83results about How to "Increase the total" patented technology

The invention discloses a method for producing a compound microbial preparation serving as fodder, which comprises the steps of microbial inoculum production, material proportioning, bacillus subtilis medium preparation, beer yeast medium preparation and lactobacillus plantarum medium preparation. According to the method, bacillus subtilis, beer yeast and lactobacillus plantarum are bred to form compound strains, so that the totality of the compound thalli is increased, and the advantages such as high strain survival rate, strong stability and the like are achieved; the compound thalli are mixed with ball-milling chaff, bran and oligoisomaltose in a proper proportion, and the optimum compounding proportion is formed for the preparation; good conditions are created for the formation of the internal environment of intestinal canals of livestock and poultry, the aerobic bacillus subtilis interacts with yeast to generate more nutrient substances, amylase, protease cellulose and the like; and meanwhile, oxygen in the intestinal canals of livestock and poultry can be consumed, excellent growth environment is created for propagation of lactic acid bacteria, intestinal canal functions of livestock and poultry are improved, the fodder conversion rate is improved, and the microecological balance of the intestinal canals of livestock and poultry is improved.

A green underground three-dimensional automatic parking facility. Multi-storey parking spaces are arranged around an underground lift shaft. The shunt carriages are driven by a lifting mechanism and can go up and down in the underground lift shaft. There is an entry and exit parking space next to the top of the lower lift shaft. platform and an intelligent management center for controlling and managing the work of the entire parking lot; a fixed pulley is installed on the top of the elevator shaft, and the two ends of a balance rope or chain that go around the fixed pulley are respectively connected with a counterweight and the shunt Compartment connection. The advantages of the present invention are: the radial cross track is used to guide the multiple parking spaces arranged along a circle on each floor, which greatly increases the number of parking spaces on each floor, thereby increasing the total number of parking spaces provided by an elevator shaft , increase the parking space by five to ten times, increase the volume ratio, save the ground, and reduce the cost; the counterweight weight of the lifting car can be adjusted according to the weight of the vehicle to achieve the best counterweight balance effect, ensuring the lift of the car. Stability and security.

The invention belongs to the technical field of stereo garage, in particular to a multiple exit and entrance cluster tower type stereo garage for a large-sized parking lot which needs to build a plurality of stereo garages to satisfy the parking demand. The multiple exit and entrance cluster tower type stereo garage comprises a plurality of tower type stereo garages, wherein the middle part of each stereo garage is provided with an elevator, a plurality of parking spaces are radially arranged with the elevator as the center, and the ground floor of the stereo garage is a parking passage. The garages are all radially arranged with the elevators as the centers, and gaps exist among the garages. The improvement comprises that each tower type stereo garage is alternately arranged when the garages are built, namely the parking space of one of the garages is inserted into the gaps among the parking spaces of other tower type garages, and the parking spaces are alternately arranged to ensure that the parking spaces are alternated and merged with each other to form the integral cluster tower type stereo garage. The multiple exit and entrance cluster tower type stereo garage ensures that the volume rate and the service efficiency of the prior tower type stereo garage are greatly improved again, provides more parking passages for temporarily parking vehicles at the same time, and improves the buffer capacity during peak hours of vehicle access.

The invention relates to a preparation method of fluorine-containing low-silicon sintering ore, belonging to the technical field of metallurgy. Raw materials are fluorine-containing iron ore concentrate, import fine ore, solvent and fuel, wherein the fluorine-containing iron ore concentrate accounts for 80-85% of sintered iron material, and the import fine ore accounts for 15-20% of the sintered iron material; F content in the iron ore concentrate is 0.1-0.3%, and grain composition of minus 200 meshes of the iron ore concentrate accounts for more than 85%; ratio of home grown iron ore concentrate A to home grown ultrafine iron ore concentrate is regulated, SiO2 content is controlled to be 4.0-4.2%, two solvents such as limestone flour and ground quick lime are used for controlling basicity to be 2.3-2.5, dolomite dust with coarse granularity or light roasting dolomite dust with fine granularity is used for controlling MgO content to be 2.1-2.3%. After low silicon sintering is carried out, the SiO2 content of the sintering ore is reduced, thus grade of the sintering ore is improved, metallurgy performance of the sintering ore is improved, reduction performance is improved to 86.94% from 85.14%, slag quantity is reduced, and the preparation method is beneficial to improving blast furnace index.

