105 results about "Duck plague" patented technology

The invention relates to duck plague yolk antibody freeze-dried powder, which comprises the following components in percentage by weight: 87 to 90 percent of duck plague yolk antibody, 0.8 to 1.0 percent of octanoic acid, 0.2 to 0.3 percent of formaldehyde, 0.01 to 0.02 percent of thimerosal, 3 to 4 percent of glucose, 1 to 2 percent of sorbitol, 2 to 3 percent of glycine and 2 to 3 percent of mannitol. The freeze-dried powder has the advantages of long storage time, quick dissolution, high antibody valence and high bioactivity, and the purity of the antibody of the freeze-dried powder is higher than that of the conventional product in market. The invention also discloses a preparation method for the duck plague yolk antibody freeze-dried powder. The preparation method comprises the following steps of: separating strains to prepare vaccines, immunizing healthy ducks to obtain high-immunity eggs, performing sterilization, acidification, octanoic acid treatment, refining, extraction, purification, preparation, freeze drying and the like on the high-immunity eggs, and thus obtaining the duck plague high-immunity yolk antibody freeze-dried powder. According to the preparation method, yolk liquid is heated by using the temperature of pasteurization during acidification and water treatment, so that a sterilization effect is achieved, and the recovery rate of the antibody and the clarity of the solution during acidification can be improved at the same time; and the prepared yolk antibody has the advantages of high bioactivity, long storage time, retarded valence reducing speed and the like.

The invention discloses a preparation method of a natural high-effective biological active substance anti-duck plague virus transfer factor. The preparation method comprises the following steps of: inoculating duck plague virus into an allantoic fluid obtained from a 10-day-aged chick embryo, and preparing duck plague virus antigen; extracting the duck plague virus antigen; immunizing a pig with the extracted duck plague virus antigen; and extracting the anti-duck plague virus transfer factor from the immunized pig. The inventive anti-duck plague virus transfer factor can prevent duck plague and protect normal body cells from being infected by the duck plague virus, so as to reduce incidence rate.

The invention discloses a duck culture method, which comprises the following steps of (1) backup duck feeding: healthy ducklings are selected, then, coarse feed is used as main feed for feeding in 100th to 120th days, and fine feed is used as main feed in 140th to 160th days; (2) egg laying duck feeding: a, the dietary crude protein content is improved to 15 to 16 percent before 1 month of the egg laying, and when the daily egg laying rate reaches 30 to 40 percent, the crude protein content is improved by about 17 to 18 percent; b, the light illumination in each day is 13 to 14h, and the illumination intensity per square meter reaches 24 to 26 Lx; c, a duck plague chick embryonized vaccine is injected in each egg laying duck on 25 to 30 days before the egg laying; (3) incubation and brood time culture: after the eggs are hatched, the free food eating and water drinking is allowed in 12 to 24 hours after the ducklings go out of the shell, 6 to 8 times of little feeding and frequency feed addition are carried out every day, and the feed comprises concentrated and green feed; the in-house ventilation and dry and fresh pad materials are maintained; (4) fattening period feeding: group division is regularly carried out, sick ducklings are weeded out in time, each group includes 100 to 150 ducks, the feed is fed for 3 to 4 times every day, and sufficient moisture is supplemented.

The invention relates to a mixed yolk antibody for preventing and controlling infective oromeningitis and a preparation method thereof, and belongs to the technical field of biological agent preparation and poultry disease prevention and control. The invention takes a main epidemic serotype strain of duck plague pasteurella anatipestifer as an antigen to prepare the duck plague pasteurella anatipestifer hyperimmune yolk antibody. Infection treatment experimental results show that the prepared yolk antibody has the protection ratio for serotype I and serotype II duck plague pasteurella anatipestifer respectively reaching 80 percent and 90 percent. The product provides a new way to the prevention and control of duck infective oromeningitis and can be used for the preparation of veterinary medicines for controlling the disease or be prepared into health care products or feed additives for use. Moreover, the yolk antibody provides a material basis for improving the livestock product quality, reducing medicinal residue and promoting the human food safety.

