57results about How to "Reduce the cost of immunization" patented technology

The present invention belong to veterinary new biological medicine technology field, relates to porcine circovirus type II (PCV2) inactivated vaccine of and method for preparing same. The vaccine used seed virus is porcine circovirus type II DBN-SX07 strain, the preservation number is CGMCC No 3064, the virus strain is used as antigen preparation of inactivation by alkyl agents and emulsification by adding oil adjuvant. Using the invention provided PCV2 inactivated vaccine to immune pig can generate an uniform and effective protection force-shorting PCV2 infection windows period obviously, and prolong immune duration-reducing times of booster immunization.

The invention provides a porcine pseudorabies virus vaccine composition. The porcine pseudorabies virus vaccine composition contains a porcine pseudorabies virus subunit antigen, or a recombinant Newcastle disease virus, namely a porcine pseudorabies virus vector. The invention further provides the preparation method and application of the vaccine composition. The vaccine composition can effectively prevent related diseases of a porcine pseudorabies virus and related diseases infected by the porcine pseudorabies virus. The combination of immunogenicity antigens in the porcine pseudorabies virus vaccine composition can be induced to generate a synergetic immune effect, thereby having good immune effect, and further lowering the immune usage amount to lower the immunization cost.

The invention provides a vaccine composition containing porcine circovirus-2 antigen (PCV2) and swine flu antigen (SIV). The vaccine composition comprises at least one porcine circovirus-2 antigen of effective dose and at least one swine flue antigen of effective does as well as an adjuvant. The vaccine composition can be used for preventing and treating diseases associated to the porcine circovirus-2 and the swine flu virus diseases.

The invention relates to a polyvalent vaccine composition for porcine, and in particular relates to a vaccine composition capable of resisting infection of porcine circovirus type 2 (PCV 2) and haemophilus parasuis (HPS) at the same time. The vaccine composition comprises at least one PCV2 antigen, at least one HPS, as well as vector, an excipient and an adjuvant available in the field of veterinary medicine. The vaccine composition can be used for preventing and treating PCV2 related diseases and HPS diseases.

The present disclosure provides a PRV gB protein fragment, or a conservative variant or active fragment thereof, the gB protein fragment has a high level of expression, the subunit vaccine antigen prepared from the gB protein fragment has a better immune effect than a subunit vaccine antigen prepared from gB protein. The invention also provides a preparation method of a subunit vaccine by using the gB protein fragment alone, or the gB protein fragment together with gD protein. This vaccine has a simple preparation method and provides excellent protection against disease caused by the porcine pseudorabies virus.

The invention provides a porcine pseudorabies virus vaccine composition which contains a porcine pseudorabies virus subunit antigen or contains recombined newcastle disease viruses- porcine pseudorabies virus carrier. The invention further provides a preparing method and application for the vaccine composition. The vaccine composition can effectively prevent related diseases of porcine pseudorabies viruses and related infectious diseases caused by the porcine pseudorabies viruses. The combination of the immunogenicity antigen in the porcine pseudorabies virus vaccine composition can induce to generate a synergetic immune effect, the immune effect is good, the immunity use amount is further reduced, and the immunity cost is reduced.

The invention relates to a vaccine composition, which contains classical swine fever virus antigen, porcine circovirus type 2 antigen, mycoplasma hyopneumoniae antigen and a vaccine adjuvant. The invention also provides a method for preparing the vaccine composition, a water-soluble vaccine adjuvant is used for preparing a mixture of the porcine circovirus type 2 antigen and mycoplasma hyopneumoniae antigen, and the mixture is taken as a diluent for diluting classical swine fever virus antigen. The vaccine composition has the advantages of simple preparation method, high titer content of the vaccine, and convenient and fast immunization, one time immunization can simultaneously preventing or treating swine fever, porcine circovirus type 2 antigen and mycoplasma hyopneumoniae, immunization cost is reduced, the immunization program is saved, and the application is more economic and reliable.