The invention discloses a low-temperature-resistant composting fermentation microbial inoculum with a deodorizing function and applications of the low-temperature-resistant composting fermentation microbial inoculum. The microbial flora contained in the low-temperature-resistant composting fermentation microbial inoculum provided by the invention can tolerate low temperature, and can normally grow at 15-20 DEG C, so that the fermentation air temperature of organic materials in the low-temperature environment is facilitated, in addition, the strains have the mutual promotion effects, therefore, a mutual synergistic effect is generated, and further, decomposing is accelerated. The experiment proves that during the composting process, after the low-temperature-resistant composting fermentation microbial inoculum is added into the organic materials for fermentation, the sum of effective microbes at the composting initial stage is improved, the temperature increase rate of the composted materials is accelerated, the anaerobic fermentation time is shortened, the decomposing process is accelerated, meanwhile, the discharge of sulfur-containing compounds and nitrogen-containing compounds is reduced, the physicochemical properties of the fermentation products are optimized, and in addition, the low-temperature-resistant composting fermentation microbial inoculum has a certain deodorizing function.

The invention provides a composition with multiple health-care functions and a preparation method thereof. The composition has the following raw materials by weight portion: 6 to 12 portions of pilose asiabell root, 6 to 12 portions of Chinese angelica, 2 to 15 portions of barbary wolfberry fruit, 2 to 15 portions of Chinese caterpillar fungus, 6 to 12 portions of radix astragali, 6 to 12 portions of hairy antler, 1 to 9 portions of American ginseng, 2 to 15 portions of tuber fleeceflower root, 5 to 18 portions of longan flesh, 5 to 18 portions of Chinese date flesh, 5 to 20 portions of walnut kernel, 5 to 18 portions of rock sugar, 1 to 15 portions of peach blossom, 1 to 15 portions of lily, 6 to 12 portions of donkey-hide glue, 750 to 8,000 portions of yellow wine, 1 to 9 portions of liquoric root and 2 to 15 portions of black bean. The composition adopts various famous and precious pure natural pollution-free raw materials, and is convenient to take, good in taste, easy in absorption, free from chemicals and artificially synthesized substances. The preparation method can release 18 kinds of amino acids in the composition such as lysine, glycin, arginine and 8 kinds of essential amino acids which cannot be synthesized by human bodies. The composition has high efficacy in nourishing liver and kidney and strengthening haematogenesis.

The invention relates to an animal feed additive, and in particular relates to a composite feed additive preparation technology based on gynostemma pentaphylla. According to the composite feed additive preparation technology, ACCC10634 bacillus subtilis and ACCC11016 Lactobacillus plantarun are respectively used as strains and are inoculated to an inorganic salt culture solution containing gynostemma pentaphylla micro powder to be cultured grade by grade; a tissue structure of the gynostemma pentaphylla is digested in a way that an intestinal environment of an animal body is simulated in vitro, so that effective components are released, and the extraction rate of the effective components of the gynostemma pentaphylla is improved; the efficacy of the gynostemma pentaphylla is enhanced; the composite feed additive preparation technology has a function of promoting the growth of pigs and livestock and can improve the disease resistance of the pigs and the livestock and increase the feed reward.

The invention discloses a full-suspension culture method for avian influenza virus. The full-suspension culture method comprises the following steps: step 1, taking EB66 cells for growth culture; step2, when the density of the EB66 cells grows to be suitable for virus inoculation, utilizing a fed-batch fluid-replacement virus inoculation technology to inoculate avian influenza virus into the EB66cells and performing virus multiplication culture; step 3, 24h after virus inoculation, sampling every 12h to determine HV titer of the virus and obtaining and storing the virus when the virus HA titer reaches to the maximum to obtain cultured avian influenza virus. According to the full-suspension culture method disclosed by the invention, the EB66 cells are utilized to perform avian influenza virus culture, so that the defect that a lot of miscellaneous protein is prone to being introduced into when a traditional chick embryo culture technology is utilized for production is overcome, and occurrence of chicken immunity side reaction is effectively reduced; meanwhile, titer and purity of the cultured avian influenza virus are improved; furthermore, production quality of the avian influenza vaccine is improved.