The invention relates to the field of animal medicine, in particular to a method for detecting duck plague virus antibody. The method particularly comprises the steps of the preparation of solid phase antigen, primary antibody combination, secondary antibody combination, developing, detection, judgment and the like. The method is an indirect enzyme-linked immuno sorbent assay (ELISA) method established based on purified recombinant UL51 protein, which has good specificity, and when duck hepatitis virus (DHV), Riemerella anatipestifer (RA), duck Escherichia coli (E.coli) are detected, the detection results are all positive; intra batch or batch-to-batch repeated tests show that variation coefficients are all less than 10%, and duck plague virus (DPV) vaccine immunized duck positive sera diluted at ratio of 1:3200 can be detected.

The invention discloses a duck plague virus transfer vector pUC-deltagC-EGFP and a duck plague virus gC-deleted recombinant strain DPV-deltagC-EGFP. An escherichia coli DH5alpha containing the transfer vector pUC-deltagC-EGFP is collected on November 30th, 2011, in China Center for Type Culture Collection at Wuhan University of China, and has a collection number of CCTCC NO:M2011432. After the transfer vector is subjected to homologous recombination with the duck plague virus, and after plaque purification, the EGFP-labeled recombinant virus DPV-deltagC-EGFP is obtained. As a result of experiments, same as an attenuated vaccine, the recombinant virus DPV-deltagC-EGFP with three dosages can provide complete protection for ducklings attacked by virulent virus. Therefore, the recombinant strain has certain potential to be developed into a novel vaccine which can effectively prevent duck plague.

The invention aims at providing a duck plague virus gene deletion transfer vector and an application of the duck plague virus gene deletion transfer vector. The transfer vector is subjected to homologous recombination with duck plague virus to obtain duck plague virus gene deletion engineering strains which can be prepared into a high-safety and high-prevention-effect duck plague virus gene deletion vaccine. The duck plague virus gene deletion vaccine does not influence the virus immunogenicity while lowering down the virus pathogenicity; therefore, the prepared duck plague virus gene deletion vaccines are high in safety, able to remain the original virus immunogenicity, suitable for prevention and control for the current duck plague diseases in domestic, and beneficial for the cleaning treatment of duck plague in a duck farm; in addition, the recombined duck plague virus strains do not contain any exogenous genes; and compared with the similar duck plague virus gene vaccines reported in the prior art, the duck plague virus gene deletion vaccine has the advantage that no EGFP (Enhanced Green Fluorescent Protein), lacZ and other marker gene are contained, so that the bio-safety is improved.

The invention provides a Chinese medicinal particle for preventing and treating duck plague and a preparation method thereof. The Chinese medicine consists of the following raw materials in parts by weight: 2-4 parts of coptis root, 2-4 parts of amur corktree bark, 1-2 parts of rhubarb, 1-2 parts of dried orange peel, 1-2 parts of szechuan lovage rhizome, 1-2 parts of dangshen, 1-2 parts of isatis root, 1-2 parts of Chinese pulsatilla root and 1-2 parts of liquoric root. The Chinese medicinal particle is prepared by decocting and adding auxiliary materials. In the Chinese medicine particle for preventing and treating duck plague, the conventional Chinese medicines are combined reasonably, so that an effective treatment medicament for duck plague is provided for the duck feeding industry. The Chinese medicinal particle has the characteristics of quick response, definite curative effect and freeness from medicinal residues and toxic and side effects; and the preparation method is easy, and is suitable for the requirement of large-scale industrial production.

The present invention discloses a duck plague vaccine and a special virus strain. The active component of the duck plague vaccine is inactivated duck plague bleb virus AV1221 strain duck embryo adapted virus. The duck plague vaccine of the present invention is made from inactivated virus, which has good safety and is convenient for storing, transporting (2 DEG C to 8 DEG C) and using, and the immunity effect is stable.