The invention discloses a bovini Asia 1 / O type foot-and-mouth disease bivalent multi-epitope vaccine and a preparation method and an application thereof and belongs to the field of veterinary biological vaccines. The bovini Asia 1 / O type foot and mouth disease virus compound multi-epitope recombinant antigen is obtained by coupling dominant epitope of epidemic bovini O and Asia 1 type foot-and-mouth disease virus representative strains connected in series in China with a bovini IgG heavy chain constant region. Immune efficacy experiments verify that the bovini Asia 1 / O type foot-and-mouth disease bivalent multi-epitope vaccine prepared by the invention has good immunogenicity, and high level protective antibodies can be generated by the body induced by immune guinea pig or immune bovine. The inoculated vaccine is safe and harmless to immune animals. The vaccine is a novel vaccine which is abroad in prospect, supports matter storage and technical support to prevention and control of bovini Asia 1 / O type foot-and-mouth disease in China, and is of great importance.

The invention provides an antigen composition for preventing and treating secondary infected respiratory system diseases of pigs. The antigen composition comprises at least one porcine reproductive and respiratory syndrome antigen with immune amount, and at leas one haemophilus parasuis antigen with immune amount and an adjuvant, wherein the porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen respectively and independently comprise attenuated live holoantigen, inactivated holoantigen, subunit antigen, recombinant live carrier antigen and DNA (deoxyribonucleic acid) carrier antigen. The antigen composition cannot cause mutual immune interference or influence of two antigen components, and enables the immune effects of porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen to be strengthened, and is convenient in vaccination process and good in safety.

The invention relates to a beta-glucan modified meningitis polysaccharide conjugate vaccine and a preparation method thereof. The preparation method of the beta-glucan modified meningitis polysaccharide conjugate vaccine comprises the following steps: (1) activating meningococcus polysaccharide by cyanogen bromide, and then deriving by adopting adipic dihydrazide; (2) combining a derived meningococcus polysaccharide derivative with carrier protein; (3) activating beta-glucan; and (4) modifying polysaccharide-protein conjugate by the activated beta-glucan. By virtue of the steps, a novel and efficient meningitis polysaccharide conjugate vaccine can be prepared and can be used for preventing infection caused by epidemic cerebrospinal meningitis Neisseria gonorrhoeae.

The invention provides a swine fever and porcine pseudorabies bivalent vaccine. The swine fever and porcine pseudorabies bivalent vaccine contains at least one swine fever virus antigen and at least one porcine pseudorabies virus antigen, wherein the two antigens coordinate well, are excellent in immune effect and can promote each other. The swine fever and porcine pseudorabies bivalent vaccine is simple in preparation method, is convenient and efficient in immunization and has the advantages that immunization cost is reduced, an immunization procedure is simplified and economy and reliability are realized compared with a vaccine which can be used for preventing and treating more than two diseases only when immunization is carried out in steps and at least two injections are taken and an immune method of the vaccine in the prior art. The immune effect of the swine fever and porcine pseudorabies bivalent vaccine is better than that of a single vaccine and better in safety and avoids adverse effects caused by multiple immunizations. Besides, the invention also provides a simple testing method for determining swine fever effect in the bivalent vaccine by adopting an indirect immunofluorescence method, so that quality of bivalent live vaccines in each batch is guaranteed, and economic benefit is obviously increased.

The invention discloses a bacterial polysaccharides and protein binding combined vaccine for human, wherein Group A and Group B epidemic cerebrospinal meningitis Neisser's coccus capsular polysaccharides and Type B haemophilus influenzae capsular polysaccharide are bonded on an effective protein carrier with covalent bonds by a chemical method to prepare a preventive combined vaccine bonding polysaccharides and protein for human. Three vaccinations are conducted on children with the age of three to eight months and each for one month, and one vaccination is conducted on children older than one year. More than 96.5 percent of vaccine inoculators can be immunized from the Group A and Group C epidemic cerebrospinal meningitis Neisser's coccus and 91.2 percent of the vaccine inoculators can obtain long-term immunity from the Type B haemophilus influenzae one month after immunization. The vaccine is applied to the prevention of the infection caused by the Group A and Group C epidemic cerebrospinal meningitis Neisser's coccus and the Type B haemophilus influenzae.

