Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof

A yolk antibody and infectivity technology, which is applied in the field of mixed yolk antibody for prevention and treatment of infectious serositis in ducks and its preparation, can solve the problem that the research on yolk antibody of R. anatipestifer has not yet been reported, and can reduce the use of antibiotics , high protection rate, and the effect of promoting development

Active Publication Date: 2011-09-21
兆丰华生物科技(南京)有限公司北京生物医药科技中心 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most viral diseases, such as Newcastle disease, egg drop syndrome, chicken infectious bursal disease, duck viral hepatitis, etc., have successfully developed yolk antibodies, but the research on yolk antibodies of R. No report

Method used

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  • Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 R. anatipestifer egg yolk antibody

[0031] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0032] Take R. anatipestifer RA1 (serum type I) and RA2 (serum type II) strains and inoculate them in tryptone yeast broth containing 0.5% serum respectively, and place them at 36-37°C for shaking culture for 18 hours to logarithmic growth phase , centrifuge at 3000rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, add analytically pure formaldehyde solution slowly according to 0.3% of the total amount of the bacteria solution, and add it at 36-37 Inactivation at constant temperature for 36 hours;

[0033] (2) Mix the antigens in proportion, and add immune adjuvants;

[0034] Mix the inactivated RA1 and RA2 cell antigens at a ratio of 1:2, add part of them into sterilized Freund's complete adjuvant in an equal...

Embodiment 2

[0040] The preparation of embodiment 2 R. anatipestifer egg yolk antibody

[0041] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0042] Take R. anatipestifer (serum type I) and RA2 (serum type II) strains and inoculate them in tryptone yeast broth containing 0.5% serum respectively, place them at 36-37°C for shaking culture for 18 hours to logarithmic growth phase, Centrifuge at 3000rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, add analytically pure formaldehyde solution slowly according to 0..3% of the total amount of the bacteria solution, and dissolve it at 36 Inactivate for 36 hours at a constant temperature of ~37°C;

[0043] (2) Mix the antigens in proportion, and add immune adjuvants;

[0044] After inactivation, mix RA1 and RA2 cell antigens at a ratio of 1:4, add an equal volume of sterilized propolis, and emulsify to obta...

Embodiment 3

[0050] The preparation of embodiment 3 R. anatipestifer egg yolk antibody

[0051] (1) Pure culture, concentration and inactivation of R. anatipestifer bacterial antigens;

[0052] Take R. anatipestifer (serum type I) and RA2 (serum type II) strains, inoculate them in tryptone yeast broth containing 0.5% serum respectively, and place them at 36-37°C for shaking culture for 18 hours to logarithmic growth phase , centrifuge at 3000rpm for 15 minutes at 4°C, dissolve the precipitate in PBS buffer, then centrifuge for 15 minutes under the same conditions, collect the bacteria, add analytically pure formaldehyde solution slowly according to 0.3% of the total amount of the bacteria solution, and add it at 36-37 Inactivation at constant temperature for 36 hours;

[0053] (2) Mix the antigens in proportion, and add immune adjuvants;

[0054] After inactivation, mix RA1 and RA2 cell antigens at a ratio of 1:6, add an equal volume of sterilized white oil for injection, and emulsify to...

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Abstract

The invention relates to a mixed yolk antibody for preventing and controlling infective oromeningitis and a preparation method thereof, and belongs to the technical field of biological agent preparation and poultry disease prevention and control. The invention takes a main epidemic serotype strain of duck plague pasteurella anatipestifer as an antigen to prepare the duck plague pasteurella anatipestifer hyperimmune yolk antibody. Infection treatment experimental results show that the prepared yolk antibody has the protection ratio for serotype I and serotype II duck plague pasteurella anatipestifer respectively reaching 80 percent and 90 percent. The product provides a new way to the prevention and control of duck infective oromeningitis and can be used for the preparation of veterinary medicines for controlling the disease or be prepared into health care products or feed additives for use. Moreover, the yolk antibody provides a material basis for improving the livestock product quality, reducing medicinal residue and promoting the human food safety.

Description

technical field [0001] The invention belongs to the technical field of biological agent preparation and poultry disease prevention and treatment, and relates to a mixed yolk antibody for preventing and treating duck infectious serositis and a preparation method thereof. Background technique [0002] Riemerella anatipestifer (RA) disease, also known as duck infectious serositis, is one of the most serious infectious diseases to the duck industry. Fibrinous pericarditis, perihepatitis, air sacculitis, caseous salpingitis, conjunctivitis, arthritis, etc. of poultry species have high morbidity and mortality. The disease is caused by R. anatipestifer, which is sensitive to some antibacterial drugs, but it is difficult to cure, especially after the bacteria invade the air sac, it is difficult for the drugs to reach and it is even more difficult to remove. At present, due to unreasonable drug regimens, drug resistance of R. anatipestifer is widespread, and the drug resistance rate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/12C07K16/02C07K1/14A61K9/08A61K39/40A61K39/39A23K1/16A61P31/04A23K10/20A23K20/147A23K40/00A23K50/75
Inventor 陈义锋赵亚荣张渊魁赵建增
Owner 兆丰华生物科技(南京)有限公司北京生物医药科技中心
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