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Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof

A yolk antibody and infectivity technology, which is applied in the field of mixed yolk antibody for prevention and treatment of infectious serositis in ducks and its preparation, can solve the problem that the research on yolk antibody of R. anatipestifer has not yet been reported, and can reduce the use of antibiotics , high protection rate, and the effect of promoting development

Active Publication Date: 2009-07-08
兆丰华生物科技(南京)有限公司北京生物医药科技中心 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most viral diseases, such as Newcastle disease, egg drop syndrome, chicken infectious bursal disease, duck viral hepatitis, etc., have successfully developed yolk antibodies, but the research on yolk antibodies of R. No report

Method used

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  • Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Riemerella anatipestifer egg yolk antibody

[0031] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0032] Riemerella anatipestifer RA1 (serotype I) and RA2 (serotype II) strains were respectively inoculated into tryptone yeast broth containing 0.5% serum, and cultured at 36~37℃ with shaking for 18 hours to logarithmic growth phase Centrifuge at 3000 rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, and slowly add the analytical pure formaldehyde solution to 0.3% of the total bacterial solution, at 36~37 Inactivate for 36 hours at a constant temperature of ℃;

[0033] (2) Mix the antigen in proportion and add the immune adjuvant;

[0034] Mix the inactivated RA1 and RA2 bacterial antigens in a ratio of 1:2, add the sterilized complete Freund's adjuvant to some equal parts, and add the incomplete Freund's adjuvan...

Embodiment 2

[0040] Example 2 Preparation of Riemerella anatipestifer egg yolk antibody

[0041] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0042] Take Riemerella anatipestifer (serotype I) and RA2 (serotype II) strains, respectively, inoculate them in tryptone yeast broth containing 0.5% serum, and culture at 36~37℃ for 18 hours to the logarithmic growth phase. Centrifuge at 3000 rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, and slowly add the analytical pure formaldehyde solution to 0.3% of the total bacterial solution. Inactivate for 36 hours at a constant temperature of ~37℃;

[0043] (2) Mix the antigens in proportion and add immune adjuvants;

[0044] After inactivation, mix RA1 and RA2 bacterial antigens in a ratio of 1:4, add sterilized propolis in equal volume, and emulsify to obtain bacterial antigens;

[0045](3) Choose healthy non-immu...

Embodiment 3

[0050] Example 3 Preparation of Riemerella anatipestifer egg yolk antibody

[0051] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0052] Take Riemerella anatipestifer (serotype I) and RA2 (serotype II) strains, respectively inoculate them in tryptone yeast broth containing 0.5% serum, and culture at 36~37℃ for 18 hours to logarithmic growth phase with shaking Centrifuge at 3000 rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, and slowly add the analytical pure formaldehyde solution to 0.3% of the total bacterial solution, at 36~37 Inactivate for 36 hours at a constant temperature of ℃;

[0053] (2) Mix the antigen in proportion and add the immune adjuvant;

[0054] After inactivation, mix the RA1 and RA2 bacterial antigens in a ratio of 1:6, add the sterilized white oil for injection in equal volume, and emulsify the bacterial antigens;

[...

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Abstract

The invention relates to a mixed yolk antibody for preventing and controlling infective oromeningitis and a preparation method thereof, and belongs to the technical field of biological agent preparation and poultry disease prevention and control. The invention takes a main epidemic serotype strain of duck plague pasteurella anatipestifer as an antigen to prepare the duck plague pasteurella anatipestifer hyperimmune yolk antibody. Infection treatment experimental results show that the prepared yolk antibody has the protection ratio for serotype I and serotype II duck plague pasteurella anatipestifer respectively reaching 80 percent and 90 percent. The product provides a new way to the prevention and control of duck infective oromeningitis and can be used for the preparation of veterinary medicines for controlling the disease or be prepared into health care products or feed additives for use. Moreover, the yolk antibody provides a material basis for improving the livestock product quality, reducing medicinal residue and promoting the human food safety.

Description

Technical field [0001] The invention belongs to the technical field of biological preparations and poultry disease prevention and control, and relates to a mixed yolk antibody for preventing and treating duck infectious serositis and a preparation method thereof. Background technique [0002] Riemerella anatipestifer (RA) disease, also known as infectious serositis of ducks, is currently one of the most serious infectious diseases harmful to the duck industry. It often causes ducklings, goslings, turkeys, etc. Fibrinous pericarditis, perihepatitis, air sacculitis, caseous salpingitis, conjunctivitis, arthritis, etc. of breeders have high morbidity and mortality. The disease is caused by Riemerella anatipestifer, which is sensitive to some antibacterial drugs, but it is difficult to cure, especially after the bacteria invades the air sacs, the drugs are difficult to reach, and it is more difficult to remove them. At present, due to unreasonable medication regimens, R. anatipestife...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/02C07K1/14A61K9/08A61K39/40A61K39/39A23K1/16A61P31/04A23K10/20A23K20/147A23K40/00A23K50/75
Inventor 陈义锋赵亚荣张渊魁赵建增
Owner 兆丰华生物科技(南京)有限公司北京生物医药科技中心
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