The invention provides a preparation method of a bi-combined inactivated vaccine. The prepared bi-combined inactivated vaccine provided by the invention can be used for preventing swine type O foot-and-mouth diseases and porcine circovirus type 2. The preparation method provided by the invention is simple, and the vaccine is high in antigen and is convenient to immunize; compared with graded immunization in the prior art, the immunization times can be reduced, the stress reaction can be reduced, and the immunization cost can be lowered; and secondly, comparisons between the effects of immunizing piglets by using a bi-combined vaccine and a monovalent vaccine show that two antigen components cannot interfere with each other in the same injection of the bi-combined vaccine, and moreover, the immune effect of injecting the bi-combined vaccine on the piglets for a single time is higher than the immune effect of sequentially injecting the two vaccines.

The invention relates to a multi-vaccine composition for pigs, in particular to a vaccine composition resisting porcine circovirus type 2 (PCV2) infection and haemophilus parasuis (HPS) infection at the same time. The vaccine composition is prepared from at least one kind of a porcine circovirus type 2 antigen, at least one kind of a haemophilus parasuis antigen, carriers, excipients and adjuvants, wherein the carriers, the excipients and the adjuvants can be obtained in the veterinary medicine field. The vaccine composition can be used for preventing and treating porcine circovirus type 2 related diseases and haemophilus parasuis diseases.

The invention discloses a tilapia mossambica streptococcus agalactiae oral attenuated freeze-dried vaccine and its preparation method. According to the invention, tilapia mossambica streptococcus agalactiae attenuated bacterial strain YM001 is performed with steps of enlarge cultivation, thalline collection and freeze-drying preparation to obtain the oral attenuated vaccine. The immunizing dose, immunization program and a vaccine usage method of the vaccine can be determined through tests. The immune efficacy, security and maneuverability of the vaccine can be detected and confirmed through field tests, the result shows that the vaccine has the advantages of high protection effectiveness, good security, convenient usage and low cost, the vaccine can be used for preventing tilapia mossambica streptococcus agalactiae, and has high business value.

The invention relates to a porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and a preparation method thereof. The bivalent vaccine is a lyophilized vaccine, and comprises a porcine circovirus type 2 SH strain inactivated virus and a respiratory syndrome virus R98 strain live virus, while an adjuvant is added into the vaccine diluent, so that the immunizing effect of the vaccine can be further improved. Immune interference does not exist among the bivalent lyophilized vaccine antigens, and the quality inspection of the vaccine finished product can achieve the quality standard of a single vaccine, and the immune protection effect of the vaccine finished product is better than that of a single vaccine; the bivalent vaccine can be used for simplifying the immune procedure, reducing immune cost and reducing the stress reaction of an immunized pig, and thus the bivalent vaccine has great market application values.

The invention provides a vaccine composition, which includes an immune amount of a mycoplasma hyopneumoniae antigen, an immune amount of a swine influenza virus antigen and an immune amount of a porcine reproductive and respiratory syndrome virus antigen. All antigens of the vaccine composition not only do not generate mutual interference or influence of antigen components, but have the effect of mutually enhancing the immune effect instead. One immunization can achieve the immune effect and the antibody continuous level of single antigen twice immunization. Also, the vaccine composition has the advantages of good security, simple preparation method, convenient and fast immunization, and reduction of the immunization cost, etc.

The invention relates to a bivalent vaccine composition resisting type-2 porcine circovirus and porcine infectious pleuropneumonia infection and a preparation method. The bivalent vaccine composition comprises a type-2 porcine circovirus antigen and an actinobacillus pleuropneumoniae antigen; the immune procedure is simple; the type-2 porcine circovirus related diseases and porcine infectious pleuropneumonia can be effectively prevented at the same time. Compared with a single vaccine, the bivalent vaccine composition does not generate an immune interference phenomenon while the immune effect is not reduced; moreover, the actinobacillus pleuropneumoniae can enhance the immune effect of the type-2 porcine circovirus antigen. The preparation process of the bivalent vaccine composition is simple, the immune cost is low, and the practicability is high.