The invention belongs to the field of animal medicament and biotechnology and particularly relates to a truncated duck plague virus (DPV) recombinant envelope gI protein. The DNA sequence of the truncated and expressed DPV recombinant envelope gI protein is shown as the SEQ ID No.1, and the truncated and expressed DPV recombinant envelope gI protein has a peptide sequence shown as the SEQ ID No.2. The preparation method of the truncated and expressed DPV recombinant envelope gI protein comprises the following steps: acquiring genes of the truncated and expressed DPV recombinant envelope gI protein, preparing a recombinant prokaryotic expression vector of the truncated and expressed DPV recombinant envelope gI protein, and preparing the truncated and expressed DPV recombinant envelope gI protein. The invention also relates to the application of the truncated and expressed DPV recombinant envelope gI protein to preparation of a DPV antigen or vaccine, in particular to the application of the truncated and expressed DPV recombinant envelope gI protein to the preparation of a DPV ELISA (enzyme-linked immuno sorbent assay) antibody test kit. The truncated and expressed DPV recombinant envelope gI protein has high purity, and the preparation method of the truncated and expressed DPV recombinant envelope gI protein is easy to operate, adopts the truncated and expressed DPV recombinant envelope gI protein to carry out ELISA on a DPV antibody in serum, and is simple, efficient and accurate.

The invention belongs to the technical field of veterinary medicines, and provides a medicine for treating fowl plague and duck plague. The medicine is characterized by being prepared from the following raw material medicines: 10-30 parts of antiphologistic powder, 20-40 parts of neomycin and 10-30 parts of synergist. Tests prove that the medicine can effectively treat fowl plague and duck plague, and has the advantages of being high in curative effect, time-saving and labor-saving.

The invention discloses a duck plague virus recombinant vaccine strain rDEVTK-EGFP for expressing enhanced green fluorescent protein (EGFP) genes and a constructing method and application of the duck plague virus recombinant vaccine strain rDEVTK-EGFP. The microorganism preservation number of the vaccine strain rDEVTK-EGFP is CGMCC No. 9456. According to the duck plague virus recombinant vaccine strain rDEVTK-EGFP, the constructing method and the application, a recombinant clone technology is adopted; a gene segment sCMV-EGFP containing enhanced green fluorescent proteins (EGFP) and sCMV promoter sequences is inserted into a duck plague virus TK gene region; recombinant EGFP duck plague viruses with an sCMV-EGFP expression cassette inserted into the corresponding position of a TK gene is constructed; the EGFP genes are stably expressed by the recombinant viruses. The invention further relates to a method for constructing the recombinant duck plague virus vaccine strain for stably expressing other poultry pathogeny exogenous genes and the application of the recombinant duck plague virus vaccine strain to preparation of vaccines for preventing duck plagues and other poultry infectious diseases.

The invention relates to a recombined duck enteritis virus for expressing an exogenous gene, and relates to an establishment method and application of the recombined duck enteritis virus. Particularly, a recombined duck enteritis virus carrier rDEVdeltagE-GFP which is lack of a gE gene and carries a green fluorescence marker is established, the recombined duck enteritis virus which can express the exogenous gene can be obtained through the homologous recombination method, and the foundation is laid for establishing a novel recombined vaccine resisting duck plague and other pathogeny with the duck enteritis virus as the carrier; the duck enteritis virus rDEVdeltagE-H9 strain expressing the H9 subtype avian influenza virus hemagglutinin (HA) is established. Application of the recombined duck enteritis virus (rDEVdeltagE-H9) for expressing the H9 subtype avian influenza virus hemagglutinin (HA) is used for preparing the vaccine for treating duck plague and the H9 subtype avian influenza.