The invention discloses an efficient controlled release fertilizer favorably improving taste and freshness of ivy mosses. The fertilizer comprises the following components in parts by weight: oyster shells, polylactic acid, expanded graphite, thermally activated nano chernozem, pinecones, pomegranate barks, mosses, sugarcane ash, a soil beneficial microbial inoculant, bacitracin zinc, phytase, amino acid, ground calcium ammonium sulfate, boric acid, sodium selenite, chelated iron, potassium fulvate, lysine, a proper amount of hydrogen peroxide, a proper amount of ammonia water, a proper amount of water and the like. The coated controlled release fertilizer is prepared by using the pinecones, the pomegranate barks, the mosses and the sugarcane ash which are matched with nutritional substances through fermentation. The fertilizer is rich in nutrient elements such as fermented small molecular organic matters, calcium, iron, selenium and zinc, is abundant in nutrient and convenient for plants to absorb and utilize, so that the taste and freshness of the ivy mosses can be enhanced; by adopting the coating layer which can supply oxygen and dynamically adjust the porosity, the fertilizer efficiency time is long, organic matter nutrients can be released for a long time, efficient cultivation of the ivy mosses can be realized, and the fertilizer can be widely applied and popularized.

The invention provides a method for calibrating a relation between an actual road distance and a corresponding pixel distance in a traffic video. The method comprises the following steps of: dividing a road in a video image window into a plurality of sections, which are uneven in length and are connected end to end, along a road driving direction; establishing a secondary function relation between the actual road distance and the pixel distance in a corresponding video image along the driving direction in each section; calculating a secondary function coefficient of each section according to each road section end point measured in a road scene, the actual distance in one point and the corresponding pixel distance in the video image, so as to determine the secondary function relation between each section of the actual road distance and the pixel distance in the corresponding video image, and further obtain a sectioned secondary function relation between the actual road distance in the whole video window and the corresponding pixel distance. Compared with the prior art, the operation is greatly simplified, data including camera data, camera lens data, erections position and directions of a camera lens and the like do not need to be known, and the precision can be guaranteed. Compared with other linear function representations or other sectioned representation methods, the calibration precision is greatly improved.

The invention discloses an efficient slow-release fertilizer capable of promoting growth and development of strawberries. The fertilizer is prepared from the following components in parts by weight: oyster shell, graystone nanocellulose, carbon aerogel, activated nano yellow subsoil, silkworm excrement, bean cake dregs, peanut dregs, distiller's yeast, wine-related yeasts, urease inhibitors, ammonium bicarbonate, vegetable amino acids, compound sodium nitrophenolate, dicyandiamide, honey, brassinolide, a proper amount of hydrogen peroxide, a proper amount of ammonium hydroxide and the like. The silkworm excrement, the bean cake dregs, the peanut dregs and the distiller's yeast are adopted to match with a plant growth regulator, thus the coated slow-release fertilizer is prepared by fermenting, the fertilizer contains rich organic matters and is nutrient-rich, a coat film layer capable of supplying oxygen and dynamically regulating pores is adopted, the fertilizer effect time is long, nutrients can be released for a long time, cell viability of the strawberries can be improved, plant growth and development are accelerated, root and seedling growth is improved, the yield is increased, the ability of tolerating adversity is enhanced, efficient cultivation is realized, and the fertilizer can be widely applied and popularized.

The invention specifically discloses Penicillium griseofulvum CF3, which was preserved in the China Center for Type Culture Collection on April 15, 2016, with the preservation number being CCTCC NO: M 2016195. The invention also discloses application of the Penicillium griseofulvum in controlling and removing parasitic Orobanche weeds. The bacterial strain can remarkably inhibit seed germination of Orobanche aegyptiaca, improve microflora in soil, increase total number of bacteria in soil and the ratio of bacteria number to fungi amount and reduce total fungi amount of soil. Therefore, germination and growth of the parasitic Orobanche weed Orobanche aegyptiaca are inhibited, growth of crops is promoted, and yield of crops is raised.

