Method for rapidly obtaining autotetraploid wax gourds

A technology of autotetraploid and wax gourd, which is applied in the field of obtaining tetraploid materials of wax gourd, can solve problems such as uncertain mutagenesis direction, and achieve the effect of simple and easy operation, high success rate, and reduced fruit-setting nodes

Inactive Publication Date: 2015-08-19
广西现代农业科技示范园
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Problems solved by technology

Physical mutagenesis refers to the treatment of the plant itself by physical methods such as X-rays, ?-rays, and ultraviolet rays to obtain polyploid plants, but the direction of mutagenesis is uncertain, and the ploidy of plants produced by mutagenesis is diverse; chemical mutagenesis is Refers to the method of obtaining polyploidy by using chemical reagents, such as colchicine, amisulfame, Fuminlong, naphthalene ethane, isoauxin, indole acetic acid, etc. to treat plants

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  • Method for rapidly obtaining autotetraploid wax gourds
  • Method for rapidly obtaining autotetraploid wax gourds
  • Method for rapidly obtaining autotetraploid wax gourds

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Embodiment Construction

[0017] Below in conjunction with accompanying drawing, preferred embodiment of the present invention is described in further detail:

[0018] The diploid wax gourd inbred lines of the 8th generation of homozygous selfing were soaked and germinated, and sowed after 70% of the seeds were white. When the 2 cotyledons of wax gourd seedlings are flat and slightly exposed, the young leaves outside the growth point of the seedlings are peeled off, and the colchicine mutagenesis treatment is carried out. The specific test is as follows:

[0019] ① When the 2 cotyledons of the wax gourd seedlings are completely flattened and slightly exposed, the young leaves outside the growth point of the seedlings are peeled off, and the growth points of the wax gourd seedlings are soaked with 0.2% colchicine at 7:00-9:00 every morning. 1~2 drops, continuous treatment for 7 days.

[0020] ②During colchicine treatment, it is best to keep the day and night temperature at 18~20°C during the day and 15...

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Abstract

The invention provides a method for rapidly obtaining autotetraploid wax gourds. The method comprises the following steps of step A, serving seedlings during interior leaf slight exposure in the period that two seed leaves of diploid wax gourds are completely unfolded as treatment materials, stripping immature leaves except a seedling growing point when two seed leaves of wax gourd seedlings are completely flattened to expose cores and dipping and impregnating the wax gourd seedling growing point through colchicine and step B, covering the seedlings after treatment through a sun-shade net. The wax gourd seedling treatment is performed by the method for rapidly obtaining the autotetraploid wax gourds and accordingly the induced mutation rate of the tetraploid wax gourds is 30 to 35%, the success rate of tetraploid induction is high, and the operation is simple and easy.

Description

technical field [0001] The invention relates to a method for obtaining tetraploid wax gourd materials, in particular to a method for rapidly obtaining autotetraploid wax gourd. Background technique [0002] At present, there are mainly the following methods for obtaining polyploidy: ①Natural occurrence, which means that in nature, through climate changes such as lightning, temperature and other climatic conditions and environmental stresses, somatic cells may divide during the growth of plants. Chromosome doubling occurs, resulting in polyploid plants. Natural occurrence requires a long time and special environmental conditions, and the frequency of polyploid formation is low. ②Artificial mutagenesis is mainly divided into physical mutagenesis and chemical mutagenesis. Physical mutagenesis refers to the treatment of the plant itself by physical methods such as X-rays, ?-rays, and ultraviolet rays to obtain polyploid plants, but the direction of mutagenesis is uncertain, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 万正林邓俭英武鹏李立志周艳霞刘朝安
Owner 广西现代农业科技示范园
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