The invention relates to a mink hemorrhagic pneumonia and botulism combined inactivate vaccine and a preparing method thereof.Strain WD005, strain DL007 and strain ZC118 of pseudomonas aeruginosa for vaccine manufacturing and detecting are obtained through clinical isolation, and the strains are higher in pertinence and more comprehensive in protection for current mink hemorrhagic pneumonia epidemic serotype; by means of virulence tests and immunogenicity tests, the immunogenicity is good; a strain C-type clostridium botulinum C62-4 has the advantage of being superior in immunogenicity.The combined inactivate vaccine can prevent attack of G-type pseudomonas aeruginosa, B-type pseudomonas aeruginosa, C-type pseudomonas aeruginosa and C-type clostridium botulinum to minks at the same time, and has the advantages that the number of inoculation times is reduced, and using is convenient.The labor intensity of immunization is relieved, the immune cost is reduced, the stress reaction of animals is reduced, and the vaccine is more economical and reliable.

The invention provides a Mycoplasma hyorhinis strain LYH, and a vaccine composition prepared from the Mycoplasma hyorhinis strain LYH, in particular to a vaccine composition comprising the Mycoplasma hyorhinis and Mycoplasma hyopneumoniae. The vaccine composition can be effective in prevention and treatment of swine enzootic pneumonia caused by Mycoplasma hyorhinis, Mycoplasma hyopneumoniae single infection or mixed infection. Especially in the circumstance of mixed infection, immune effect of the vaccine composition significantly exceeds that of each single vaccine.

The invention belongs to the technical field of vaccine, and more specifically relates to a porcine pseudorabies virus and porcine circovirus type II bivalent vaccine, and applications thereof. The porcine pseudorabies virus and porcine circovirus type II bivalent vaccine comprises inactivated porcine pseudorabies virus XF-1 strain, and porcine circovirus type II-WH strain. It is confirmed by the porcine pseudorabies virus and porcine circovirus type II bivalent inactivated vaccine that XF-1 strain can be prepared into combined vaccines used for preventing two or more than two diseases with other well-known antigens in the field of vaccine, a cognition error that concurrent infection of porcine circovirus type II and porcine pseudorabies virus is caused is eliminated, combination application of porcine circovirus type II and porcine pseudorabies virus at an appropriate rate is capable of achieving excellent effect, and vaccine development prospect is promising.

The invention provides three porcine contagious actinobacillus pleuropneumoniae strains, two streptococcus suis strains; and based on the discovery that the mutual interference among the antigens is reduced to lowest by cooperatively using multiple antigens, the invention provides a porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine with good immunogenicity. The vaccine employs inactivated porcine contagious actinobacillus pleuropneumoniae type 1, type 5 and type 7 and streptococcus suis as antigens, and has super-powerful virulence and immunogenicity. During preparation process of the vaccine, relatively less antigens are used and the vaccine can reach relatively good immunization effect, and thus the production cost and the usage cost are reduced, and the preparation method is applicable to large-scale production and the vaccine is applicable to culturing farms. The porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine realize the effect of preventing two diseases by using the one vaccine in the level of same production cost and same sale price with a single vaccine, and is capable of replacing the single vaccine for use, and thus the immunization cost is substantially reduced, and the combined vaccine has success potential on business.

