35results about How to "Solve prevention and control problems" patented technology

The invention discloses an environment-friendly mining design method for mine mining, screening, pressure relief, drainage and filling. The environment-friendly mining design method includes the stepsthat firstly, a protective layer on total rock is mined to increase an interlayer crack between the protective layer and a protected layer, and gas pressure relief of the protected layer is realized;meanwhile, high gangue content raw coal produced by mining of the protective layer is transported to an underground coal gangue washing system, and washed gangue is stored in a gangue silo; a gas drainage system is then arranged in the protective layer and the protected layer, and safe and efficient co-mining of a coal seam with low permeability and high gas is realized; and finally, the gangue stored in the gangue silo is transported to the ultra-long mixed mining working face of the protective layer to fill a goaf. According to the environment-friendly mining design method, the difficult problem of gas prevention of the coal seam with the low permeability and the high gas is effectively solved, environment pollution and damage caused by gangue ground discharging are avoided, the miningrate of mine resources and the recovery rate of coal-bearing associated resources are improved, the application range of a protective layer mining technology and a solid filling coal mining method isbroadened, and important technical reference can be provided for mining mines with the same conditions.

The invention discloses a bacterial strain and a culture method and application thereof. The bacterial strain is Pseudomonas fluorescens and is collected under the number of CGMCC No.10730. A culture medium of the bacterial strain is prepared with, by mass, 0.1-0.2% of yeast extracct, 0.5-0.6% of peptone, 0.3-0.4% of beef extract, 1-2% of sucrose and the balance of distilled water. The Pseudomonas fluorescens Sneb825 or a metabolite thereof has inducing activity to tomatoes, can be used for seed treatment of the tomatoes, can promote growth of tomato plants and roots and has great inhibition effect on root-knot forming of root-knot nematodes, and the problem of prevention and control of the root-knot nematodes is solved.

The invention discloses a method for controlling root knot nematode by utilizing ozone water. In the method, the ozone water is used as root irrigation physic liquor, and a plant root zone is irrigated once per 5-15 days until fruits are harvested. By utilizing the ozone water to continuously carry out root irrigation on plant soils for many times, the generation of the root knot nematode can be efficiently controlled, damage on the growth of plants can not be caused either, and the method is a new technology of green food production.

The invention discloses a novel bacteria strain and a culture method and application thereof. The bacteria strain is pseudomonas putida with the preservation number of CGMCC No. 10729; the culture medium of the novel bacteria strain is prepared from the following raw materials in percentage by mass: 0.1-0.2% of a yeast extract, 0.5-0.6% of peptone, 0.3-0.4% of a beef extract and 1-2% of sucrose, pseudomonas putida (Sneb 821) or metabolite thereof has an activity of inducing tomatoes, can be used for seed treatment of tomatoes, can not only promote the growth of tomato plants and roots, but also has a great inhibiting effect on formation of meloidogyne incognita, and further solves the problem of prevention and treatment of root-knot nematodes.

The invention discloses a compound fertilizer for amaranth. The fertilizer contains the following raw materials in parts by weight: 20-30 parts of cotton straws, 7-12 parts of waste glass, 10-30 parts of animal feces, 12-22 parts of a phosphatic fertilizer, 8-17 parts of a potash fertilizer, 15-30 parts of oil plant cakes, 2-7 parts of polyacrylamide, 1-2 parts of borax, 0.6-1.1 parts of ammonium molybdate, 2-3 parts of zeolite powder, 5-10 parts of montmorillonite, 1-3 parts of fermented microorganisms, 1-2 parts of an enveloping agent, 2-3 parts of an anti-worm substance and 1-2 parts of a deodorant. The compound fertilizer for amaranth is convenient to use, increases both yield and incoming, favorably improves the utilization ratio of nutrients and can improve the physical and chemical properties of soil.

