Analysis device for integrated nucleic acid extraction and amplification detection based on smart phone and method thereof

A smart phone and integrated nucleic acid technology, which is applied in the field of amplification, integrated nucleic acid extraction, and detection analysis devices, to achieve the effects of strong versatility, high temperature control accuracy, and fast heating and cooling speed

Active Publication Date: 2020-05-26
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the defects of traditional portable nucleic acid detection equipment, the present invention provides an analysis device for integrated nucleic acid extraction, amplification and detection based on smart phones

Method used

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  • Analysis device for integrated nucleic acid extraction and amplification detection based on smart phone and method thereof
  • Analysis device for integrated nucleic acid extraction and amplification detection based on smart phone and method thereof
  • Analysis device for integrated nucleic acid extraction and amplification detection based on smart phone and method thereof

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Embodiment Construction

[0049] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes and modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0050] Such as figure 1 As shown, an analysis device based on a smartphone that integrates nucleic acid extraction, amplification, and detection consists of a smartphone 1, a micro-control unit 2, a microfluidic drive unit 3, a temperature control unit 5, a fluorescence detection unit 6, and a microfluidic control unit. Chip 4 composition.

[0051] The smart phone 1 sends control commands to the micro-control unit 2 t...

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Abstract

The invention discloses an analysis device for integrated nucleic acid extraction and amplification detection based on a smart phone and a method thereof, which relates to the key technical field of intelligent medical instruments. According to the analysis device, the smart phone is used as a main component, the smart phone is integrated with microfluid driving, temperature control and multicolorfluorescence detection, a portable multifunctional universalization device integrating nucleic acid extraction, amplification and detection is formed, the device is combined with a micro-fluidic chip; on-chip nucleic acid extraction, isothermal amplification qualitative detection and detection of real-time fluorescent quantitative PCR (Quantitative Real-time PCR,qPCR) and multiple fluorescent PCR(Multiplex PCR) are realized, and analysis and detection of pathogen nucleic acid are realized. The analysis method comprises the steps of nucleic acid extraction, system configuration, system loading, temperature setting, image acquisition, image processing and result storage and sharing. The smart phone is used as a main component, the size is small, the cost is low, the functions are complete,the applicability is high, and the detection method is diversified.

Description

technical field [0001] The invention relates to the key technical field of intelligent medical instruments, in particular to an analysis device based on a smart phone that integrates nucleic acid extraction, amplification and detection. Background technique [0002] In recent years, various infectious diseases have continued to break out worldwide, such as bird flu in 2013, Zika in 2016, African swine fever in 2018, etc. These infectious diseases have seriously threatened human life and health and caused huge harm to the people. property loss, and even threaten national security, so it is of great significance to carry out instant detection of infectious diseases. [0003] The spread of infectious diseases is characterized by infectivity, prevalence, regionality, and suddenness. Therefore, detection methods for infectious diseases need to meet the requirements of on-site, instant, and accurate. At present, the detection of infectious diseases is mainly to detect the nucleic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/02C12M1/00C12Q1/6844C12Q1/6851C12Q1/686
CPCB01L3/502707C12Q1/6844C12Q1/6851C12Q1/686B01L2200/12B01L2400/0475C12Q2565/629C12Q2531/113C12Q2563/107C12Q2537/143Y02A50/30
Inventor 杨柯朱灵刘勇邓国庆朱灿灿潘井宇洪承刚胡安中
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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