The invention relates to a Chinese herbal medicine nutritional feed for cultivating giant salamanders. The Chinese herbal medicine nutritional feed is prepared from the following components in parts by weight: 9-17 parts of astragalus membranaceus, 3-6 parts of bighead atractylodes rhizome, 3-5 parts of divaricate saposhnikovia root, 5-9 parts of semen cuscutae, 4-6 parts of epimedium herb, 4-8 parts of prepared rehmannia root, 0.3-0.6 part of calcium hydrogen phosphate, 2-4 parts of algae powder, 1-2 parts of unsaturated fatty acid, 1-2 parts of bacillus subtilis, 0.3-0.6 part of L-lysine hydrochloride, 0.05-0.1 part of zinc citrate, 0.05-0.1 part of organic iron, 0.1 part of vitamin and 0.2 part of microelement. The vitamin comprises VA, VB1, VB2, VB6, VB12, VC, VD and VE. The microelement is microelement amino acid chelate or is one, two or three of zinc, iron and calcium. The Chinese herbal medicine nutritional feed for cultivating the giant salamanders can meet the nutritional requirements of the giant salamanders, the immunity and the sexual gland maturity in mating seasons of the giant salamanders can be improved, and the sperm viability of male giant salamanders is enhanced.

The invention discloses a preparation method of a natural preservative applied to iced fresh duck. The method comprises the following steps: 1) performing pretreatment: segmenting slaughtered Pekin-ducks (about 3.5kg) immediately, selecting duck, cleaning the surface of the duck with sterile pure water to wash off blood and dirt, then segmenting duck breast meat by using a sterile scissor, and then performing freezing storage; 2) preparing a natural preservative: separately preparing a rutin solution with a certain concentration from a rutin biological preservative purchased from the Chengdu Pufei De Biotech Company Limited, and conducting ultrasonic treatment to obtain the natural preservative; 3) performing soaking: soaking the iced fresh duck in the natural preservative, covering the duck with a preservative film, conducting standing and draining; and 4) performing packaging and refrigeration: subjecting the drained iced fresh duck to vacuum packaging by using fresh-keeping bags containing an antibacterial agent, and conducting refrigeration. The method has the advantages that the adopted food preservative is natural plant extract, so the quality of the fresh iced duck can be ensured under the condition, and the bacteriostatic effect is significant.

The invention relates to a nourishing oral liquid comprising donkey-hide gelatin as the main ingredient and bee honey as the modulating agent, as well as the following raw materials: acanthopanax root, schisandra fruit, prepared rehmannia root, haw and longan pulp. Its functions include nourishing blood, replenish vital essence, reinforcing the kidney and controlling nocturnal emission.

The invention discloses a method and device for distributing channel selection resources based on PUCCH format 1B. The method comprises the following steps: the network side device determines the number of the carrier aggregation users that can be distributed with 1B channel selection (1BCS) resources in the group according to a 1BCS resource reusing degree and a PUCCH resource pair number in the group; the network side device distributes the 1BCS resources in the group to the carrier aggregation users, and dispatches the carrier aggregation users according to the 1BCS resources in the group. According to the embodiment of the invention, the number of the carrier aggregation users accessed in a cell is increased through improving the 1BCS resource reusing degree on the premise that the uplink rate of the cell is not affected, so that the number of the carrier aggregation users accessed in the cell can be greatly increased; the problem that the newly accessed carrier aggregation users cannot perform carrier aggregation after the 1BCS resources in the cell are exhausted is solved.

The invention relates to new applications of PRDX2 and / or PRDX6 and concretely discloses new applications of PRDX2 protein and / or PRDX6 protein which are used for preparation of medicine compositions treating or preventing damage, aging or diseases caused by increase of reactive oxygen species (ROS). The invention also provides medicine compositions, food compositions or cosmetic compositions which employ the PRDX2 protein and / or the PRDX6 protein as active ingredients.

The invention relates to the technical field of cell culture containers for laboratories, and especially relates to a three-dimensional cell culture bottle capable of realizing continuous perfusion. The three-dimensional cell culture bottle comprises a culture bottle body, wherein the inner bottom of the culture bottle body is provided with continuous steps which are gradually lowered; a side wallis arranged on one side of the step surface of each step; a notch is formed in one end of the side wall; protrusions are evenly distributed on the step face, a liquid storage pool is arranged at oneend of the step face at the lowest position of the continuous steps, the step face where the liquid storage pool is located is connected with a supporting plate, the other end of the supporting plateextends to the position of a bottle neck, and an upper filling inlet and a lower flowing outlet are formed in the side wall of the culture bottle body. The three-dimensional cell culture bottle has the beneficial effects that a large-area cell three-dimensional adhesion culture space is created, and the single-bottle cell culture quantity is increased, so that the cell culture cost is indirectly reduced, the sustainable and stable perfusion of a cell culture solution can be realized, the continuous stability of a cell living environment is ensured, and the method is suitable for the growth andamplification of various adherent cells.