Fertilizer with insect expelling and antibacterial functions is prepared from main nutritious components in percentage by mass as follows: 15%-25% of nitrogen, 5%-15% of phosphorus and 10%-20% of potassium, and the fertilizer further comprises an insect expelling substance with the weight accounting for 3%-7% of the total weight of the fertilizer. The insect expelling substance is prepared from, in percentage by mass, 8%-12% of lycorine, 75%-85% of bamboo vinegar, 2%-7% of sodium lignin sulfonate, 2%-7% of tobacco powder and 3%-8% of polyethylene glycol through mixing. The fertilizer has the benefits as follows: active ingredients including lycorine and the tobacco powder with strong insect expelling activity are taken as the main insect expelling ingredients, the bamboo vinegar can inhibit growth of weeds when used for preparing the fertilizer, the dosage of pesticides can be reduced, the compound fertilizer has excellent insect expelling and hazard avoiding functions, sodium lignin sulfonate is used as a surfactant, all substances can be effectively and evenly mixed by use of polyethylene glycol, and the fertilizer efficiency and the insect expelling effect of the fertilizer are improved.

The invention discloses pseudomonas fragi Sned811 with a function of killing tomato southern root-knot nematode with poison. A classification name of the pseudomonas fragi Sned811 is Pseudomonas fragi, the pseudomonas fragi Sned811 is preserved in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms on April 12th, 2017, and a preservation number of the pseudomonas fragi Sned811 is CGMCC No.14018. The pseudomonas fragi Sned811 has the advantages that biological preparations with strong effects of killing the tomato southern root-knot nematode with poison can be developed from a metabolite obtained after a strain of the pseudomonas fragi Sned811 is cultured according to conventional liquid fermentation culture, the strong effects of killing the tomato southern root-knot nematode with poison can be realized, obvious effects of inhibiting hatching of oocysts of the tomato southern root-knot nematode can be realized, and accordingly the pseudomonas fragi Sned811 is a biological control bacterium with great potential and has an excellent application prospect in the aspect of controlling harm on crops due to root-knot nematode.

The invention provides a preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus, which belongs to the technical field of biological control for forest pests. The preparation method is characterized by comprising the following concrete steps: 1) carrying out inoculated culture on Beauveria bassiana strains so as to obtain fermentation broth; 2) separating the fermentation broth, collecting supernatant and removing mycelia so as to obtain a filtrate which is a crude extracellular holoprotein extract; 3) adding ethylene diamine tetraacetic acid into the crude extracellular holoprotein extract, adding ammonium sulfate powder under an ice bath condition, carrying out stirring and dissolving, standing an obtained mixture overnight and dissolving an obtained precipitate with a phosphate buffer solution so as to obtain a concentrate of the crude extracellular holoprotein extract; and 4) carrying out dialysis on the crude extracellular holoprotein extract, separating the concentrate and carrying out filtering and sterilization on obtained supernatant so as to obtain the extracellular holoprotein extract. The extracellular holoprotein extract prepared by using the preparation method provided in the invention has the advantages of strong insecticidal activity, capacity of controlling Monochamus alternatus, safety and high efficiency in usage, no pollution and a good control effect and is a good biological control reagent.

The invention relates to construction of rough type brucella of a recombinant fine-granule echinococcus Eg95 gene and a vaccine of the rough type Brucella. A rough type cattle-species Brucella low virulent strain RA343 is used as a parent strain, cane sugar suicide plasmid is used as a carrier, the fine-granule echinococcus Eg95 gene containing a specific promoter sequence is optimized through a codon, then the optimized fine-granule echinococcus Eg95 gene is inserted in a brucella genome in a traceless manner, and a recombinant brucella RA343-Eg95 strain which can efficiently express the recombinant fine-graunle echinococcus Eg95 gene is successfully constructed. The recombinant strain not only reserves rough type characteristics of an original parent strain RA343 and has favorable immunoprotection properties on brucellemia (brucellosis); and besides, after animals are immunized with the recombinant strain, antibodies in accordance with fine-granule echinococcus Eg95 can be generated,so that immunoprotection for echinococcosis granulosa can be realized. After the animals are immunized with the vaccine prepared from the recombinant strain, double immunoprotection for the brucellosis and the echinococcosis granulosa can be realized at the same time.

The invention provides a yellow peach tree culturing fertilizer with insect-expelling anti-bacterial functions and a preparation method thereof. The yellow peach tree culturing fertilizer comprises the following components in parts by weight: 20-25 parts of dry animal waste, 20-22 parts of tobacco straws, 8-10 parts of bamboo vinegar, 6-9 parts of lycorine, 3-5 parts of monopotassium phosphate, 2-4 parts of bacterial manure and 3-5 parts of urea. The invention aims to solve the problems that the present yellow peach yield and quality cannot meet the practical requirement and the plant diseases and insect pests are serious under the condition of no pesticide application. The invention belongs to the field of yellow peach planting.

