Heat-resisting cryoprotectant of mink canine distemper live vaccine and preparation method thereof

A heat-resistant freeze-drying and canine distemper technology, applied in antiviral agents, pharmaceutical formulations, medical preparations with inactive ingredients, etc. and ease of use, prolonging shelf life, and reducing the effect of storage conditions

Active Publication Date: 2012-08-08
青岛爱博生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In addition, the development of domestic traditional veterinary biological product protective agents i

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  • Heat-resisting cryoprotectant of mink canine distemper live vaccine and preparation method thereof
  • Heat-resisting cryoprotectant of mink canine distemper live vaccine and preparation method thereof
  • Heat-resisting cryoprotectant of mink canine distemper live vaccine and preparation method thereof

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Embodiment Construction

[0008] The present invention will be described in detail below in conjunction with specific embodiments.

[0009] A new type of heat-resistant freeze-drying protective agent for canine distemper live vaccine in mink, the proportion is: 3 g gelatin, 5 g polyvinylpyrrolidone, 1 g anhydrous fructose, 3 g trehalose, 3 g glycine, 3 g sorbitol, glutamine sodium nitrate 2 g, water for injection 80 g, can get 100 g mink canine distemper live vaccine heat-resistant lyoprotectant.

[0010] The preparation method of the heat-resistant freeze-drying protective agent of mink canine distemper live vaccine, (1) gelatin and polyvinylpyrrolidone (K90), which can be autoclaved, are dissolved in water for injection according to their respective proportions, and sterilized at 121 ° C for 15 minutes; ( 2) Sorbitol, sodium glutamate, anhydrofructose, glycine, and trehalose, which are not resistant to high temperature, are all dissolved in water for injection in their respective proportions and ster...

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Abstract

The invention discloses a heat-resisting cryoprotectant of a mink canine distemper live vaccine. A solution is prepared by using water for injection as a solvent. Components of the heat-resisting cryoprotectant comprise, by mass, 3% of gelatin, 5% of polyvinylpyrrolidone, 1% of dehydrated fructose, 3% of trehalose, 3% of glycine, 3% of sorbitol, and 2% of sodium glutamate. The components are all dry matters. The preparation method comprises: (1) dissolving the gelatin and the polyvinylpyrrolidone (K90) which can be autoclaved into the water for injection according to their respective percentage, and sterilizing the obtained solution for 15 minutes at a temperature of 121 DEG C; (2) dissolving the components which can not withstand high temperature, such as the sorbitol, the sodium glutamate, the dehydrated fructose, the glycine, the trehalose, and the saccharose, into the water for injection according to their respective percentage, and removing bacteria by filtering through a filter membrane with a bore diameter of 0.22 mum; and (3) mixing the solutions obtained in the step (1) and the step (2) to obtain the heat-resisting cryoprotectant. The invention provides a heat-resisting cryoprotectant of mink canine distemper live vaccine which is a low cost and long-acting product, and the preparation method thereof.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant for mink canine distemper live vaccine and a preparation method thereof, belonging to the field of production of virus live vaccines for animals. Background technique [0002] In the field of vaccine products, lyoprotectants and their preparation processes are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., with simple formulations and poor protective functions. If it is stored at 2°C-8°C, the storage period is only 4-6 months, and it needs to be stored below -15°C. In addition, the development of traditional veterinary biological product protective agents in China is relatively lagging behind, which limits...

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Application Information

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IPC IPC(8): A61K47/42A61K47/36A61K47/32A61K47/26A61K47/18A61K39/175A61P31/14
Inventor 黄娟单虎秦晓冰张传美杨瑞梅张洪亮
Owner 青岛爱博生物科技有限公司
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