The invention relates to the technical field of cell isolated culture, particularly a digestive enzyme composition and application thereof, and an isolated culture method of umbilical epithelial cells. The digestive enzyme composition comprises hyaluronidase, collagenase VIII and pancreatin. The umbilical epithelial cell primary isolation adopts the hyaluronidase, collagenase VIII and pancreatin for combined digestion. The digestive enzyme composition has the advantages of mild properties, low cell trauma and high enzymolysis rate and greatly enhances the epithelial cell isolation efficiency.

The invention provides a method for rapidly obtaining autotetraploid wax gourds. The method comprises the following steps of step A, serving seedlings during interior leaf slight exposure in the period that two seed leaves of diploid wax gourds are completely unfolded as treatment materials, stripping immature leaves except a seedling growing point when two seed leaves of wax gourd seedlings are completely flattened to expose cores and dipping and impregnating the wax gourd seedling growing point through colchicine and step B, covering the seedlings after treatment through a sun-shade net. The wax gourd seedling treatment is performed by the method for rapidly obtaining the autotetraploid wax gourds and accordingly the induced mutation rate of the tetraploid wax gourds is 30 to 35%, the success rate of tetraploid induction is high, and the operation is simple and easy.

The invention provides a medicated diet composition for enhancing immunity. The medicated diet composition comprises, by weight, 60-75 parts of selenium-rich grifola frondosa, 35-45 parts of selenium-rich agaricus blazei murill, 30-40 parts of selenium-rich radix astragali, 2-10 parts of gypenoside and 2-10 parts of glutathione, wherein the selenium contents of the selenium-rich grifola frondosa,the selenium-rich agaricus blazei murill and the selenium-rich radix astragali are greater than 400 ppm. The invention further provides a preparation method of the medicated diet composition. The method is based on water extraction and repeated low-temperature extraction. The medicated diet composition has the advantages that components are reasonably compounded, the formulation is ingenious, andthe composition has the effect of regulating the balance of qi, blood, yin and yang of the human body and enhancing the body immunity. The preparation method has the advantages that through repeated low-temperature extraction, active ingredients can be extracted as many as possible on the premise of ensuring the activity of the active ingredients.

The invention provides a saline-alkaline land soil improvement method adopting a soil deep loosening agent containing halophile. The soil improvement method comprises a step of applying the soil deep loosening agent. The soil deep loosening agent comprises the components of, by mass, 10-16 parts of black mica flake, 2-5 parts of a halophilic methanogen culture, 4-8 parts of a salt marsh halobacterium culture, 12-15 parts of melaleuca stone, 7-15 parts of humic acid-polyacrylic acid, 6-10 parts of wood powdery activated carbon, 0.4-0.8 parts of an acrylamide water-retaining agent, 5-9 parts of boric acid, 3-7 parts of zinc sulfate, and 5-8 parts of bean meal. The method provided by invention has the following advantages: 5 months after soil improvement, soil salt content is 0.21-0.26%, soil pH is 7.2-7.4, and soil bulk density is 0.90-0.98g / cm<3>. Soil microorganism total amount, soil water-holding capacity and soil water stable aggregate content are improved, and soil nutrient is increased.

The invention provides a compound protein powder easy to absorb. The compound protein powder is prepared from soybean protein powder, yeast protein powder and gluten. A preparation method of the compound protein powder comprises the steps that the protein powder, the yeast protein powder and the gluten are weighed and mixed to obtain a raw material mixture, then, water is used for preparing the raw material mixture into a raw material solution with the mass-volume concentration being 10-15%, and uniform stirring is performed; papain or alkaline protease is added into the raw material solution for enzymolysis, after enzymolysis is ended, filtering is performed through a 200-mesh screen to obtain a filtrate, the filtrate is concentrated, and spray drying is performed after concentration. The protein powder is small in molecular weight, easy to absorb, low in cost and abundant in nutrition.

