Blood sample collecting device

A blood sample and collection device technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of inability to stabilize the level of free nucleic acid and protect the integrity of free nucleic acid, which is conducive to promotion and popularization , save economic costs, ensure the effect of accuracy

Active Publication Date: 2015-06-03
广州维帝医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] The object of the present invention is to overcome the defect that the existing blood sample collection device cannot stabilize the level of free nucleic acid in the blood sample and protect the integrity of the free nucleic acid, and to invent a blood sample collection device, which can be stored at room temperature for a long time (7-14 days) blood samples, stabilize the level of free nucleic acids in blood samples, protect the integrity of free nucleic acids, and facilitate the accurate detection and analysis of cfDNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] like figure 1 As shown, the blood sample collection device includes a collection tube 3 and a rubber plug 2, the rubber plug 2 is sealed on the mouth of the collection tube 1, the rubber plug 2 is provided with a protective cap 3, and the collection tube 1 is provided with an additive 4 , the additive 4 is composed of a blood anticoagulant, a blood cell nucleic acid stabilizer, and a plasma nucleic acid stabilizer. In this example, the device disclosed in the Chinese invention patent "Nucleic Acid Collection Container Preserved at Normal Temperature" (application publication number CN 103789202A; application publication date 2014.05.14) was used, but the additive 4 used was different.

[0044] The collecting tube 3 is made of glass or plastic. Preferably, the glass is borosilicate glass or quartz glass; the plastic is polyethylene terephthalate (PET), polybutylene terephthalate (PBT) or polyethylene naphthalate ( PEN).

[0045] The rubber plug 2 is made of rubber. P...

Embodiment 2

[0056] like figure 1 As shown, the blood sample collection device includes a collection tube 3 and a rubber plug 2, the rubber plug 2 is sealed on the mouth of the collection tube 1, the rubber plug 2 is provided with a protective cap 1, and the collection tube 1 is provided with an additive 4 , the additive 4 is composed of a blood anticoagulant, a blood cell nucleic acid stabilizer, and a plasma nucleic acid stabilizer. In this example, the device disclosed in the Chinese invention patent "Nucleic Acid Collection Container Preserved at Normal Temperature" (application publication number CN 103789202A; application publication date 2014.05.14) was used, but the additive 4 used was different.

[0057] Said additive 4 is preferably blood anticoagulant EDTA-Na 2 .2H 2 O, blood cell nucleic acid stabilizer N-(2,5-dioxo-4-imidazolidinyl) urea, plasma nucleic acid stabilizer N-(3-acetic acid-pentylamine) formimine.

[0058] When preparing additive 4 for processing 10ml of blood s...

Embodiment 3

[0074] like figure 2 As shown in the blood sample collection device, the container includes a collection tube 3, and a sealing rubber plug 2 is detachably connected to the mouth of the tube. A membrane capsule 5 made of a microporous filter membrane that can trap cells and allow plasma to pass through. One end of the opening of the membrane capsule 5 is fixedly connected with the protective cap 1, and the position of the membrane capsule 5 is higher than the liquid level after the blood sample is collected. The membrane capsule 5 is a microporous filter membrane capable of retaining cells. The bottom of the collection tube 1 is filled with an additive 4, which is composed of a blood anticoagulant, a blood cell nucleic acid stabilizer, and a plasma nucleic acid stabilizer.

[0075] As for the size of the capsule 5, it is preferable that the capsule 5 is 2-3 cm lower than the rubber stopper, so as to ensure that the capsule 5 is not easy to be pierced by the injection needle,...

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Abstract

The invention discloses a blood sample collecting device. The collecting device comprises a collecting tube, wherein an orifice of the collecting tube is provided with a sealing rubber plug, the outside of the rubber plug is provided with a protective cap, the collecting tube is filled with additives, the additives are composed of a blood anticoagulation, a hemocyte nucleic acid stabilizing agent, a blood plasma nucleic acid stabilizing agent, and a pH buffer solution; the blood anticoagulation is one or more of oxalate, heparinate and citrate, the pH buffer solution is one of glycine-sodium hydroxide-hydrochloric acid buffer solution; the hemocyte nucleic acid stabilizing agent is one or more of allantoin, 5,5-dimethyl hydantoin and oxazolidine; the blood plasma nucleic acid stabilizing agent is N-acetic acid azomethine, N-(3-acetic acid-amylamine)azomethine. The blood sample collecting device simultaneously contains the hemocyte nucleic acid stabilizing agent and the blood plasma nucleic acid stabilizing agent, and does not contain free aldehyde material, so that the blood sample can be stored in long term at normal temperature, and the free nucleic acid level of the blood sample can be stabilized, and the completeness of the free nucleic acid can be maintained.

Description

technical field [0001] The invention relates to a blood sample collection device, in particular to a blood sample collection device for stabilizing the level of free nucleic acid in a blood sample and maintaining the integrity of the free nucleic acid. Background technique [0002] As early as 1948, free deoxyribonucleic acid (cfDNA for short) in blood was discovered, but its application in clinical medicine has not exceeded two decades. Specifically, cfDNA was shown to be present in the serum of patients with systemic lupus erythematosus. [0003] Currently, there are two hot spots in the research of cfDNA: [0004] One is to use the cfDNA significantly increased in cancer patients to diagnose cancer, and the cfDNA level is significantly increased in the serum of cancer patients. According to the survey, the concentration of cfDNA in patients with rheumatoid arthritis, colorectal cancer, breast cancer, pancreatic cancer, and head and neck cancer all increased significantl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 布丽斯曾晨光周启明杨习锋
Owner 广州维帝医疗技术有限公司
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