50 results about "Blood preservation" patented technology

The invention belongs to preservation agents of blood samples and in particular relates to a blood preservation agent for protecting free DNA. Every 100ml of the preservation agent comprises the following components: 6.0-8.8g of an anticoagulant, 0.1-2.0g of a natural preservative, 4.8-11.7g of a DNA enzyme inhibitor, 5.0-10.0g of glucose, 5.0-8.0g of an antihemolytic agent, 0.1-0.5g of purine and the balance of buffer solution, totaling 100ml. The preservation agent disclosed by the invention can achieve the effects of effectively preventing blood cells from rupturing to release genome DNA so as to cause pollution, preventing degradation of free DNA in the plasma and providing a stable environment for the free DNA in the plasma. Moreover, the preservation agent is safe and non-toxic to the human body and environment and is favorable for accurate detection and analysis of the free DNA.

An apparatus and method may provide effective preservation and usage of preserved blood or blood components. A blood preservation apparatus may include a plurality of collection bags that may be separated by separator mechanisms to enable rapid separation of the collection bags from the blood preservation apparatus when the apparatus is frozen.

The invention relates to an efficient CIK cell preparation and detection method and aims at solving the problems of few proliferation times, unstable preparation process and the like of the prior art. The efficient CIK cell preparation and detection method comprises the following steps that sterile acquisition is conducted peripheral blood of a healthy person or a patient according to a lymphocyte absolute value; whole blood testing and blood sample preservation are performed; separation and inactivation of blood plasma are performed; a density gradient centrifugation method is adopted, namely a single mononuclear cell is separated out by using lymphocyte separation liquid Ficoll; in the CIK cell induction process, a factor 1 and a factor 2 are added successively, collection can be performed only through serum-free induced cell multiplication culture for about 2-3 weeks, and a CIK cell preparation is prepared. The method has the advantages of being small in blood collecting quantity, stable in preparation process, simple and convenient to operate and high in cell proliferation times and cytotoxic activity.

The invention discloses a method for extracting genome DNA of poultry, applied to large-scale collection, separation and purification of genome DNA of poultry in the actual production. Based on the condition that the poultry blood corpuscle contains a karyon, the method for extracting genome DNA of poultry in the invention proposes a principal that the DNA genome can be extracted once the poultry blood is taken out, completely saving the steps of anticoagulation and blood preservation; the method also proposes that blood is taken from the plantar vein, realizing simple and efficient blood sample collection and less stimulation to the poultry, and furthest avoiding reduction of egg and meal yield because of blood taking. The blood sample not more than 20mul in each poultry is taken on spot and then directly put into a 1.5ml centrifugal tube and mixed with the 700mul of poultry blood lysate in the centrifugal tube, put into an ice-cube box and taken back to the laboratory for DNA extraction; the whole process is secure and reliable, and biological security danger caused by massive poultry blood transport is reduced. Aiming at the problem that protein pollution easily occurs in the poultry blood extraction process, the method of the invention proposes that the DNA is extracted according to the original phenol method, but the saturated phenol is treated for two times and centrifuged at four degrees, thus the protein pollution is extremely reduced, the purification of the extracted DNA is much improved, and the subsequent molecular detection is much facilitated.

The invention discloses a hematopoietic stem cell cryopreserving method and a protective agent. The hematopoietic stem cell cryopreserving method comprises the following specific steps mixing and cryopreserving a hematopoietic stem cell suspension or a marrow fluid and a stem cell protecting agent under a sterile condition, wherein the stem cell protecting agent comprises the following components of DMSO (dimethylsulfoxide), a 1640 sterile medium and a blood preserving solution I in a volume ratio of (9-10): 5: (5-6). By the hematopoietic stem cell cryopreserving method, the cost of the hematopoietic stem cell protecting agent can be reduced; high-cost equipment such as a programmed cooling instrument, and a complicated and high-risk procedure of adding liquid nitrogen are not required; and the hematopoietic stem cell cryopreserving method is easy to operate and easy to popularize in medical institutions at all levels.

The invention provides a detection method of hormones in blood, and belongs to the field of the detection of the hormones. The detection method comprises the following steps of mixing and storing the blood and an internal standard solution including a hormone isotope internal standard on a filter paper disc, and preparing an obtained mixture into a dried blood spot. An extracting solution is obtained from the dried blood spot through extraction, and a detection solution is obtained by carrying out derivatization treatment on the extracting solution. The hormone in the detection solution is detected through liquid chromatographic-mass spectrometric detection. In the method provided by the invention, the blood is stored on the filter paper disc, and then the purpose of quickly and effectively carrying out qualitative and quantitative detection on the hormone in the blood is realized through extraction and derivatization processes and by utilizing a liquid chromatographic-mass spectrometric method. As the blood is stored on the filter paper disc and is stored in the form of a solid, a biological safety problem possibly brought about in a transportation process is avoided; moreover, the stability can be greatly improved; therefore, a detected sample is prevented from being polluted by a microorganism.

