限公司
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