Penicillium griseofulvum and application thereof

A technology for the control of Penicillium griseus, applied in the application, chemicals for biological control, biocides, etc., to achieve the effect of reducing the total number, increasing the ratio, and inhibiting germination

Inactive Publication Date: 2017-06-30
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary test results show that the antagonistic fungus Penicillium griseofulvin has the function of inhibiting the pathogenic fungi of various plant soil-borne di

Method used

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  • Penicillium griseofulvum and application thereof
  • Penicillium griseofulvum and application thereof
  • Penicillium griseofulvum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] Inhibition of fermentation broth of Penicillium griseofulum on the germination of melon seeds

[0036] 1. Test method:

[0037] (1) Preparation of fermentation broth of Penicillium griseofulvum

[0038] The fermentation broth of Penicillium griseophorum was cultured in a well-known liquid shaker, and the original solution (3.5mg dry hypha per ml of fermentation broth) was diluted by 10 and 10 respectively. 2 , 10 3 , 10 4 After doubling. The Gauss No. 1 liquid medium and PDA liquid medium without inoculation of Penicillium griseoideum were used as controls, and repeated 3 times.

[0039] (2) Seed germination test

[0040] First put a glass fiber filter paper sheet with a diameter of 8mm in a new petri dish, and then put 20μL of fermentation broth, 1 piece of pre-cultured melon seeds and 20μL GR24 (0.1mg L -1 ) Are sequentially added to the glass fiber filter paper. Add sterile water and GR24 (0.1mg L -1 ) Instead of the fermentation broth, it is used to detect...

Example Embodiment

[0050] Example 2

[0051] Inhibition of the methanol extract of Penicillium griseofulvum on the germination of melon seeds

[0052] 1. Test method

[0053] (1) Preparation of methanol extract of Penicillium griseofulvum mycelium

[0054] Weigh 0.050g of Penicillium griseofulvum mycelium in a 1.5mL centrifuge tube, then add 1mL of methanol, sonicate for 30min and then 6000r min -1 Centrifuge for 2 min. The supernatant was regarded as the original solution, diluted 10 and 100 times and then used as a reserve, repeated 3 times.

[0055] (2) Seed germination test

[0056] Carry out the test method for inhibiting the germination of melon seeds by the above fermentation broth, replace the fermentation broth with the methanol extract of the bacteria, and repeat the test 3 times.

[0057] 2. Test results

[0058] See Table 2 for the test results of the methanol extract of Penicillium griseofulvum in inhibiting the germination of melon seeds. The methanol extract of 50.0mg / mL of Penicillium grise...

Example Embodiment

[0062] Example 3

[0063] Pot experiment

[0064] 1. Test method:

[0065] The pot experiment was carried out at the Guyuan Ecological Experiment Station of the Institute of Soil and Water Conservation, Northwest A&F University, Ningxia Hui Autonomous Region, China from May to September 2014.

[0066] According to the results of indoor tests, Penicillium griseofulvum was used in pot experiments and inoculated into the soil in the form of inoculants. The penicillium griseopyrum inoculum was prepared in accordance with the 2013 foreign literature "Isolation and evaluation of rhizosphereactinomycetes with potential application for biocontrol of Verticillium wiltof cotton", and the number of viable spores in the inoculum was 10 11 g -1 . The soil used for potting is black loess soil. First, mix the soil and fine sand at a volume ratio of 1:1, and then add urea (20mg kg -1 ), superphosphate (50mg kg -1 ), melon seeds (3.4mg kg -1 ) And Penicillium griseoate (1g kg -1 ) And mix thoroughly....

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Abstract

The invention specifically discloses Penicillium griseofulvum CF3, which was preserved in the China Center for Type Culture Collection on April 15, 2016, with the preservation number being CCTCC NO: M 2016195. The invention also discloses application of the Penicillium griseofulvum in controlling and removing parasitic Orobanche weeds. The bacterial strain can remarkably inhibit seed germination of Orobanche aegyptiaca, improve microflora in soil, increase total number of bacteria in soil and the ratio of bacteria number to fungi amount and reduce total fungi amount of soil. Therefore, germination and growth of the parasitic Orobanche weed Orobanche aegyptiaca are inhibited, growth of crops is promoted, and yield of crops is raised.

Description

technical field [0001] The invention relates to the technical field of parasitic weed control, in particular to a penicillium grisea and application thereof. Background technique [0002] The genus Orobanche spp. includes more than 200 species, many of which are root parasitic weeds of vegetables and crops. Due to the lack of leaves and chloroplasts, Redang completely depends on absorbing water and nutrients from the host, thus causing serious damage to the host plant. Melon (Phelipanche aegyptiaca Pers.) belongs to the genus Phelipanche, widely distributed in southern Europe, the Mediterranean region, northern Africa, southern Asia and China, and can be parasitic in Solanaceae, Fabaceae, Umbelliferae and Compositae, etc. crops. [0003] As the damage caused by radang to agricultural production is becoming more and more serious, and radang continues to spread and infect new areas, it is urgent to find measures that can effectively control radang. Currently commonly used c...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P13/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 陈杰马永清薛泉宏
Owner NORTHWEST A & F UNIV
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