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The application discloses a kind of hydrophilic-hydrophobic heterogeneous wrinkle polymer microspheres and its preparation method and application.The polymer microspheres of the application are prepared by suspension-interface polymerization based on oil phase solution and water phase solution, wherein the preparation raw materials of oil phase solution include hydrophobic monomer, swelling solvent and initiator, and the mass-volume ratio is 3-5 mL:1-4 mL:0.05-0.2 g;The preparation raw materials of water phase solution include water-soluble monomer, stabilizer and solvent, and the mass-volume ratio is 0.5-2 mL:1-2 g:75-100 mL.The polymer microspheres prepared by the method of the application have good particle size uniformity, and the surface of microsphere presents wrinkle morphology, has larger specific surface area, and can be applied in the fields of drug carrier, catalytic carrier or biosensor etc.