The invention discloses a double fluorescent lyophilized microchip, a kit and a method for the novel coronavirus (2019-nCoV). The kit comprises the lyophilized microchip, a tube of mineral oil, a tubeof positive control, a tube of negative control, a tube of diluent and a tube of nuclease-free water, wherein the lyophilized microchip is coated with a primer, a probe, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg<2+>. According to the lyophilizing condition, the temperature of an insulator is reduced to negative 55 DEG C at a pre-freezing stage; pre-freezing is kept for 1 hour, and equipment is used for vacuumizing, and freeze-drying is kept for 1 hour; in an analytic drying stage, the temperature of the insulator is increased to negative 25 DEG C and kept for 1 hour, isincreased to 37 DEG C and kept for 2 hours and is reduced to 25 DEG C and kept for 1 hour to obtain the lyophilized microchip, and the diluent and sample nucleic acid are added during use. The doublefluorescent lyophilized microchip can be used for simultaneously detecting an ORF 1a/b gene and an N gene. The detection kit and detection method have high accuracy, high specificity, high sensitivityand short detection time.