The invention provides a traditional Chinese medicinal injection for treating duck plague and a preparation method thereof. The traditional Chinese medicinal injection is prepared from seven Chinese herbs, namely schisandra chinensis, calculus bovis, rhizoma polygonati, epimedium, acanthopanax, kiwi root and astragalus membranaceus, which achieve a detoxifying synergistic effect. The traditional Chinese medicinal injection has a strong antibacterial and antiviral effect, has a very therapeutic significant on the duck plague, has a curative rate up to 81%, takes effect fast, and is very suitable for control of characteristics of sudden disease occurrence, fast infection and the like of the duck plague; the duck plague is unlikely to relapse after being cured; the traditional Chinese medicinal injection is free of any toxic or side effect and safe to use.

The invention discloses a forced moulting method for improving meat breed duck production performances, and solves the problems of high death and culling rate, low fertilization rate and the like in the prior art. The method includes the steps: preparation before moulting: eliminating unqualified breed ducks, and dividing male ducks and female ducks; feed stopping: stopping feed, weighing initialbody weight eight hours after, weighing weight in seventh days and every days after the seventh days, calculating weight loss rate, regularly supplying water, and shortening illumination time; feed supplying recover: feeding ducks with different quantity of feed when the weight loss rate of the male ducks and the female ducks reaches 15% and 28%-30%, supplying water in whole day, and increasing illumination to reach 17n hours in later period; mixing groups according to ration of the male ducks to the female ducks of 1:(4.6-4.8), feeding the ducks with the feed in a time limit manner, replacingfeed by egg laying feed after ducks lay eggs, freely eating when egg laying rate is 205 or more, supplying water in whole day, and performing illumination for 17 hours; immunization: injecting vaccines such as duck plague in second weeks to sixth weeks after feed is fed. The method is mainly used for forced moulting of meat breed ducks, healthy physique of the duck groups are kept, quality of eggs in the second egg producing period is improved, and economic utilization period is prolonged.

The present invention relates to a duck plague treating traditional Chinese medicine composition and a preparation method thereof, wherein the components comprise, by weight, 1-5 parts of scutellaria baicalensis, 1-5 parts of rheum palmatum, 1-5 parts of cortex phellodendri, 1-5 parts of lysimachia fortunei maxim, 1-5 parts of rhamnus aurea heppeler, 1-10 parts of plantago depressa willd, 1-5 parts of sargentodoxa cuneata, 1-5 parts of cyperus rotundus l, 1-5 parts of radix hemerocallis, 1-10 parts of pericarpium citri reticulatae, 1-10 parts of valeriana officinalis l, 1-5 parts of picrasma quassioides, and 1-5 parts of lophatherum gracile. The duck plague treating traditional Chinese medicine composition of the present invention has effects of heat clearing, detoxification, inflammation eliminating and diarrhea stopping, has characteristics of rapid absorbing, good treatment effect, low toxic-side effect and no drug residue, meets safe veterinary drug and animal origin food safety requirements, and is the ideal duck plague treating traditional Chinese medicine composition.

The invention provides a recombinant duck viral enteritis virus, a preparation method and applications, belonging to the field of animal medicine vaccines. The avian influenza virus haemagglutinin gene is 1437th to 3131th nucleotide with the nucleotide sequence of SEQ ID NO:1. According to the recombinant duck viral enteritis virus, an expression cassette containing the avian influenza virus haemagglutinin gene is inserted in a spacer region between LORF11 and UL55 genes of duck viral enteritis virus genome. As a vaccine active ingredient, the avian influenza virus haemagglutinin gene can achieve better cross protection effect. The recombinant duck viral enteritis virus can stably and efficiently express the avian influenza virus haemagglutinin gene, virus is stable and grows well, the prepared live vaccine has higher protective efficacy on duck plague and avian influenza, higher avian influenza antibody is generated, and the duration period of the antibody is long.

The invention discloses a double-PCR (Polymerase Chain Reaction) detection kit for newcastle disease virus and duck plague virus. A primer group provided by the invention is composed of a primer 1, a primer 2, a primer 3 and a primer 4. Nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are respectively a sequence 1, a sequence 2, a sequence 3 and a sequence 4 in a sequence table sequentially. Experiments show that newcastle disease and duck plague can be detected and identified simultaneously by one-time PCR reaction. Compared with the conventional pathogen separation identification and serological experiments, double PCRs have the characteristics of good specificity, high sensibility, fastness, simplicity, convenience, and the like, and has a high clinical application value.

