35results about How to "Solve prevention and control problems" patented technology

The invention discloses an environment-friendly mining design method for mine mining, screening, pressure relief, drainage and filling. The environment-friendly mining design method includes the stepsthat firstly, a protective layer on total rock is mined to increase an interlayer crack between the protective layer and a protected layer, and gas pressure relief of the protected layer is realized;meanwhile, high gangue content raw coal produced by mining of the protective layer is transported to an underground coal gangue washing system, and washed gangue is stored in a gangue silo; a gas drainage system is then arranged in the protective layer and the protected layer, and safe and efficient co-mining of a coal seam with low permeability and high gas is realized; and finally, the gangue stored in the gangue silo is transported to the ultra-long mixed mining working face of the protective layer to fill a goaf. According to the environment-friendly mining design method, the difficult problem of gas prevention of the coal seam with the low permeability and the high gas is effectively solved, environment pollution and damage caused by gangue ground discharging are avoided, the miningrate of mine resources and the recovery rate of coal-bearing associated resources are improved, the application range of a protective layer mining technology and a solid filling coal mining method isbroadened, and important technical reference can be provided for mining mines with the same conditions.

The invention discloses a novel bacteria strain and a culture method and application thereof. The bacteria strain is pseudomonas putida with the preservation number of CGMCC No. 10729; the culture medium of the novel bacteria strain is prepared from the following raw materials in percentage by mass: 0.1-0.2% of a yeast extract, 0.5-0.6% of peptone, 0.3-0.4% of a beef extract and 1-2% of sucrose, pseudomonas putida (Sneb 821) or metabolite thereof has an activity of inducing tomatoes, can be used for seed treatment of tomatoes, can not only promote the growth of tomato plants and roots, but also has a great inhibiting effect on formation of meloidogyne incognita, and further solves the problem of prevention and treatment of root-knot nematodes.

The invention discloses a compound fertilizer for amaranth. The fertilizer contains the following raw materials in parts by weight: 20-30 parts of cotton straws, 7-12 parts of waste glass, 10-30 parts of animal feces, 12-22 parts of a phosphatic fertilizer, 8-17 parts of a potash fertilizer, 15-30 parts of oil plant cakes, 2-7 parts of polyacrylamide, 1-2 parts of borax, 0.6-1.1 parts of ammonium molybdate, 2-3 parts of zeolite powder, 5-10 parts of montmorillonite, 1-3 parts of fermented microorganisms, 1-2 parts of an enveloping agent, 2-3 parts of an anti-worm substance and 1-2 parts of a deodorant. The compound fertilizer for amaranth is convenient to use, increases both yield and incoming, favorably improves the utilization ratio of nutrients and can improve the physical and chemical properties of soil.

The invention discloses pseudomonas fragi Sned811 with a function of killing tomato southern root-knot nematode with poison. A classification name of the pseudomonas fragi Sned811 is Pseudomonas fragi, the pseudomonas fragi Sned811 is preserved in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms on April 12th, 2017, and a preservation number of the pseudomonas fragi Sned811 is CGMCC No.14018. The pseudomonas fragi Sned811 has the advantages that biological preparations with strong effects of killing the tomato southern root-knot nematode with poison can be developed from a metabolite obtained after a strain of the pseudomonas fragi Sned811 is cultured according to conventional liquid fermentation culture, the strong effects of killing the tomato southern root-knot nematode with poison can be realized, obvious effects of inhibiting hatching of oocysts of the tomato southern root-knot nematode can be realized, and accordingly the pseudomonas fragi Sned811 is a biological control bacterium with great potential and has an excellent application prospect in the aspect of controlling harm on crops due to root-knot nematode.

The invention provides a preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus, which belongs to the technical field of biological control for forest pests. The preparation method is characterized by comprising the following concrete steps: 1) carrying out inoculated culture on Beauveria bassiana strains so as to obtain fermentation broth; 2) separating the fermentation broth, collecting supernatant and removing mycelia so as to obtain a filtrate which is a crude extracellular holoprotein extract; 3) adding ethylene diamine tetraacetic acid into the crude extracellular holoprotein extract, adding ammonium sulfate powder under an ice bath condition, carrying out stirring and dissolving, standing an obtained mixture overnight and dissolving an obtained precipitate with a phosphate buffer solution so as to obtain a concentrate of the crude extracellular holoprotein extract; and 4) carrying out dialysis on the crude extracellular holoprotein extract, separating the concentrate and carrying out filtering and sterilization on obtained supernatant so as to obtain the extracellular holoprotein extract. The extracellular holoprotein extract prepared by using the preparation method provided in the invention has the advantages of strong insecticidal activity, capacity of controlling Monochamus alternatus, safety and high efficiency in usage, no pollution and a good control effect and is a good biological control reagent.

