Low-temperature-resistant composting fermentation microbial inoculum with deodorizing function and applications of low-temperature-resistant composting fermentation microbial inoculum

A bacterial agent and composting technology, which is applied in the field of compound microbial bacterial agents, can solve the problems of reducing the fertilizer efficiency of compost products, the number of effective microorganisms is small, and the compost fermentation temperature is difficult

Pending Publication Date: 2016-12-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the method of aerobic composting, these organic wastes can be turned into high-quality organic fertilizers. The traditional composting method requires a certain amount of time to reproduce due to the small number of effective microorganisms in the initial stage. The fermentation time is long, the odor is large, and the loss of nutrients is large , serious waste of resources and other issues, especially in autumn and winter in the north, due to the low ambient temperature outside, the problem of difficulty in starting the temperature of compost fermentation is particularly prominent
[0003] In addition, the composting process is often accompanied by the generation of foul-smelling gases, causing loss of appetite, dizziness, nausea, and vomiting, which has become an important restrictive factor affecting the development of the composting industry
The main components of malodorous gases include sulfides, ammonia, lower aliphatic amines, aromatic hydrocarbons, hydroxyl compounds, alcohols, lower fatty acids and indole, etc. Among them, ammonia and hydrogen sulfide are the main components of malodorous gases. The volatilization of these gases will pollute It damages the environment and loses nitrogen, which reduces the fertilizer efficiency of compost products

Method used

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  • Low-temperature-resistant composting fermentation microbial inoculum with deodorizing function and applications of low-temperature-resistant composting fermentation microbial inoculum
  • Low-temperature-resistant composting fermentation microbial inoculum with deodorizing function and applications of low-temperature-resistant composting fermentation microbial inoculum
  • Low-temperature-resistant composting fermentation microbial inoculum with deodorizing function and applications of low-temperature-resistant composting fermentation microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] Embodiment 1, isolation and identification of bacterial strain

[0156] 1. Isolation and identification of strain W11025

[0157] 1. Isolation of strain W11025

[0158] Add 10.0g of the sample collected from the compost (in Wuyuan County, Inner Mongolia in October 2014) into 90mL sterile water with glass beads, let it stand for 20min, shake it fully on a rotary shaker at 200r / min for 30min, and obtain 1× 10 1 For the diluted bacterial suspension, use a sterile pipette to draw 5.0mL of the above bacterial suspension into a triangular flask filled with 45mL sterile water, shake well to obtain 1×10 2 Dilution of bacteria suspension, according to this method to make 1 × 10 3 , 1×10 4 , 1×10 5 diluted bacterial suspension.

[0159] Under sterile conditions, take 100 μL of the above dilutions of the bacterial suspension and spread it on the beef extract peptone medium, incubate at 20°C for 24-72 hours, observe whether there are colonies, and select a strain that grows f...

Embodiment 2

[0274] Embodiment 2, preparation and application of low temperature resistant composting starter

[0275] 1. Preparation of low temperature resistant composting starter

[0276] 1. Preparation of composite microbial agent

[0277] Take each microbial inoculum according to the following weight and mix to obtain the composite microbial inoculum: 1.4kg of Saccharomyces cerevisiae inoculum, 1.2kg of Candida maltosa inoculum, 1.0kg of Bacillus subtilis inoculum, and 1.4 kg of Bacillus amyloliquefaciens inoculum. kg, Aspergillus oryzae inoculum 1.5kg, Aspergillus niger inoculum 1.3kg, Bacillus stearothermophilus inoculum 1.0kg, Dispospora thermophilic inoculum 1.0kg.

[0278] The preparation method of the above-mentioned Saccharomyces cerevisiae inoculum is as follows: inoculate Saccharomyces cerevisiae W052502 strain (preservation number is CGMCC No.12701) in YPD medium, at 29 ° C, cultivated under the condition of 200rmp for 20 hours, then 3000r / m After being centrifuged and con...

Embodiment 3

[0300] Embodiment 3, preparation and application of low temperature resistant composting starter

[0301] 1. Preparation of low temperature resistant composting starter

[0302] 1. Preparation of composite microbial agent

[0303] Take each microbial inoculum according to the following weight and mix to obtain a composite microbial inoculum: 1.2 kg of Saccharomyces cerevisiae inoculum, 1.3 kg of Candida maltosa inoculum, 0.8 kg of Bacillus subtilis inoculum, and 1.4 kg of Bacillus amyloliquefaciens inoculum. kg, Aspergillus oryzae inoculum 1.5kg, Aspergillus niger inoculum 1.3kg, Bacillus stearothermophilus inoculum 0.8kg, Disposporum thermophilic inoculum 0.8kg. The effective viable counts of each bacterial agent before mixing are: Saccharomyces cerevisiae 5 billion cfu / g, Candida maltosa 5 billion cfu / g, Bacillus subtilis 30 billion cfu / g, Bacillus amyloliquefaciens 50 billion cfu / g, Aspergillus oryzae 2 billion cfu / g, Aspergillus niger 2 billion cfu / g, Bacillus stearothe...

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Abstract

The invention discloses a low-temperature-resistant composting fermentation microbial inoculum with a deodorizing function and applications of the low-temperature-resistant composting fermentation microbial inoculum. The microbial flora contained in the low-temperature-resistant composting fermentation microbial inoculum provided by the invention can tolerate low temperature, and can normally grow at 15-20 DEG C, so that the fermentation air temperature of organic materials in the low-temperature environment is facilitated, in addition, the strains have the mutual promotion effects, therefore, a mutual synergistic effect is generated, and further, decomposing is accelerated. The experiment proves that during the composting process, after the low-temperature-resistant composting fermentation microbial inoculum is added into the organic materials for fermentation, the sum of effective microbes at the composting initial stage is improved, the temperature increase rate of the composted materials is accelerated, the anaerobic fermentation time is shortened, the decomposing process is accelerated, meanwhile, the discharge of sulfur-containing compounds and nitrogen-containing compounds is reduced, the physicochemical properties of the fermentation products are optimized, and in addition, the low-temperature-resistant composting fermentation microbial inoculum has a certain deodorizing function.

Description

technical field [0001] The invention belongs to the field of composite microbial bacterial agents, and in particular relates to a low-temperature-resistant composting fermentation bacterial agent with deodorizing function and application thereof. Background technique [0002] my country produces a huge amount of agricultural organic waste. It is estimated that 3 billion tons of livestock and poultry manure and more than 700 million tons of crop straw are produced every year. These organic wastes are rich in nutrients needed for a large number of crop production. Through the method of aerobic composting, these organic wastes can be turned into high-quality organic fertilizers. The traditional composting method requires a certain amount of time to reproduce due to the small number of effective microorganisms in the initial stage. The fermentation time is long, the odor is large, and the loss of nutrients is large , serious waste of resources and other issues, especially in aut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20C12N1/14C05G3/00C05F11/08C05F17/00B01D53/84B01D53/52B01D53/50B01D53/56B01D53/58C12R1/865C12R1/645C12R1/125C12R1/07C12R1/69C12R1/685
CPCB01D53/50B01D53/52B01D53/56B01D53/58B01D53/84C05D9/00C05F11/08C05F17/00C05G3/00C12N1/14C12N1/16C12N1/20C05F3/00C05F5/00Y02A50/20Y02W30/40
Inventor 武凤霞刘善江刘建斌
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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