Novel bacteria strain and culture method and application thereof

A culture method and strain technology, applied in the field of new bacterial strains and their culture, can solve problems such as root-knot nematode disease

Active Publication Date: 2015-12-02
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, the present invention obtains a bacterium through systematic screening, and utilizes the bacterium or the metabolite of the bacterium to treat tomato seeds, which can induce tomato to produce resistance to root-knot nematode incognita during seed germination and growth, Thereby solving the problem of root-knot nematode disease

Method used

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  • Novel bacteria strain and culture method and application thereof
  • Novel bacteria strain and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cultivation of Pseudomonasputida Sneb821

[0019] Inoculate the bacterial strain of Pseudomonasputida (Pseudomonasputida) Sneb821 on the test tube culture medium, the medium formula is beef extract peptone medium, namely ingredients: beef extract 3g, yeast extract 1g, peptone 10g, sucrose 10g, agar 17 -20g, add distilled water to 1000mL. Incubate at 28°C for 3 days to obtain test tube species.

[0020] Then inoculate the test tube species into a 250mL triangular flask (150mL per bottle) liquid medium, and the medium formula is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3% -0.4%, 1%-2% sucrose, the rest is distilled water, pH is 6.8-7.0, fermentation temperature is 25-28°C, shaker speed is 150r min -1 , Fermentation time 2-3d. The resulting fermentation broth or finished product of the fermentation broth can be used for direct or indirect treatment of tomato seeds. Treated tomato seeds can reduce damage caused by root-knot nemat...

Embodiment 2

[0022] The fermentation of Pseudomonasputida Sneb821 was basically the same as that in Example 1, except that the liquid-cultured strain was inoculated into a 1000 L fermenter for fermentation, cultured at 28° C., pH 7.0, and fermented for 3 days.

Embodiment 3

[0024] Field experiment on control of root-knot nematode incognita by fermented product of Pseudomonasputida Sneb821

[0025] Field effect tests were carried out in greenhouses in Liqianhu Town, Tieling City, Liaoning Province and Gaosheng Town, Panjin City, Liaoning Province. The prepared fermentation stock solution is treated with seed coating with a volume ratio of 1:40, dried under aseptic conditions, and the treated tomato seeds are sown normally, and the tomato plants can produce resistance to root-knot nematode incognita at the seedling stage. resistance, and the induced resistance effect is relatively stable, and a good control effect has been achieved.

[0026] Step 1. Preparation of test preparations

[0027] The fermentation broth of Pseudomonasputida Sneb821 was prepared according to the aforementioned liquid fermentation culture method, to be used for later use.

[0028] Step 2. Preparation of seeds for testing

[0029] Tomato variety L-402 was provided by Vege...

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Abstract

The invention discloses a novel bacteria strain and a culture method and application thereof. The bacteria strain is pseudomonas putida with the preservation number of CGMCC No. 10729; the culture medium of the novel bacteria strain is prepared from the following raw materials in percentage by mass: 0.1-0.2% of a yeast extract, 0.5-0.6% of peptone, 0.3-0.4% of a beef extract and 1-2% of sucrose, pseudomonas putida (Sneb 821) or metabolite thereof has an activity of inducing tomatoes, can be used for seed treatment of tomatoes, can not only promote the growth of tomato plants and roots, but also has a great inhibiting effect on formation of meloidogyne incognita, and further solves the problem of prevention and treatment of root-knot nematodes.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a novel bacterial strain and its cultivation method and application. Background technique [0002] Root-knot nematode (Meloidogynespp.) is a highly specialized omnivorous phytopathogenic nematode with a wide range of hosts, often damaging more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, lettuce, and cabbage. It also aggravated soil-borne fungal diseases and some bacterial diseases. Root-knot nematode in southern my country is the most widely distributed and the most harmful. In recent years, with the adjustment of agricultural planting structure and global warming, the cultivated area of ​​protected crops has developed rapidly, and at the same time, it has provided a suitable environment for the occurrence and development of root-knot nematodes in the soil, making root-knot nematodes in the soil free Accumulate and proliferate, and the host ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/40
Inventor 陈立杰段玉玺赵丹王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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