Adiponectin detection kit

A technology for detecting kits and adiponectin, which is applied in the field of biochemistry, can solve the problems of false positive test results, cannot be stored for a long time, is not conducive to mass production and long-distance transportation of kit manufacturers, and achieves The effect of prolonging the storage time and prolonging the storage time

Inactive Publication Date: 2016-05-04
BEIJING EXPANDBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this kind of kit has relatively high sensitivity when detecting the concentration of adiponectin, the storage temperature of this kind of kit needs to be between 2-8°C, and it can only be stored for 6 months
For this reason, the storage conditions of this kind of kit are relatively strict and cannot be stored for a long time, otherwise it will cause the failure of the display reagent in the kit, which may eventually lead to false positive test results
Therefore, it is not conducive to the mass production and long-distance transportation of kit manufacturers

Method used

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  • Adiponectin detection kit

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Effect test

Embodiment 1

[0039] 1. Preparation steps of chromogenic reagent R1:

[0040] S1, take by weighing 13.9g sodium acetate and 1.6 citric acid, drop into 0.3ml concentration and be 30% hydrogen peroxide and dissolve;

[0041] S2, after the sodium acetate and citric acid are completely dissolved, transfer the solution to a 500mL volumetric flask, and distilled water to constant volume;

[0042] S3. Transfer the prepared chromogenic reagent R1 to a brown bottle and store it in the dark for 8 months at an ambient temperature of 15°C.

[0043] 2. Preparation steps of color reagent R2:

[0044] S1. Weigh 0.2 g of disodium edetate, 0.95 g of citric acid and 0.12 g of potassium sorbate, and put them into 50 mL of glycerin for dissolution to form solution A;

[0045] S2. Weigh 0.15g TMB and put it into 3mL of ethanol to dissolve to form solution B;

[0046]S3, solution A and solution B are mixed, and transferred to a 500mL volumetric flask, and distilled water is used for constant volume, and stand...

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Abstract

An adiponectin detection kit comprises a chromogenic reagent R1 and a chromogenic reagent R2. The chromogenic reagent R1 comprises sodium acetate, citric acid and 30% hydrogen peroxide. The chromogenic reagent R2 comprises ethylenediamine tetraacetic acid disodium, citric acid, glycerol, TMB (tetramethyl benzidine) and ethanol. As the combination of sodium acetate and citric acid in the chromogenic reagent R1 forms an acid buffer solution, and hydrogen peroxide has a relatively high oxidation effect in an acid environment, the chromogenic reagent R1 can be stored for a long time without growth of microorganisms such as bacteria, fungi and the like and can still guarantee the normal effect in a test process. Meanwhile, ethylenediamine tetraacetic acid disodium and citric acid in the chromogenic reagent R2 are matched to form the buffer solution, therefore, the pH value of the chromogenic reagent R2 can be relatively stable; besides, ethylenediamine tetraacetic acid disodium can be complexed with heavy metal, so that preservation time of the chromogenic reagent R2 is prolonged, interference in adiponectin detection results from the heavy metal is also reduced, and sensitivity of the kit is improved.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to an adiponectin detection kit. Background technique [0002] Adipose tissue is mainly composed of a large number of adipocytes aggregated into agglomerates, and adiponectin is an endogenous bioactive polypeptide or protein secreted by adipocytes. Adiponectin is an insulin-sensitizing hormone that can improve insulin resistance and atherosclerosis in mice; studies on humans have found that adiponectin levels can predict the development of type II diabetes and coronary heart disease, and have been shown in clinical trials Antidiabetic, antiatherogenic and inflammatory potential. [0003] At the same time, according to research findings, lower serum adiponectin levels can increase the risk of various cancers. Moreover, the concentration of circulating adiponectin is inversely correlated with the incidence of tumors. Therefore, detecting the concentration of adiponectin in human blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 田春城
Owner BEIJING EXPANDBIOTECH CO LTD
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