The invention discloses a multivalent combined vaccine. Each dosage of the multivalent combined vaccine comprises: 15-20 mu g of group-A epidemic neisseria meningitides capsular polysaccharide, 15-20 mu g of group-C epidemic neisseria meningitides capsular polysaccharide, 15-20 mu g of group-Y epidemic neisseria meningitides capsular polysaccharide, 15-20 mu g of group-W135 epidemic neisseria meningitides capsular polysaccharide, 10-20 mu g of group-B meningococcal attenuated live vaccine and 10-20 mu g of measles attenuated live vaccine. The multivalent combined vaccine helps to reduce immunization injection times, reduce immunization cost and increase acceptability of the vaccine; the multivalent combined vaccine helps to provide research direction for satisfying infant demands, and is expected to alleviate infant pain and parent spirit burden; and by utilizing the synergic effects of different vaccines, the multivalent combined vaccine helps to reduce the occurrence probability of bacteremia, enhance the immunogenicity of group-B meningococcal LOS vaccines, or enhance the immunization effect and prolong the immunization time of group-B meningococcal outer membrane protein.

The invention relates to construction of rough type brucella of a recombinant fine-granule echinococcus Eg95 gene and a vaccine of the rough type Brucella. A rough type cattle-species Brucella low virulent strain RA343 is used as a parent strain, cane sugar suicide plasmid is used as a carrier, the fine-granule echinococcus Eg95 gene containing a specific promoter sequence is optimized through a codon, then the optimized fine-granule echinococcus Eg95 gene is inserted in a brucella genome in a traceless manner, and a recombinant brucella RA343-Eg95 strain which can efficiently express the recombinant fine-graunle echinococcus Eg95 gene is successfully constructed. The recombinant strain not only reserves rough type characteristics of an original parent strain RA343 and has favorable immunoprotection properties on brucellemia (brucellosis); and besides, after animals are immunized with the recombinant strain, antibodies in accordance with fine-granule echinococcus Eg95 can be generated,so that immunoprotection for echinococcosis granulosa can be realized. After the animals are immunized with the vaccine prepared from the recombinant strain, double immunoprotection for the brucellosis and the echinococcosis granulosa can be realized at the same time.

The invention relates to a vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and a preparation method. The vaccine composition comprises pig mycoplasma hyopneumoniae antigens and pig actinobacillus pleuropneumoniae antigens, is simple in immunization procedure, not only is capable of generating antibodies, but also has the functions of toxicity attack protection and effectively controlling pig mycoplasma pneumonia and infectious pleuropneumonia. The vaccine composition has the immunization effect same to an independently injected vaccine, is small in side reaction, long in immunization period, less in consumed time, less in consumed labor, small in encroaching on a pig. The vaccine composition is simple in production technology, low in immunization cost and strong in practicality.

The invention relates to the field of biotechnology, in particular to bivalent subunit vaccines of type 2b and 2d porcine circoviruses and preparation methods thereof. The invention provides recombinant baculoviruses comprising one or more copies of a Cap protein encoding gene of type 2b or 2d porcine circovirus. The invention also provides the bivalent subunit vaccines of the type 2b and 2d porcine circoviruses which comprise Cap proteins of the type 2b and 2d porcine circoviruses expressed by the recombinant baculoviruses respectively. Through artificial codon optimization, high-level and high-purity expression of the Cap proteins of the type 2b and 2d porcine circovirus is achieved, and the natural structure and immunogenicity of the Cap proteins is ensured. The bivalent subunit vaccines of the type 2b and 2d porcine circoviruses have high immunogenicity and safety, and exert an excellent immunoprotective effect on the porcine circoviruses.

The invention provides a vaccine composition containing a swine mycoplasmal pneumonia antigen and a swine streptococcosis antigen, and a preparation method and an application thereof. The vaccine composition includes an immunizing dose of the swine mycoplasmal pneumonia antigen, an immunizing dose of the swine streptococcosis antigen, and an adjuvant. The vaccine composition has a simple immunization program, can effectively control the swine mycoplasmal pneumonia antigen and the swine streptococcosis antigen, has an immune effect equivalent to the immune effect realized through respective injection of single vaccines, and also has the characteristics of small side reaction, long immune period, less time consumption, and less labor consumption; and the vaccine composition also has the advantages of simple production technology, low immune cost and strong practicality.