The invention discloses a composition for resisting duck plague, freeze-dried powder, and a preparation method and use of the freeze-dried powder, and belongs to the technical field of veterinary feed additives. The composition comprises, by weight, 90-100 parts of a duck plague-resistant yolk antibody and 0.5-2 parts of a duck plague-resistant transfer factor. The freeze-dried powder comprises, by weight, 95-97 parts of the duck plague-resistant yolk antibody, 1-2 parts of the duck plague-resistant transfer factor, 0.2-0.3 parts of an inactivator, 0.01-0.02 parts of an antiseptic and 2.05-3.6 parts of a heat-resistant freeze-dried protective agent, and has the advantages of low preservation conditions, long preservation time, high antibody titer and good biological activity. Through combined use of the duck plague-resistant yolk antibody and the duck plague-resistant transfer factor, the freeze-dried powder has effects of preventing and treating duck plague, improves duck immunity and survival rate, is convenient for use and has a low cost.

The invention discloses a novel breeding method for cage-free ducks. The novel breeding method comprises the following steps that a duck shed is built near a pool, and a biological bed with the thickness of 52 cm is laid in the duck shed; ducklings are bred, wherein the 10-day-age ducklings are fed four times in the daytime and fed one time to two times at night, the ducklings older than 11 days of age are fed on duckling breeding feed three times in the daytime and fed on the duckling breeding feed one time at night, and the ducklings are driven into water three times to five times every day for 20 min to 30 min every time; fattening ducks are fed on fattening feed two times every day; the ducklings are inoculated with duck plague attenuated vaccines, and oxytetracycline is added into the feed at ordinary times, wherein 1 g of oxytetracycline is added into each kilogram of the feed. According to the method, the biological bed is laid in the duck shed, therefore, intestinal microbial florae of the ducks are improved, the organism immunity is enhanced, and the breeding environment is improved; padding is fermented into good organic fertilizer through piling, and the organic fertilizer is widely applied to vegetable and flower planting; the duckling breeding feed and the fattening feed are reasonable in formula and rich in nutrition, the ducks freely take food in the daytime, food is supplied by the biological bed at night, and the duck meat quality is good.

The invention provides a duck plague live vaccine and a preparation method thereof, and belongs to the technical field of biological product preparation. A chicken embryo fibroblast line DF-1 is takenas the host of duck plague viruses to produce a duck plague virus solution, and then a heatproof protective agent is added into the duck plague virus solution to prepare the duck plague live vaccine.DF-1 is used to culture duck plague viruses to avoid the safety hazard that the duck plague viruses cultured by (chicken embryo fibroblast) CEF cells are contaminated by exogenous viruses; the DF-1 cells are available at any time, time and labor are saved, and the production cost is greatly reduced. The prepared duck plague live vaccine has the advantages of good safety and high immunity effect,and can protect ducks from strong duck plague viruses. Furthermore, the live vaccine also comprises an improved heatproof protecting agent, thus the duck plague live vaccine can be stored for 24 months at a temperature of 2-8 DEG C, the virus content an titer are not reduced, the storage conditions are lowered, the storage and transportation of the live vaccine become convenient, and the production cost is reduced.