The invention discloses a blood maintenance liquid, a preparation method and application thereof. The blood maintenance liquid is an aqueous solution comprising 88.85-90.00mmol/L of sodium citrate, 15.10-16.20mmol/L of citric acid, 136.50-146.60mmol/L of waterless glucose, 14.00-14.31mmol/L of sodium dihydrogen phosphate (NaH2PO4.H2O), 5.30-5.58mmol/L of adenine and 2.20-3.00mmol/L of L-arginine, wherein the pH value of the aqueous solution is 5.5. According to the invention, a case that the blood maintenance liquid is only used for maintaining the oxygen carrying function of erythrocytes is fundamentally changed, and new functions of hemangiectasis and local blood amount increase are endowed to stored blood, so the vasodilation activity is improved, the tissue perfusion flow is increased and the tissue blood and oxygen supply is increased during blood transfusion, thereby the blood maintenance liquid of the invention, which allows the clinical blood transfusion security and validity to be improved, is a revolution of the blood preservation and the blood quality control.

The invention discloses a polyvinyl chloride material with high oxygen permeability and a preparation method thereof. The polyvinyl chloride material with high oxygen permeability comprises the following components in parts by weight: 60 parts of polyvinyl chloride resin with super-high molecular weight, 30-40 parts of polyvinyl chloride resin with middle molecular weight, 40-80 parts of plasticizer TOTM (Trioctyl Trimellitate), 5-20 parts of plasticizer DOA (Dioctyl Adipate), 0-20 parts of plasticizer DOS (Dioctyl Sebacate), 3-10 parts of secondary plasticizer and 0.5-5 parts of calcium and zinc thermal stabilizer. The basic resin used in the material disclosed by the invention is polyvinyl chloride resin which has high oil absorption and is prepared by a super-high molecular weight and middle molecular weight suspension method, the plasticizer TOTM with low mobility is selected, the cold-resistant plasticizers DOA and DOS are compounded in use, and epoxy plant oil plasticizer with the thermal stabilizing effect is used as an assistant, so that the low-temperature resistance and extraction resistance of the product are ensured, and the material has a high gas exchange rate and characteristic of high oxygen permeability and is suitable for blood storage, and particularly suitable for storage of blood platelets. The prepared PVC material with high oxygen permeability can make sure that blood platelets can be stored for 7 days at 22 DEG C, so that the material is suitable for preparation of blood platelet storage bags.

The invention relates to the technical field of blood sample preservation, and particularly discloses a free DNA blood preservative for preventing hemolysis and applications thereof. Each 100ml of theblood preservative comprises the following ingredients: 40.0-100.0g of imidazolidinyl urea, 4.0-20.0g of EDTA-3K, and 4.0-10.0g of glycine. The blood preservative of the invention can reduce the hemolysis phenomenon of blood cells, thereby reducing the release of genomic DNA which can be mixed with free DNA, protecting the short fragments of plasma free DNA from further degradation, preventing the free DNA from cross-linking with proteins, and providing stable environment for the free DNA. The blood preservative for the free DNA of the invention has a good protective effect on the plasma freeDNA, can prevent genomic DNA contamination, and provides high quality samples for downstream free DNA detection.

The invention discloses a method for monitoring blood storage period which comprises steps of: providing a temperature variation blood timer connected with blood storage device; according to the variation of holding temperature and time, after statistics match to calculate the content of ATP in red blood cell; then evaluating the rest storage time of blood and displaying the rest storage time via the temperature variation blood timer. The inventive method has the advantages of being able to keep the temperature variation blood timer with blood, of being able to collect the temperature and cumulated storage time real-time.

A method for detecting bacterial toxins of blood storage liquid in blood sampling bag includes taking Ca(OH)2 and drying it under high temperature to remove off heat source, preparing it to be saturated solution, adding blood storage liquid to dilute said saturated solution to be diluted solution of certain times and carrying out detection by utilizing king crab reagent according to gel method listed in bacterial toxins detection manner of XIE appendit on 2005 version of Chinese pharmacopeia.

The invention relates to the technical field of medical treatment, in particular to a device and a method for recovering residual blood when ECMO patients withdraw from a machine. The device comprisesan ECMO machine, VV-ECMO or VA-ECMO cannulas, an ECMO pipeline, a blood preservation liquid bag and 0.9% of physiological saline, wherein the blood preservation liquid bag can contain recovered blood, and 0.9% of the physiological saline flushes the blood in the ECMO pipeline into the blood preservation liquid bag. During withdrawal, on the basis of strict aseptic operation, the VV-ECMO/VA-ECMO cannulas are cut off and connected through aseptic connection pipes, a three-way valve is connected to two interfaces in front of a centrifugal pump, the three-way valve at the proximal end of the centrifugal pump is connected to 0.9% of the physiological saline, the three-way valve at the distal end of the centrifugal pump is connected to the blood preservation liquid bag to form a closed loop, and the ECMO machine is started to collect residual blood. According to the device, about 500-600ml of the residual blood in the ECMO pipeline can be recovered in time, and the recovered blood treated by the blood transfusion department can be returned to patients, so that the device is economical and practical; the device is safe and effective; the recovery is complete; the operation method is simple and convenient; and the waste of blood resources is avoided.