The invention relates to construction of rough type brucella of a recombinant fine-granule echinococcus Eg95 gene and a vaccine of the rough type Brucella. A rough type cattle-species Brucella low virulent strain RA343 is used as a parent strain, cane sugar suicide plasmid is used as a carrier, the fine-granule echinococcus Eg95 gene containing a specific promoter sequence is optimized through a codon, then the optimized fine-granule echinococcus Eg95 gene is inserted in a brucella genome in a traceless manner, and a recombinant brucella RA343-Eg95 strain which can efficiently express the recombinant fine-graunle echinococcus Eg95 gene is successfully constructed. The recombinant strain not only reserves rough type characteristics of an original parent strain RA343 and has favorable immunoprotection properties on brucellemia (brucellosis); and besides, after animals are immunized with the recombinant strain, antibodies in accordance with fine-granule echinococcus Eg95 can be generated,so that immunoprotection for echinococcosis granulosa can be realized. After the animals are immunized with the vaccine prepared from the recombinant strain, double immunoprotection for the brucellosis and the echinococcosis granulosa can be realized at the same time.

The invention provides a yellow peach tree culturing fertilizer with insect-expelling anti-bacterial functions and a preparation method thereof. The yellow peach tree culturing fertilizer comprises the following components in parts by weight: 20-25 parts of dry animal waste, 20-22 parts of tobacco straws, 8-10 parts of bamboo vinegar, 6-9 parts of lycorine, 3-5 parts of monopotassium phosphate, 2-4 parts of bacterial manure and 3-5 parts of urea. The invention aims to solve the problems that the present yellow peach yield and quality cannot meet the practical requirement and the plant diseases and insect pests are serious under the condition of no pesticide application. The invention belongs to the field of yellow peach planting.

The invention discloses a breeding method of a herbicide-resistant rice two-line sterile line. According to the breeding method, an imidazolone-herbicide-resistant rice mutant is taken as a resistantgene donor parent, an excellent two-line sterile line is taken as a recurrent parent, continuous backcross is adopted so as culture the herbicide-resistant rice two-line sterile line which is excellent in agricultural properties, possesses sterility highly consistent to that of the recurrent parent and herbicide-resistant genes; the matching success rate of excellent hybridization rice combinations is increased, excellent and high yield herbicide-resistant two-line hybridization rice is cultured, the cultured herbicide-resistant two-line hybridization rice, routine rice varieties and the recurrent parent are subjected to matching, the hybridization later generations all possesses herbicide resistance, and weed control difficult problems in rice fields are solved effectively.

The invention provides a discharge outlet purification system and a purification method thereof. The purification system is carried at a discharge outlet of sewage, the sewage is discharged after being purified by the discharge outlet purification system, and the discharge outlet purification system comprises a dosing section, a pretreatment section, a filler treatment section, a biochemical treatment section and a filter membrane treatment section. According to the system, the space of the receiving water area of the discharge outlet is transformed, purification treatment of discharged waterof the discharge port is achieved, and the system has the advantages of being high in applicability, rapid, efficient, multifunctional, flexible in operation and the like.

The invention relates to construction of a rough Brucella strain of a recombinant chlamydia psittaci outer membrane protein MOMP gene and a vaccine thereof. A rough bovine Brucella attenuated strain RA343 is taken as a parent strain, a sucrose suicide plasmid is taken as a vector, the Chlamydia psittaci outer membrane protein MOMP gene containing a specific promoter sequence is inserted into a Brucella genome without trace after codon optimization, and the recombinant Brucella strain RA343-MOMP capable of efficiently expressing the Chlamydia psittaci outer membrane protein gene is successfullyconstructed. The recombinant strain not only retains the rough type characteristic of an original parent strain RA343 and has good immunoprotection on brucellosis (brucellosis). In addition, an MOMPantibody against chlamydia psittaci can be generated after the recombinant strain is used for immunizing animals, and accordingly immunoprotection on chlamydia psittaci is achieved. The vaccine prepared by the recombinant vaccine strain can simultaneously realize double immune protection on brucellosis and chlamydia psittaci after being used for immunizing animals.