The invention relates to a pure Chinese herbal composition for treating duck plague. A preparation method of the pure Chinese herbal composition comprises the following steps: (1) separately grinding all ingredients in a prescription into superfine powders and screening by using a 700 meshes screen; (2) weighing 15-35 parts of mint, 15-25 parts of schizonepeta, 10-30 parts of fructus arctii, 15-30 parts of amur corktree bark, 10-40 of massa medicata fermentata and 10-30 parts of asarum sieboldii, and mixing for 5-10 minutes to be uniform; (3) weighing 10-30 parts of hawthorn, 15-45 parts of cirsium setosum, 10-35 parts of sanguisorba officinalis, 10-35 parts of acorus gramineus, 10-30 parts of nacre and 10-30 parts of rosewood heart wood, and mixing for 5-10 minutes to be uniform; and (4) mixing the compositions which are prepared in the steps (2) and (3), and continuously mixing for 10-15 minutes to be uniform. The pure Chinese herbal composition is reasonable in composition and simple to prepare, does not have toxic or side effects, medicinal residues or drug resistance, has high bioavailability due to adoption of superfine grinding and also has an obvious curative effect; moreover, clinical applications prove that the pure Chinese herbal composition has a special effect of treating the duck plague.

The invention discloses a compound propolis composition for treating the duck plague and a preparation method thereof, relating to the field of animal medicaments. The preparation method of the composition comprises the following steps of: (1) performing ultrafine smashing on various components (except interferon) in a formula respectively, sieving with a 700-mesh sieve; (2) weighing 15-35 parts by weight of propolis, 5-20 parts by weight of interferon for ducks, 10-30 parts by weight of cassia twig and 15-30 parts by weight of coptis root, and mixing for 5-10 minutes to uniform state; (3) weighing 10-40 parts by weight of dangshen, 10-30 parts by weight of Chinese thorowax root, 15-45 parts by weight of indigowoad leaf and 10-35 parts by weight of red paeony root, and mixing for 5-10 minutes to a uniform state; and (4) combining the compositions obtained in the steps (2) and (3), and continually mixing for 10-15 minutes to uniform state to obtain the compound propolis composition. The preparation method has the beneficial effects of reasonable formula and easiness for preparing; the compound propolis composition has the advantages of no toxic or side effect, environmental friendliness, no medicament residue, no medicament tolerance, ultrafine smashing, high bioavailability and very remarkable effect; and as proved by clinical application, the compound propolis composition has a special treatment effect on the duck plague.

The invention discloses a special antiviral traditional Chinese medicine preparation capable of increasing feed intake for laying ducks. The special antiviral traditional Chinese medicine preparation is prepared from the following raw material components: honeysuckles, fructus forsythia, dried rehamnnia root, moutan bark, root of common peony, hawthorn, malt, codonopsis pilosula, rheum officinale, glossy privet fruit, polygonum multiflorum, radix curcumae, reed rhizome, polygonum cuspidatum, scutellaria baicalensis, indigo naturalis, medicated leaven, pumpkin seed, Chinese yam and hyacinth bean. The special antiviral traditional Chinese medicine preparation capable of increasing feed intake for laying ducks has the effects of clearing away heat and toxic materials and achieving antisepsis and anti-inflammation, can be used for treating viral diseases of duck plague, duck virus hepatitis and the like, can be used for increasing the feed intake of the laying ducks and achieving treating both manifestation and root cause of disease, is quick to become effective, good in curative effect, free of toxic and side effect, free of interferences of the egg yield of the laying ducks and low in cost, so that the economic income of a farmer is increased.

The invention provides a traditional Chinese medicine composition for preventing and treating duck plague and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 25-38 parts of humifuse euphorbia herb, 25-38 parts of herba portulacae, 20-32 parts of radix bupleuri, 20-30 parts of radix isatidis, 15-28 parts of cassia twigs, 15-28 parts of folium isatidis, 13-25 parts of agastache rugosa, 10-22 parts of dandelions, 10-20 parts of ligusticum wallichii, 8-15 parts of fructus aurantii and 8-15 parts of licorice roots. The traditional Chinese herbal medicinal materials are combined to achieve the effects of clearing heat, removing toxicity, cooling blood, stopping bleeding, harmonizing the exterior and interior and relieving diarrhea and pain, has remarkable effects on preventing and treating duck plague, has the advantages of no drug residue, no drug resistance and low cost, and is environment-friendly and safe.

