The invention discloses antialcoholic sour milk, which contains vine root powder, taurine and soy peptide, which are fermented together. The sour milk is prepared by the following steps: examining raw pure milk, purifying the milk, mixing, homogenizing, sterilizing, cooling, inoculating, stirring, filling, fermenting, cooling, detecting and obtaining a finished product. The mixing process comprises: dispersing the vine root powder, vitamin C, taurine and soy peptide in pure milk in an amount which is 8 to 10 times the total amount of the vine root powder, vitamin C, taurine and soy peptide, and uniformly mixing the materials by stirring; filtering the mixture with a 120-mesh nylon sieve to remove impurities; and adding the mixture as well a stabilizer, white sugar and whey protein into pure milk at the same time for mixing. The method has the advantages that: food alcoholic relief instead of medicament relief is adopted; and the sour milk has high nutritive value and can protect gastric mucosa from damages of alcohol, improve the detoxifying function of the liver, reduce the interference of the alcohol with human metabolism and effectively prevent and treat headache, sickness, hangover and other symptoms caused by drunkenness.