The invention discloses a breeding method of a herbicide-resistant rice two-line sterile line. According to the breeding method, an imidazolone-herbicide-resistant rice mutant is taken as a resistantgene donor parent, an excellent two-line sterile line is taken as a recurrent parent, continuous backcross is adopted so as culture the herbicide-resistant rice two-line sterile line which is excellent in agricultural properties, possesses sterility highly consistent to that of the recurrent parent and herbicide-resistant genes; the matching success rate of excellent hybridization rice combinations is increased, excellent and high yield herbicide-resistant two-line hybridization rice is cultured, the cultured herbicide-resistant two-line hybridization rice, routine rice varieties and the recurrent parent are subjected to matching, the hybridization later generations all possesses herbicide resistance, and weed control difficult problems in rice fields are solved effectively.

The invention provides a discharge outlet purification system and a purification method thereof. The purification system is carried at a discharge outlet of sewage, the sewage is discharged after being purified by the discharge outlet purification system, and the discharge outlet purification system comprises a dosing section, a pretreatment section, a filler treatment section, a biochemical treatment section and a filter membrane treatment section. According to the system, the space of the receiving water area of the discharge outlet is transformed, purification treatment of discharged waterof the discharge port is achieved, and the system has the advantages of being high in applicability, rapid, efficient, multifunctional, flexible in operation and the like.

The invention relates to construction of a rough Brucella strain of a recombinant chlamydia psittaci outer membrane protein MOMP gene and a vaccine thereof. A rough bovine Brucella attenuated strain RA343 is taken as a parent strain, a sucrose suicide plasmid is taken as a vector, the Chlamydia psittaci outer membrane protein MOMP gene containing a specific promoter sequence is inserted into a Brucella genome without trace after codon optimization, and the recombinant Brucella strain RA343-MOMP capable of efficiently expressing the Chlamydia psittaci outer membrane protein gene is successfullyconstructed. The recombinant strain not only retains the rough type characteristic of an original parent strain RA343 and has good immunoprotection on brucellosis (brucellosis). In addition, an MOMPantibody against chlamydia psittaci can be generated after the recombinant strain is used for immunizing animals, and accordingly immunoprotection on chlamydia psittaci is achieved. The vaccine prepared by the recombinant vaccine strain can simultaneously realize double immune protection on brucellosis and chlamydia psittaci after being used for immunizing animals.

The invention discloses a pest repellent containing a compound microbial agent as well as a preparation method and application of the pest repellent and belongs to the technical field of pest prevention and control. The pest repellent is prepared from the following raw materials in parts by weight: 5-20 parts of compound microorganisms, 1.5-5 parts of pyrethrum, 1-5 parts of marigold, 1-2 parts ofdaphne odera, 0.1-0.8 part of folium artemisiae argyi, 0.06-0.15 part of garland chrysanthemum, 1-2 parts of radix stemonae, 0.2-0.65 part of celastrus angulatus, 1-1.5 parts of catmint, 0.1-0.5 partof wood vinegar, 3-4 parts of rice vinegar, 8-15 parts of brown sugar, 6-15 parts of wine, 0.2-0.5 part of sorbitol, 2-9 parts of lycorine and 40-50 parts of deionized water. The pest repellent disclosed by the invention contains a microbial preparation, so that the present prevention and control problems of underground pests are solved; and a microbial fermentation technology is combined, so that the pest repellent is free of chemical residues, safe and efficient in use and incapable of polluting the environment.

The invention discloses a method for preventing and treating brassicaceous vegetable leaf beetle type pests through rumex weeds, and mainly solves the problems that due to the adoption of chemical pesticide for treating the brassicaceous vegetable leaf beetle type pests, brassicaceous vegetables are easy to pollute and the treating effect is poor. According to the method for preventing and treating the brassicaceous vegetable leaf beetle type pests through the rumex weeds, the rumex weeds are used for trapping and killing the leaf beetle type pests, so that the vegetables are difficult to pollute, and the treating effect is good.