The invention relates to duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague, which comprises each following component according to weight percent: 30 to 60 percent of egg yolk antibody for duck plague, 30 to 55 percent of egg yolk antibody for duck virus hepatitis, 0.2 to 0.3 percent of formaldehyde, 0.01 to 0.02 percent of thiomersalate, 4 to 5 percent of glucose, 1 to 2 percent of sorbitol, 2 to 3 percent of glycocoll and 2 to 3 percent of mannitol. The invention discloses a preparation method of the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague simultaneously, which comprises the following steps of: separating a strain, preparing a vaccine, immunizing a healthy duck flock, preparing eggs, carrying out acidizing treatment, octoate treatment and refining, extracting and purifying treatment after separating egg yolks, preparing and freeze-drying so as to obtain the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague. According to the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague and the preparation method thereof, viruses sampled in regions with high morbidity are separated, the virus strain with wider coverage range is sieved, the vaccine is prepared, the cold / hot acidizing treatment of an egg yolk solution is also carried out, the antibody loss is reduced, and the obtained egg yolk antibody freeze-drying powder has high recovery rate and high purity, can dissolve and act quickly and has no clinical side effect.

The invention relates to an application of thymopentin in an intranasal vaccine, which is characterized in that the thymopentin is added in the existing intranasal vaccine as an adjuvant for improving immune response capability of an animal to the exiting intranasal vaccine. Compared with the prior art, the thymopentin is adopted as the immune adjuvant, thereby being capable of greatly improving antibody titer generated after the animal is immunized with the intranasal vaccine and also prolonging high antibody titer level generated after the animal is immunized with the vaccine; the range of animal objects using the immunization and the range of varieties of the intranasal vaccines are wide; and immunization can be completed by one-time injection after being compatible with the intranasal vaccine, and the using method is simple. Even in regions where highly pathogenic blue ear disease, circovirus, newcastle disease, duck plague and other epidemic diseases capable of damaging immune systems of animal bodies are endemic, the nationally stipulated standard can be achieved after the use of the immunization, the protection rate of a colony can be above 70%, and the animal colony can be effectively protected.

The invention discloses a duck plague virus recombinant vaccine strain expressing an enhanced green fluorescent protein (EGFP) gene, a constructing method thereof and applications of the recombinant vaccine strain. In particular, by utilization of a recombinant clone technology, a gene fragment CMV-EGFP containing an enhanced green fluorescent protein (EGFP) and a CMV promoter sequence replaces a US10 gene of a duck plague virus, and a recombinant EGFP duck plague virus lacking the US10 gene with a CMV-EGFP expression cassette being inserted into the corresponding position is constructed. The recombinant virus can stably express the EGFP gene. The duck plague virus recombinant vaccine strain is named as rDEVUS10-EGFP. The microbial accession number is CGMCC No.8660. The invention also relates to constructing methods of duck plague virus recombinant vaccine strains capable of stably expressing other poultry pathogeny exogenous genes, and applications of the recombinant vaccine strains in preparation of vaccines preventing duck plague and other poultry infectious diseases.

The invention discloses a duck viral hepatitis and duck plague bigeminal egg yolk antibody oral solution and a preparation method thereof. The formula is characterized in that the duck viral hepatitis and duck plague bigeminal egg yolk antibody oral solution comprises the following raw materials in percentage by volume: 95-105 of a whole-egg water solution containing an egg yolk antibody, 0.2-0.3 of octanoic acid, 0.2-0.3 of formaldehyde, 0.01-0.02 of merthiolate and 800-1000 units of mycillin in every milliliter of the oral solution. Whole eggs with high immunoglobulin are adopted during preparation, so that the clear egg yolk antibody oral solution is obtained. The egg yolk antibody oral solution prepared by using the preparation method is not only low in loss, high in immune active substance content, high in biological activity and free of clinical side effects in the production process, but also contains a great number of various proteins in egg white, so that the adverse effects on the antibody can be reduced during oral administration.