A kind of preparation method of spinach traditional Chinese medicine microbial fertilizer
A microbial fertilizer, microorganism technology
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Embodiment 1
[0025] A preparation method of spinach traditional Chinese medicine microbial fertilizer, comprising the following steps:
[0026] (1) Fermentation and cultivation of microorganisms:
[0027] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slope of PDA medium, the initial pH of the medium is controlled at 6; the culture temperature is controlled at 20°C; after 20 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 30° C., and cultivates for 48 hours;
[0028] ②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium, the initial pH of the medium is controlled at about 7, the temperature is 20°C, culture for 16 days, and the temperature is raised to 30°C to continue the culture Af...
Embodiment 2
[0038] A preparation method of spinach traditional Chinese medicine microbial fertilizer, comprising the following steps:
[0039] (1) Fermentation and cultivation of microorganisms:
[0040] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slope of PDA medium, the initial pH of the medium is controlled at 7; the culture temperature is controlled at 30°C; after 16 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 28° C., and cultivates for 56 hours;
[0041]②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium, the initial pH of the medium is controlled at about 7, the temperature is 22°C, culture for 14 days, and the temperature is raised to 29°C to continue the culture Aft...
Embodiment 3
[0051] 1, a kind of preparation method of spinach traditional Chinese medicine microbial fertilizer is characterized in that, comprises the following steps:
[0052] (1) Fermentation and cultivation of microorganisms:
[0053] ① Inoculate Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus jelly-like on the slant of PDA medium, the initial pH of the medium is controlled at 8; the culture temperature is controlled at 40°C; after 12 hours of culture, the bacterial suspension is made , then inoculate the prepared bacterium suspension in the PDA liquid medium according to the inoculum size of 2% in proportion, and cultivate with shaking (shaking table rotation number is 160 rpm), and the temperature is controlled at 30° C., and cultivates for 48 hours;
[0054] ②Put Frankia, Ichthyospora, and Geodermophilus on the modified Camp-BAP medium. The initial pH of the medium is controlled at about 7, and the temperature is 25°C. Cultivate for 12 days, and then raise the tempe...
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