The invention provides an in-advance insect prevention method for efficiently controlling a strawberry virus disease. The method includes the step: performing insect prevention treatment on strawberries at an early stage of planting of the strawberries to cut off a transmission route of the strawberry virus disease. An insect prevention treatment mode includes insecticide spraying and soil disinfection. Controlled injurious insects include thrips, aphids, whiteflies, spider mites and nematodes in the insect prevention treatment process. The strawberry virus disease is transmitted by mouthpartsof the injurious insects such as the thrips and the aphids or by the nematodes through soil. The strawberry virus disease is effectively controlled by the in-advance insect prevention method, 'the transmission route' of the strawberry virus disease is effectively cut off, namely, the strawberry virus disease is controlled from the source, the control efficiency of the strawberry virus disease ishigh, and control effects are remarkable.

The invention relates to construction of rough type brucella of a recombinant fine-granule echinococcus Eg95 gene and a vaccine of the rough type Brucella. A rough type cattle-species Brucella low virulent strain RA343 is used as a parent strain, cane sugar suicide plasmid is used as a carrier, the fine-granule echinococcus Eg95 gene containing a specific promoter sequence is optimized through a codon, then the optimized fine-granule echinococcus Eg95 gene is inserted in a brucella genome in a traceless manner, and a recombinant brucella RA343-Eg95 strain which can efficiently express the recombinant fine-graunle echinococcus Eg95 gene is successfully constructed. The recombinant strain not only reserves rough type characteristics of an original parent strain RA343 and has favorable immunoprotection properties on brucellemia (brucellosis); and besides, after animals are immunized with the recombinant strain, antibodies in accordance with fine-granule echinococcus Eg95 can be generated,so that immunoprotection for echinococcosis granulosa can be realized. After the animals are immunized with the vaccine prepared from the recombinant strain, double immunoprotection for the brucellosis and the echinococcosis granulosa can be realized at the same time.

The invention relates to a mixed yolk antibody for preventing and controlling infective oromeningitis and a preparation method thereof, and belongs to the technical field of biological agent preparation and poultry disease prevention and control. The invention takes a main epidemic serotype strain of duck plague pasteurella anatipestifer as an antigen to prepare the duck plague pasteurella anatipestifer hyperimmune yolk antibody. Infection treatment experimental results show that the prepared yolk antibody has the protection ratio for serotype I and serotype II duck plague pasteurella anatipestifer respectively reaching 80 percent and 90 percent. The product provides a new way to the prevention and control of duck infective oromeningitis and can be used for the preparation of veterinary medicines for controlling the disease or be prepared into health care products or feed additives for use. Moreover, the yolk antibody provides a material basis for improving the livestock product quality, reducing medicinal residue and promoting the human food safety.

The invention relates to a pesticide fertilizer composite containing fipronil and imidaclothiz specially for sugarcane and application of the pesticide fertilizer composite and belongs to the technical field of agricultural chemistry. The pesticide fertilizer composite includes pesticide components, fertilizer components and addition agents, wherein the pesticide components consist of fipronil andimidaclothiz, and the fertilizer components include iron fertilizer, manganese fertilizer, copper fertilizer, zinc fertilizer, boric fertilizer and molybdenum fertilizer which are demanded in the growth of the sugarcane; the addition agents include a dispersing agent, a wetting agent and a filler, wherein the mass ratio of the pesticide components to the fertilizer components to the addition agents is (1-3):(80-85):(5-18), and the mass ratio of fipronil to imidaclothiz in the pesticide components is (1-3):(3-1). The pesticide fertilizer composite can not only supplement trace elements essential for the sugarcane, promote the growth of the sugarcane and increase the yield of the sugarcane but also can prevent pests on sugarcane, wherein the prevention effect on sugarcane moth borers reaches 80% or above, the prevention effect on scarabs reaches 83% or above, and the prevention effect on woolly aphids reaches 95%. The pesticide fertilizer composite is applied conveniently and is conducive to application and popularization.

The invention relates to a sugarcane special insecticide composition containing fipronil and imidaclothiz and an application of the composition, and belongs to the technical field of agricultural chemistry. According to the sugarcane special insecticide composition containing the fipronil and the imidaclothiz, the weight ratio of the fipronil to the imidaclothiz is (1-3):(3-1). In order to facilitate production and application, the insecticide composition can be prepared into various dosage forms and is preferably prepared into wettable powder which comprise the insecticide composition and anadditive, the additive comprises a dispersing agent, a wetting agent and a filler, and the content of the insecticide composition is 10%-70%. The insecticide composition, large sugarcane cultivated soil and fertilizers can be combined and integrated applied, the control effect on sugarcane borer strains reaches 85% or more, the control effect on sugarcane borers reaches 90% or more, the control effect on scarabs reaches 85% or more, the control effect on woolly aphids reaches 955 or more, and the sugarcane special insecticide composition is high in insect killing efficiency and good in controleffect. A chemical of the insecticide composition is convenient to apply, and popularization and application are facilitated.

