Three-dimensional cell culture bottle capable of realizing continuous perfusion

Abstract

The invention relates to the technical field of cell culture containers for laboratories, and especially relates to a three-dimensional cell culture bottle capable of realizing continuous perfusion. The three-dimensional cell culture bottle comprises a culture bottle body, wherein the inner bottom of the culture bottle body is provided with continuous steps which are gradually lowered; a side wallis arranged on one side of the step surface of each step; a notch is formed in one end of the side wall; protrusions are evenly distributed on the step face, a liquid storage pool is arranged at oneend of the step face at the lowest position of the continuous steps, the step face where the liquid storage pool is located is connected with a supporting plate, the other end of the supporting plateextends to the position of a bottle neck, and an upper filling inlet and a lower flowing outlet are formed in the side wall of the culture bottle body. The three-dimensional cell culture bottle has the beneficial effects that a large-area cell three-dimensional adhesion culture space is created, and the single-bottle cell culture quantity is increased, so that the cell culture cost is indirectly reduced, the sustainable and stable perfusion of a cell culture solution can be realized, the continuous stability of a cell living environment is ensured, and the method is suitable for the growth andamplification of various adherent cells.

Description

technical field [0001] The invention relates to the technical field of laboratory cell culture containers, in particular to a sustainable perfusion three-dimensional cell culture bottle. Background technique [0002] Cell culture can be used in many fields such as basic research, drug discovery and cell therapy. However, the culture environment created by most cell culture flasks is a two-dimensional culture environment, which cannot accurately simulate the three-dimensional environment of cells in the body. Tissue matrix, material preparation and material disinfection are laborious and expensive, and cells are not easy to be eluted and harvested, and forced elution will cause a large number of cell debris. Moreover, when cells are cultured in cell culture flasks, the medium needs to be changed every 1-3 days, and the concentration of cell metabolites and glucose fluctuates, which seriously affects the state of the cells. Therefore, it is necessary to find a more efficient...

AI Technical Summary

Problems solved by technology

However, the culture environment created by most cell culture flasks is a two-dimensional culture environment, which cannot accurately simulate the three-dimensional environment of cells in the body. Tissue matrix, material preparation and material disinfection are laborious and expensive, and cells are not easy to be eluted and harvested, and forced elution will cause a large number of cell debris

Moreover, cells are cultured in cell culture flasks, and the medium needs to be changed every 1-3 days. The concentration of cell metabolites and glucose fluctuates, which seriously affects the state of cells.

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