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Preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus

A technology of Monochamus alternatus and whole protein, which is applied in the field of preparation of extracellular whole protein extract, and can solve problems such as ineffective effects

Inactive Publication Date: 2012-10-17
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Jiangsu used 3% fenitrothion to spray for several years, but the effect was not obvious

Method used

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  • Preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus
  • Preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus

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Experimental program
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Effect test

Embodiment Construction

[0018] Now in conjunction with the drug efficacy test of the present invention, further illustrate the beneficial effects of the present invention.

[0019] The preparation method of the extracellular whole protein extract of the control Monochamus alternatus comprises the following steps:

[0020] 1) Inoculate the Beauveria bassiana strain on PPDA medium, culture it in an incubator at 23°C for 16 days, scrape off the conidia, and dilute it with sterile water to 1.0×10 8 pcs mL - 1 The spore suspension was inoculated on the fermentation medium according to the spore suspension: fermentation medium volume ratio of 1:100, and then it was placed in a constant temperature shaking incubator at 25°C and 130rpm to cultivate for 7d, for subsequent use;

[0021] 2) Take the fermentation broth from step 1, centrifuge at 4°C and 4000rpm for 13 minutes, collect the supernatant, and vacuum filter it with 3 layers of filter paper to remove the mycelium suspended in the fermentation broth,...

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Abstract

The invention provides a preparation method for extracellular holoprotein extract used for controlling Monochamus alternatus, which belongs to the technical field of biological control for forest pests. The preparation method is characterized by comprising the following concrete steps: 1) carrying out inoculated culture on Beauveria bassiana strains so as to obtain fermentation broth; 2) separating the fermentation broth, collecting supernatant and removing mycelia so as to obtain a filtrate which is a crude extracellular holoprotein extract; 3) adding ethylene diamine tetraacetic acid into the crude extracellular holoprotein extract, adding ammonium sulfate powder under an ice bath condition, carrying out stirring and dissolving, standing an obtained mixture overnight and dissolving an obtained precipitate with a phosphate buffer solution so as to obtain a concentrate of the crude extracellular holoprotein extract; and 4) carrying out dialysis on the crude extracellular holoprotein extract, separating the concentrate and carrying out filtering and sterilization on obtained supernatant so as to obtain the extracellular holoprotein extract. The extracellular holoprotein extract prepared by using the preparation method provided in the invention has the advantages of strong insecticidal activity, capacity of controlling Monochamus alternatus, safety and high efficiency in usage, no pollution and a good control effect and is a good biological control reagent.

Description

technical field [0001] The invention belongs to the technical field of biological control of forestry pests, in particular to a preparation method of an extracellular whole protein extract for controlling Monochamus alternatus. Background technique [0002] Monochamus monochamus and the pine xylophilus disease it spreads have caused very serious damage to pine forests in my country. The control of monochamus monochamus and continuous control of pine xylophilus is a major problem that forestry workers need to solve urgently. At present, there are three main methods of controlling Monochamus alternatus: chemical control, using attractants to control long-cut monochamus and biological control. Chemical control has a relatively large negative impact. Biological control is an environmentally friendly plant protection technology. It is particularly important to find ecological control factors such as pathogenic microorganisms that can infect and reduce the number of pests. [00...

Claims

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Application Information

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IPC IPC(8): C07K1/30A01N63/04A01P7/04A01P17/00
Inventor 林海萍毛胜凤刘洪波
Owner ZHEJIANG FORESTRY UNIVERSITY
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