Container for collecting nucleic acid preserved at normal temperature

Abstract

The invention discloses a blood collecting container, and particularly relates to a container for collecting nucleic acid preserved at normal temperature. The container comprises a collecting pipe and a rubber plug, wherein the rubber plug is sealed on a pipe orifice of the collecting pipe; a protective cap is arranged outside the rubber plug; an additive is arranged inside the collecting pipe; and the additive is prepared from a blood anticoagulant and a buffer solution thereof, an antiseptic fixative, a nuclease inhibitor, a metabolic inhibitor and a cell membrane stabilizer in a mixing manner. By adopting the container, the collected sample can be kept in an initial state for 7-14 days or even longer time at normal temperature, and does not decay, so that the detection result is not affected. The collecting container can be applied to collection, storage and transportation of a blood sample in a disaster, a war or a hard area, can be applied to collection, storage and transportation of other tissues, cells and the like in a scientific experiment, and also can be applied to other clinical inspection items. By adopting the container disclosed by the invention, the storage condition after the sample is collected is reduced. Thus, the labor of a worker is reduced, and the work efficiency is improved.

Description

technical field [0001] The invention is a blood collection container, especially a collection container for storing nucleic acid at room temperature. Background technique [0002] At present, the development of molecular biology is changing with each passing day. Genetic engineering is the most important discipline of molecular biology. It has been widely used in many aspects such as clinical laboratory medicine, gene targeted therapy, biopharmaceuticals, agricultural breeding and forensic identification. The basic object of genetic engineering research and application is nucleic acid, including DNA and RNA. At present, the extraction of sample nucleic acid has been developed very maturely, but the requirements for sample collection, transportation and storage conditions are relatively high, especially for RNA. Because RNA is very unstable, it can degrade rapidly and significantly. When the sample comes from clinical blood, in addition to RNA degradation, sometimes RNA is i...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect that the collection, transportation and storage of existing nucleic acids must be carried out at low temperature, and to invent a method for collecting samples at normal temperature and maintaining the initial state of the samples for a certain period of time without affecting the inspection results. A collection container for storing nucleic acids at room temperature

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