The invention discloses a high-quality high-yield fertilization method for continuous cropping of sweet potatoes. The method is used for fertilizing sweet potatoes planted in a continuous cropping mode, and belongs to the technical field of crop cultivation. The invention particularly relates to the high-quality high-yield fertilization method for continuous cropping of sweet potatoes to overcomethe problems of low yield and poor quality caused by existing continuous cropping planting of sweet potatoes. According to the invention, 150-200 kilograms of a balanced biological organic-inorganic compound fertilizer is applied to each mu of land in a bottom application manner; 30-50 kg of a high-concentration high-potassium humic acid complex organic-inorganic compound fertilizer is applied once to each mu of land through flushing in a potato block expansion period; high-content organic matter, humic acid and polyglutamic acid in the biological organic-inorganic compound fertilizer undergoa deep complexing reaction with nitrogen, phosphorus and potassium, so water retention and fertilizer retention are more efficient, bottom application effect is better, and fertilizer damage hardly occurs when an application amount is increased; in addition, the utilization rate of nitrogen, phosphorus and potassium can reach 70% or above, a base fertilizer is applied heavily, topdressing is conducted slightly, the yield of the continuously-cropped sweet potatoes can reach 4500-6500 kg per mu, the quality of the sweet potatoes is high, and continuous cropping harm and water, fertilizer and manual input are reduced.

The invention provides an embroidery pattern thumbnail display method of a household computerized embroidery machine. The method comprises the following steps: step A. generating pattern thumbnail data according to pattern file data; step B. generating a corresponding thumbnail according to the pattern thumbnail data; step C. setting a delay counter to perform delayed time processing on animation flicker of the thumbnail, and selecting reversely displayed color block thumbnails according to a current color block value when delay counting number of the delay counter is reached; and step D. refreshing and displaying the thumbnail. The method can help achieve visual expression effect and improve humanized design of products by adding a thumbnail generation method when a pattern data flow is processed without providing any hardware devices and additional processing tools.

The invention provides a natural three-primary color yoghourt and a preparation method thereof. The natural three-primary color yoghourt is prepared from raw milk, any one, two or three-primary color pigments of and additives, wherein the three-primary color pigment consists of a red natural pigment, a green natural pigment and a blue natural pigment; red is a monascus pigment, green is sodium copper chlorophyllin, and blue is gardenia blue pigment. According to the natural three-primary color yoghourt provided by the invention, three primary colors are used as yoghourt base materials, the natural pigments are added into the yoghourt, and yoghourt products of different color series can be obtained according to additive amount of the pigments and a ratio of the red pigment to the green pigment to the blue pigment; the color of the yoghourt is richened to attract consumers; nutritive value and a health-care function of the yoghourt can also be enhanced, and various demands of consumers in different ages are fully met. The natural pigments have an antioxidant function, and are capable of scavenging free radicals; by adding the natural pigments into the yoghourt, the nutritive value and the health-care function of the yoghourt can be enhanced, and the yoghourt can easily get favor of the consumers.

The invention provides a traditional Chinese medicine dry powder for improving the immunity of piglets, comprising the following raw materials in parts by weight: 5-20 parts of astragalus, 3-12 parts of Codonopsis pilosula, 2-12 parts of angelica, 1-7 parts of tangerine peel and 2-10 parts of poria cocos The present invention also includes a preparation method of traditional Chinese medicine dry powder for improving piglet immunity. The composition of the invention is simple, the production cost is low, the digestive system of the piglet can be improved, and its immunity can be improved. The invention has no toxic and side effects, does not produce drug resistance, does not have drug residues, and is healthy and environmentally friendly; the preparation method of the invention is simple , can be widely used in production.

The invention discloses a perfusion type bioartificial liver reactor based on a double-layered nitrocellulose membrane, which comprises a box body and a cell scaffold arranged in an intracavity of the box body, wherein a liquid inlet, a liquid outlet, an air inlet and an air outlet are arranged on the box body, and the cell scaffold is double-layered nitrocellulose membranes; the two long sides of the double-layered nitrocellulose membranes cling to the inner walls of the front side and the back side of the box body, and the two ends of the double-layered nitrocellulose membranes are fixed onthe inner walls of the left side and the right side of the box body; and the double-layered nitrocellulose membranes divide the intracavity of the box body into upper and lower independent spaces, and a culture medium flows in the upper and lower independent spaces to exchange substances. An interval space between the two layers of the double-layered nitrocellulose membrane is used as a three-dimensional growth space of liver cells, and the cells grow in the interval space, so that substances are fully transmitted and the damage of shearing force to the cells when the liquid flows can be simultaneously avoided, and simultaneously the immune isolation effect can be achieved.