The invention discloses a container for vacuum collection of a karyotype analysis sample. The container comprises a primitive tube, a rubber plug and a protective cap, wherein the primitive tube, the rubber plug and the protective cap are assembled into the vacuated collection container. The container is characterized in that a cell medium, a blood anticoagulant, a mitogen, a serum separating medium, a mitosis repressor and a leukocyte separating medium are arranged in the primitive tube; the cell medium comprises RPMI-1640 or 199 or F10 or Eagle; the blood anticoagulant is heparinate, sodium citrate and buffer solution thereof, EDTA salt, blood preservation solution and hirudin; the mitogen is phytolectin (PHA) or concanavalin-A; and the mitosis repressor is demecolcine, colchicine and derivatives thereof. The collection container not only can be used for karyotype analysis, but also can be used for sample collection for in situ chromosomal hybridization, chromosome linkage map analysis and microseparation and cloning. The container is simple and easy to operate, and samples can be directly used for follow-up experiments after being collected.

The invention provides blood transport equipment. The equipment includes an artificial heart, an airbag wrapping the artificial heart, an air pump supplying air to the airbag, a pipe connected with the artificial heart and a blood storage bag; the airbag is controlled by a controller to intermittently supply air to the airbag, a solenoid valve is installed on the airbag, and the controller controls the solenoid valve to be intermittently opened or closed for cooperation with the airbag, so that intermittent inflation and deflation of the airbag are achieved. The artificial heart includes a high-elasticity main body and capillary channels, the capillary channels are provided with a unified inlet and a unified outlet, check valves identical in flow direction are installed on the unified inlet and the unified outlet respectively, the inlet of the capillary channels is communicated with the bottom of the blood storage bag through the pipe, and the outlet of the capillary channels is communicated with the top of the blood storage bag through the pipe. The blood transport equipment has the advantages of being scientific in design, capable of simulating human blood circulation and conducive to blood preservation.

The invention discloses a portable intelligent blood storage box which comprises a support base, a storage box body, a programmable logic controller (PLC) and a temperature sensor. Anti-skidding support feet are arranged at four corners below the support base, a charging jack is arranged on one side wall of the support base, a storage battery is arranged in the support base, and the storage box body is arranged above the support base. The portable intelligent blood storage box has the beneficial effects that due to the arrangement of the temperature sensor and a buzzing alarm, when the temperature sensor detects that the temperature inside the storage box body exceeds the temperature range of the preset temperature, the buzzing alarm is controlled by the PLC to automatically send out an alarm signal, automatic alarm management and control can be realized, and the device is practical and convenient to use. By arranging a first ultraviolet disinfection lamp and a second ultraviolet disinfection lamp, the interior of the storage box body can be subjected to ultraviolet disinfection before blood is stored, and the safety coefficient of blood storage is improved.

The invention discloses a medical test blood preservation device, which comprises a handle,a box body, a barometer, a nitrogen filling device, a stopping block, a shelf, a temperature regulator, color contrast strip, a timer and fluorescent lamps, wherein the handle is arranged on a box cover; the box cover is hinged with the box body; hooks are arranged on two sides of the upper end of the box body; the barometer is arranged on the sidewall of the box body; the nitrogen filling device and the box body communicate; the shelf is arranged in the box body; an extrusion spring is arranged on the lower surface of the shelf; the other end of the extrusion spring is connected to the bottom end of the box body; the stopping block is arranged on the sidewall of the box body; a plurality of through holes are arranged in the shelf; the temperature regulator is arranged in the box body; a thermometer is arranged on the sidewall of the box body; a transparent glass plate is arranged on the sidewall of the box body; the color contrast strip is arranged at the upper part of the transparent glass plate; and the timer is arranged on the sidewall of the box body. The blood preservation device disclosed by the invention can relieve influence of external side on internal environment of the box body so as to provide an appropriate environment for blood preservation; and the blood preservation device is capable of guaranteeing accuracy of a detection result, and the device is simple in structure, convenient to use and conducive to popularization.

The invention relates to the technical field of medical treatment and discloses a blood preservation device based on the intermittent refrigerating principle. The blood preservation device comprises apreservation box body, refrigerating layers are arranged in the preservation box body, main gears and auxiliary gears are arranged in the preservation box body, right threaded rods are movably installed at the ends, away from left threaded rods, of main threaded rods, movable bearings are movably connected to the ends, away from the main threaded rods, of the right threaded rods, squeezing rods are movably connected to the ends, away from the right threaded rods, of the movable bearings, and refrigerating boxes are movably connected to the ends, away from the movable bearings, of the squeezing rods. According to the blood preservation device based on the intermittent refrigerating principle, cold air is released through the refrigerating boxes to carry out cold preservation on blood; andmeanwhile, through reset structures in the squeezing rods, the inner refrigerating boxes can restore and close refrigerating when not stressed, intermittent refrigerating is carried out on blood storage pipes in the preservation box body, and it is avoided that the internal activity of blood is damaged as the temperature is too low.