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Duck reovirus, preparation method for antigen-antibody complex thereof and application of duck reovirus

A reovirus, antigen-antibody technology, applied in the directions of virus antigen components, biochemical equipment and methods, viruses/phages, etc., can solve the problems of duck reovirus prevention and treatment without obvious effect, and achieve significant economic benefits and social benefits

Active Publication Date: 2020-12-15
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method and application of duck reovirus and its antigen-antibody complex, to solve the technical problem that the traditional Chinese veterinary medicine has no obvious effect on the prevention and treatment of duck reovirus in the prior art

Method used

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  • Duck reovirus, preparation method for antigen-antibody complex thereof and application of duck reovirus
  • Duck reovirus, preparation method for antigen-antibody complex thereof and application of duck reovirus
  • Duck reovirus, preparation method for antigen-antibody complex thereof and application of duck reovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 prevents duck reovirus effect under laboratory conditions

[0050] 1.1 Test site and contact person

[0051] See Table 1 for details.

[0052] Table 1 Information on test sites for reovirus antigen-antibody complexes under laboratory conditions

[0053]

[0054] 1.2 Test plan

[0055] Source of healthy ducklings: Anhui Qiangying Duck Industry Group Co., Ltd. provides breeding eggs from a parent duck breeding farm in Cherry Valley. Chongqing Sanjie Zhongxin Biological Engineering Co., Ltd. hatches the chicks according to the duck hatching program, and selects healthy ducklings for the experiment . The characteristics of healthy ducklings: hatching on time, soft belly, well-absorbed navel, clean anus, thick, dry and shiny fluff, loud cry, lively action, quick response, newborn weight meets the requirements of this breed, and no physiological defects.

[0056] 60 1-day-old healthy Cherry Valley ducklings were randomly divided into 3 groups, 20 per group...

Embodiment 2

[0067] Embodiment 2 prevents duck reovirus effect under clinical conditions

[0068] 2.1 Clinical trial sites and contacts

[0069] See Table 3 for details.

[0070] Table 3 Information on clinical application sites of reovirus antigen-antibody complexes

[0071]

[0072] 2.2 Test plan

[0073] In 5 customer farms of Longchang County Livestock Kang Veterinary Medicine Trading Co., Ltd., the duck reovirus antigen-antibody complex was used for clinical prevention of duck reovirus. The above 5 farms were all in the process of raising ducks in the last batch. "Hepatosplenic necrosis" in ducks caused by reovirus infection in China. Three days before entering the ducklings, the technicians of Chongqing Sanjie Zhongxin Bioengineering Co., Ltd. will guide the disinfection of the brooding house and the adult duck house, and then purchase the ducklings to start breeding and testing.

[0074] The duck species used in this experiment is Lace Duck, which is the most widely bred meat...

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Abstract

The invention provides a duck reovirus SL strain; the strain is collected in China Center for Type Culture Collection in Wuhan University; the collection number is CCTCC No:V201970, and the inventionaims to solve the technical problem that traditional Chinese veterinary drugs in the prior art have no obvious effect on prevention and treatment of the duck reovirus. The use of an antigen-antibody complex of the duck reovirus can solve the difficult problem of clinical prevention and control of the duck reovirus, promotes income increasing for breeders, escorts healthy development of the duck breeding industry in China, and has remarkable economic and social benefits.

Description

technical field [0001] The invention relates to a preparation method and application of duck reovirus and an antigen-antibody complex thereof. Background technique [0002] Since 2014, a clinical symptom that has been prevalent in my country's duck breeding industry is mental depression, neck and wings drooping, weak feet, squatting, diarrhea and white or green loose stools; the pathological changes of dead ducks are mainly liver and spleen. The disease with necrotic foci is clinically called "liver and spleen necrosis" in ducks, and the pathogen is confirmed to be duck reovirus. [0003] Natural clinical disease occurs in ducks, geese, muscovy and muscovy ducks [1] , the susceptibility of shelduck, goose, muscovy duck and semi-muscle duck is equivalent, but the susceptibility of meat-type ducks such as Cherry Valley duck and Peking duck is higher. For example, once Cherry Valley duck and Peking duck are infected with duck reovirus, The morbidity rate of ducks less than 1 w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C07K16/10A61K39/15A61K39/42A61P31/14
CPCC12N7/00C07K16/10A61K39/12A61K39/42A61P31/14C12N2720/12221C12N2720/12234A61K2039/552A61K2039/55588A61K2039/54Y02A50/30
Inventor 张立武魏思远赵光伟杨晓伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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