The invention discloses a wheat planting method and belongs to the technical field of wheat planting. The method is used for solving the problem of low wheat yield in the prior art and comprises the steps of S1, land preparation, wherein pretreatment is conducted on a wheat field, deep ploughing in the depth of 22-30 cm is carried out on soil, a herbicide is sprayed onto the ploughed soil, insolation is carried out for 2-5 days in the sun, and then a mixture of plant ash and limestone is laid in the wheat field; S2, seed selection, wherein seeds are soaked in a seed selection solution for seedselection, then washed with clean water after the seed selection and soaked in a potassium permanganate solution with the concentration of 0.3-0.5% for 24-36 hours, and then the seeds are conveyed into a potassium dihydrogen phosphate solution with the concentration of 0.4-0.5% to be soaked for 12-24 hours after being air-dried completely; S3, sowing, wherein sowing is carried out row by row, thesowing depth is 10-15 cm, the sowing row spacing is 10-20 cm, 2-5 kg of the seeds are sown per mu, and 15-18 kg of base fertilizer is applied per mu; S4, fertilization, wherein the growth condition of wheat seedlings is regularly measured, and compound fertilizer is applied to the wheat field; S5, harvesting, wherein wheat is harvested mechanically in a wheat ripening season.

The invention provides a duck reovirus SL strain; the strain is collected in China Center for Type Culture Collection in Wuhan University; the collection number is CCTCC No:V201970, and the inventionaims to solve the technical problem that traditional Chinese veterinary drugs in the prior art have no obvious effect on prevention and treatment of the duck reovirus. The use of an antigen-antibody complex of the duck reovirus can solve the difficult problem of clinical prevention and control of the duck reovirus, promotes income increasing for breeders, escorts healthy development of the duck breeding industry in China, and has remarkable economic and social benefits.

A composition for biological control of capsicum blight, its preparation method and application. The composition mainly includes the following components: green wood enzyme preparation, Paecilomyces lilacinus preparation, Streptomyces scab preparation and organic materials. The preparation method is to stir and react each component at a speed of 30-50 r / min at normal temperature for 5-7 minutes. The invention also discloses the application of the composition for biological control of capsicum blight. The composition of the invention can eradicate the occurrence of capsicum blight, increase yield, improve soil organic matter, fertilize soil fertility, be environmentally friendly, low cost, and realize sustainable utilization of soil. The method and application of the invention are simple and suitable for agricultural production.

The invention provides a preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus, which belongs to the technical field of biological control for forest pests. The preparation method is characterized by comprising the following concrete steps: 1) carrying out inoculated culture on Beauveria bassiana strains so as to obtain fermentation broth; 2) separating the fermentation broth, collecting supernatant and removing mycelia so as to obtain a filtrate which is a crude extracellular holoprotein extract; 3) adding ethylene diamine tetraacetic acid into the crude extracellular holoprotein extract, adding ammonium sulfate powder under an ice bath condition, carrying out stirring and dissolving, standing an obtained mixture overnight and dissolving an obtained precipitate with a phosphate buffer solution so as to obtain a concentrate of the crude extracellular holoprotein extract; and 4) carrying out dialysis on the crude extracellular holoprotein extract, separating the concentrate and carrying out filtering and sterilization on obtained supernatant so as to obtain the extracellular holoprotein extract. The extracellular holoprotein extract prepared by using the preparation method provided in the invention has the advantages of strong insecticidal activity, capacity of controlling Monochamus alternatus, safety and high efficiency in usage, no pollution and a good control effect and is a good biological control reagent.

The invention provides a cultivation method of selenium-enriched Kiwi berry, and relates to the technical field of agricultural production. The method comprises the following steps of (1) cultivationof seedling; (2) field planting; (3) pollination, (4) fertilization management and (5) disease control. Via reasonable fertilizers and plantation, the Kiwi berry is high in survival rate and growth quality, fertilizing is reasonable so that a lot of natural organic selenium protein elements are generated in the Kiwi berry plant bodies, the selenium-enriched Kiwi berry is produced, and the method is suitable for large scale plantation.