The invention relates to a detection method of a pharmaceutical preparation for curing
stomach pain caused by qi stagnation. The detection method comprises the following cinene content measuring steps: (1) taking 2.0-3.0g of pharmaceutical preparation to be detected, adding 10-100mL of 30%-100%
methanol, weighing, carrying out ultrasonic or heating
reflux for 10-60min, cooling, weighing again, supplementing the
lost weight by using 30%-100%
methanol, shaking uniformly, and filtering, thereby obtaining filtrate which is taken as a sample solution; (2) taking a cinene reference substance, adding
methanol to prepare a solution in which every 1mL of methanol contains 0.05-0.15 micro-
liter of cinene, wherein the solution is taken as a reference substance solution; (3) implementing chromatographic conditions, wherein C18 is taken as a
chromatographic column, a chemically bonded phase is taken as a fixed phase, a
mixed solution with the volume ratio of
acetonitrile to water being (60-80) to (40-20) is taken as a mobile phase, the time is 20-40min, the
column temperature is 10-30 DEG C, the detection
wavelength is 210nm, and the flow rate is 0.5-1.5mL / min; and (4) respectively precisely sucking 6-10 micro-
liter of sample solution and 6-10 micro-
liter of reference substance solution, and measuring by injecting the solutions into high performance liquid chromatograph. The detection method is rapid and comprehensive, is strong in pertinence, is beneficial to effective control on quality of the pharmaceutical preparation and also is beneficial to improving the use safety and stability.