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Penicillium griseofulvum and application thereof

A technology for the control of Penicillium griseus, applied in the application, chemicals for biological control, biocides, etc., to achieve the effect of reducing the total number, increasing the ratio, and inhibiting germination

Inactive Publication Date: 2017-06-30
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary test results show that the antagonistic fungus Penicillium griseofulvin has the function of inhibiting the pathogenic fungi of various plant soil-borne diseases, and has a good control effect on soil-borne root diseases, but there is no report that Penicillium griseoflavin has the function of preventing and eradicating

Method used

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  • Penicillium griseofulvum and application thereof
  • Penicillium griseofulvum and application thereof
  • Penicillium griseofulvum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Inhibition of the Germination of Gualiedang Seeds by Fermentation Broth of Penicillium grisea

[0036] 1. Test method:

[0037] (1) Preparation of Penicillium grisea chrysogenum fermentation broth

[0038] The fermented liquid of Penicillium grisea is carried out by means of known liquid shaker culture, and the stoste (every milliliter of fermented liquid contains 3.5 mg dry mycelia) is diluted 10, 10 respectively 2 、10 3 、10 4 Doubling later for later use. The Gaoshi No. 1 liquid medium and PDA liquid medium not inoculated with Penicillium griseofulvin were used as controls respectively, and repeated 3 times.

[0039] (2) Seed germination test

[0040] Firstly, glass fiber filter paper with a diameter of 8mm was placed in a new petri dish, then 20 μL of fermentation broth, 1 piece of pre-cultivated melon seeds and 20 μL of GR24 (0.1 mg L -1 ) sequentially added to the glass fiber filter paper. Add sterile water and GR24 (0.1mg L -1 ) instead of the treatment of...

Embodiment 2

[0051] Inhibition of Methanol Extract of Penicillium grisea on Seed Germination of Gualiedang

[0052] 1. Test method

[0053] (1) Preparation of Penicillium grisea methanolic extract

[0054] Weigh 0.050g of Penicillium grisea and place it in a 1.5mL centrifuge tube, then add 1mL of methanol, ultrasonicate for 30min and then 6000r min -1 Centrifuge for 2min. The supernatant was regarded as the stock solution, diluted 10 and 100 times for use, and repeated 3 times.

[0055] (2) Seed germination test

[0056] Carry out according to above-mentioned test method that fermented liquid suppresses the seed germination of Gua Liedang, replace fermented liquid with thalline methanol extraction liquid, and test repeats 3 times.

[0057] 2. Test results

[0058] See Table 2 for the results of the methanol extract of Penicillium grisea chrysogenum inhibiting the germination of Gualedang seeds. The 50.0mg / mL thalline methanol extract of Penicillium grisea can inhibit the germination ...

Embodiment 3

[0063] pot experiment

[0064] 1. Test method:

[0065] The pot experiment was conducted from May to September 2014 at the Guyuan Ecological Experimental Station of the Institute of Soil and Water Conservation, Northwest Agriculture and Forestry University, Ningxia Hui Autonomous Region, China.

[0066] According to the results of indoor experiments, Penicillium grisea was used in pot experiments and was inoculated into the soil in the form of fungal agent. The Penicillium grisea fungus was prepared according to the method of Xue et al.'s 2013 foreign language literature "Isolation and evaluation of rhizosphere actinomycetes with potential application for biocontrol of Verticillium wiltof cotton", and the number of viable spores in the fungus was 10 11 g -1 . The soil used for potting is black loam. First, mix the soil and fine sand in a volume ratio of 1:1, and then add urea (20mg kg -1 ), calcium superphosphate (50mg kg -1 ), Guariedang seeds (3.4mg kg -1 ) and Penicil...

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Abstract

The invention specifically discloses Penicillium griseofulvum CF3, which was preserved in the China Center for Type Culture Collection on April 15, 2016, with the preservation number being CCTCC NO: M 2016195. The invention also discloses application of the Penicillium griseofulvum in controlling and removing parasitic Orobanche weeds. The bacterial strain can remarkably inhibit seed germination of Orobanche aegyptiaca, improve microflora in soil, increase total number of bacteria in soil and the ratio of bacteria number to fungi amount and reduce total fungi amount of soil. Therefore, germination and growth of the parasitic Orobanche weed Orobanche aegyptiaca are inhibited, growth of crops is promoted, and yield of crops is raised.

Description

technical field [0001] The invention relates to the technical field of parasitic weed control, in particular to a penicillium grisea and application thereof. Background technique [0002] The genus Orobanche spp. includes more than 200 species, many of which are root parasitic weeds of vegetables and crops. Due to the lack of leaves and chloroplasts, Redang completely depends on absorbing water and nutrients from the host, thus causing serious damage to the host plant. Melon (Phelipanche aegyptiaca Pers.) belongs to the genus Phelipanche, widely distributed in southern Europe, the Mediterranean region, northern Africa, southern Asia and China, and can be parasitic in Solanaceae, Fabaceae, Umbelliferae and Compositae, etc. crops. [0003] As the damage caused by radang to agricultural production is becoming more and more serious, and radang continues to spread and infect new areas, it is urgent to find measures that can effectively control radang. Currently commonly used c...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P13/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 陈杰马永清薛泉宏
Owner NORTHWEST A & F UNIV
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