A kind of breeding method of rose polyploid variety

A breeding method, polyploid technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of easily damaged plant materials, low survival rate of seedlings, low doubling power, etc., and the test conditions are easy to control , good cold resistance, and the effect of improving the success rate

Inactive Publication Date: 2018-01-30
源涌生物高科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the most convenient and effective ways to use in actual work is: root soaking method, soak the roots of the plant in chemical reagents, wash the roots after soaking, and then plant the plants in the soil. This method is mostly used to double the production of distant hybrids The infertile hybrids and the necessary microspore unit seedlings that have not been doubled by other methods, but the root soaking method requires a large amount of medicine, and the cost is high, and after transplanting, the survival rate of the seedlings is low; After the plant seeds are soaked in chemical reagents, they are transferred to the pre-made medium for cultivation. This method is very simple, but the doubling power is low; injection method, injecting chemical reagents into the stem tip, receptacle or flower bud , this method is efficient, labor-saving, and low-cost, but it is easy to damage the plant material during injection, and the success rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for cultivating rose polyploid varieties, comprising the following steps:

[0025] (1) When the female parent roses are budding, move the female parent roses into a room at -5~0°C for 1~2 hours of pretreatment, and use low temperature to kill the viruses of the female parent roses;

[0026] (2) After the pretreatment of step (1), the female parent rose is moved into a room at room temperature at a temperature of 18±3°C; the flower buds of the female parent rose are induced by spraying with colchicine;

[0027] The specific process of spray induction is as follows: select a plastic bag with two ends open, one end is open and tied on the end of the receptacle close to the flower branch, and the other end is opened and tied on an indwelling tube. When the indwelling tube is not in use, the free end is closed with a stopper. When opening the stopper, spray a colchicine solution with a concentration of 0.3% into the plastic bag in the form of a spray; then immediate...

Embodiment 2

[0031] A method for cultivating rose polyploid varieties, comprising the following steps:

[0032] (1) When the female parent roses are budding, move the female parent roses into a room at -5~0°C for 1~2 hours of pretreatment, and use low temperature to kill the viruses of the female parent roses;

[0033] (2) After the pretreatment of step (1), the female parent rose is moved into a room at room temperature; the flower buds of the female parent rose are induced by spraying with colchicine;

[0034] The specific process of spray induction is as follows: select a plastic bag with two ends open, one end is open and tied on the end of the receptacle close to the flower branch, and the other end is opened and tied on an indwelling tube. When the indwelling tube is not in use, the free end is closed with a stopper. When opening the stopper, spray 0.2% colchicine solution into the plastic bag in the form of spray; then immediately spray 0.8% dimethyl sulfoxide and 1% sorbitol mixed ...

Embodiment 3

[0038] A method for cultivating rose polyploid varieties, comprising the following steps:

[0039] (1) When the female parent roses are budding, move the female parent roses into a room at -5~0°C for 1~2 hours of pretreatment, and use low temperature to kill the viruses of the female parent roses;

[0040] (2) After the pretreatment of step (1), the female parent rose is moved into a room at room temperature; the flower buds of the female parent rose are induced by spraying with colchicine;

[0041] The specific process of spray induction is as follows: select a plastic bag with two ends open, one end is open and tied on the end of the receptacle close to the flower branch, and the other end is opened and tied on an indwelling tube. When the indwelling tube is not in use, the free end is closed with a stopper. When opening the stopper, spray 0.5% colchicine solution into the plastic bag in the form of spray; then immediately spray 0.5% dimethyl sulfoxide and 3% sorbitol mixed ...

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PUM

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Abstract

The invention discloses a method for cultivating a rose polyploidy variety. The method comprises a step (1) of moving female parent roses into a room at -5-0 DEG C when the female parent roses bear buds, performing pretreatment for 1-2 h, and using low temperature to kill viruses of the female parent roses; a step (2) of moving the female parent rose into a normal temperature room, and adopting colchicine to perform spraying method induction on buds of the female parent roses; a step (3) of collecting pollen of male parent roses to perform artificial pollination during a bud crack period, applying the pollen to stigmas of the female parent roses, performing daily management after the pollination, and obtaining rose seeds; a step (4) of performing flower field planting or soilless culture on the rose seeds, taking stem tips or root tips after plantation and adopting improved phenol fuchsine dyeing method to perform slide preparation, performing observation and camera shooting under a microscope, and checking the number of chromosomes of the plant, wherein the plant with a chromosome number of 21 is the obtained rose polyploidy variety. The method is high in mutagenesis success rate and low in cost, and test conditions are easy to control.

Description

technical field [0001] The invention relates to a method for cultivating flowers, in particular to a method for cultivating polyploid varieties of roses. Background technique [0002] Rose is a traditional famous flower in my country. It belongs to Rosaceae Rosa deciduous shrubs. It has bright colors and pleasant aroma. It is not only an excellent landscaping and soil and water conservation plant, but also a precious Chinese medicinal material, and an important raw material for the food industry and spice industry. Its flowers can be used to make spices, scented tea and food, especially the rose essential oil extracted from roses has high economic value and is known as "liquid gold" in the international market. Its fruits are rich in various trace elements, especially vitamin C content It is very tall and can be used to make jam and juice; its flower buds, fruits and roots can be used as medicine, which has the effects of regulating qi, promoting blood circulation and nouris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H1/02
CPCA01H1/02A01H1/08
Inventor 黄泳葛
Owner 源涌生物高科技有限公司
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