56results about How to "Reduce the cost of immunization" patented technology

The present invention belong to veterinary new biological medicine technology field, relates to porcine circovirus type II (PCV2) inactivated vaccine of and method for preparing same. The vaccine used seed virus is porcine circovirus type II DBN-SX07 strain, the preservation number is CGMCC No 3064, the virus strain is used as antigen preparation of inactivation by alkyl agents and emulsification by adding oil adjuvant. Using the invention provided PCV2 inactivated vaccine to immune pig can generate an uniform and effective protection force-shorting PCV2 infection windows period obviously, and prolong immune duration-reducing times of booster immunization.

The invention provides a porcine pseudorabies virus vaccine composition. The porcine pseudorabies virus vaccine composition contains a porcine pseudorabies virus subunit antigen, or a recombinant Newcastle disease virus, namely a porcine pseudorabies virus vector. The invention further provides the preparation method and application of the vaccine composition. The vaccine composition can effectively prevent related diseases of a porcine pseudorabies virus and related diseases infected by the porcine pseudorabies virus. The combination of immunogenicity antigens in the porcine pseudorabies virus vaccine composition can be induced to generate a synergetic immune effect, thereby having good immune effect, and further lowering the immune usage amount to lower the immunization cost.

The invention provides a vaccine composition containing porcine circovirus-2 antigen (PCV2) and swine flu antigen (SIV). The vaccine composition comprises at least one porcine circovirus-2 antigen of effective dose and at least one swine flue antigen of effective does as well as an adjuvant. The vaccine composition can be used for preventing and treating diseases associated to the porcine circovirus-2 and the swine flu virus diseases.

The invention relates to a polyvalent vaccine composition for porcine, and in particular relates to a vaccine composition capable of resisting infection of porcine circovirus type 2 (PCV 2) and haemophilus parasuis (HPS) at the same time. The vaccine composition comprises at least one PCV2 antigen, at least one HPS, as well as vector, an excipient and an adjuvant available in the field of veterinary medicine. The vaccine composition can be used for preventing and treating PCV2 related diseases and HPS diseases.

The invention provides an antigen composition for preventing and treating secondary infected respiratory system diseases of pigs. The antigen composition comprises at least one porcine reproductive and respiratory syndrome antigen with immune amount, and at leas one haemophilus parasuis antigen with immune amount and an adjuvant, wherein the porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen respectively and independently comprise attenuated live holoantigen, inactivated holoantigen, subunit antigen, recombinant live carrier antigen and DNA (deoxyribonucleic acid) carrier antigen. The antigen composition cannot cause mutual immune interference or influence of two antigen components, and enables the immune effects of porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen to be strengthened, and is convenient in vaccination process and good in safety.

The invention relates to a porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and a preparation method thereof. The bivalent vaccine is a lyophilized vaccine, and comprises a porcine circovirus type 2 SH strain inactivated virus and a respiratory syndrome virus R98 strain live virus, while an adjuvant is added into the vaccine diluent, so that the immunizing effect of the vaccine can be further improved. Immune interference does not exist among the bivalent lyophilized vaccine antigens, and the quality inspection of the vaccine finished product can achieve the quality standard of a single vaccine, and the immune protection effect of the vaccine finished product is better than that of a single vaccine; the bivalent vaccine can be used for simplifying the immune procedure, reducing immune cost and reducing the stress reaction of an immunized pig, and thus the bivalent vaccine has great market application values.

The invention provides a vaccine composition, which includes an immune amount of a mycoplasma hyopneumoniae antigen, an immune amount of a swine influenza virus antigen and an immune amount of a porcine reproductive and respiratory syndrome virus antigen. All antigens of the vaccine composition not only do not generate mutual interference or influence of antigen components, but have the effect of mutually enhancing the immune effect instead. One immunization can achieve the immune effect and the antibody continuous level of single antigen twice immunization. Also, the vaccine composition has the advantages of good security, simple preparation method, convenient and fast immunization, and reduction of the immunization cost, etc.

The invention relates to a mink hemorrhagic pneumonia and botulism combined inactivate vaccine and a preparing method thereof.Strain WD005, strain DL007 and strain ZC118 of pseudomonas aeruginosa for vaccine manufacturing and detecting are obtained through clinical isolation, and the strains are higher in pertinence and more comprehensive in protection for current mink hemorrhagic pneumonia epidemic serotype; by means of virulence tests and immunogenicity tests, the immunogenicity is good; a strain C-type clostridium botulinum C62-4 has the advantage of being superior in immunogenicity.The combined inactivate vaccine can prevent attack of G-type pseudomonas aeruginosa, B-type pseudomonas aeruginosa, C-type pseudomonas aeruginosa and C-type clostridium botulinum to minks at the same time, and has the advantages that the number of inoculation times is reduced, and using is convenient.The labor intensity of immunization is relieved, the immune cost is reduced, the stress reaction of animals is reduced, and the vaccine is more economical and reliable.

The invention provides a Mycoplasma hyorhinis strain LYH, and a vaccine composition prepared from the Mycoplasma hyorhinis strain LYH, in particular to a vaccine composition comprising the Mycoplasma hyorhinis and Mycoplasma hyopneumoniae. The vaccine composition can be effective in prevention and treatment of swine enzootic pneumonia caused by Mycoplasma hyorhinis, Mycoplasma hyopneumoniae single infection or mixed infection. Especially in the circumstance of mixed infection, immune effect of the vaccine composition significantly exceeds that of each single vaccine.

The invention provides three porcine contagious actinobacillus pleuropneumoniae strains, two streptococcus suis strains; and based on the discovery that the mutual interference among the antigens is reduced to lowest by cooperatively using multiple antigens, the invention provides a porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine with good immunogenicity. The vaccine employs inactivated porcine contagious actinobacillus pleuropneumoniae type 1, type 5 and type 7 and streptococcus suis as antigens, and has super-powerful virulence and immunogenicity. During preparation process of the vaccine, relatively less antigens are used and the vaccine can reach relatively good immunization effect, and thus the production cost and the usage cost are reduced, and the preparation method is applicable to large-scale production and the vaccine is applicable to culturing farms. The porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine realize the effect of preventing two diseases by using the one vaccine in the level of same production cost and same sale price with a single vaccine, and is capable of replacing the single vaccine for use, and thus the immunization cost is substantially reduced, and the combined vaccine has success potential on business.

The invention relates to construction of rough type brucella of a recombinant fine-granule echinococcus Eg95 gene and a vaccine of the rough type Brucella. A rough type cattle-species Brucella low virulent strain RA343 is used as a parent strain, cane sugar suicide plasmid is used as a carrier, the fine-granule echinococcus Eg95 gene containing a specific promoter sequence is optimized through a codon, then the optimized fine-granule echinococcus Eg95 gene is inserted in a brucella genome in a traceless manner, and a recombinant brucella RA343-Eg95 strain which can efficiently express the recombinant fine-graunle echinococcus Eg95 gene is successfully constructed. The recombinant strain not only reserves rough type characteristics of an original parent strain RA343 and has favorable immunoprotection properties on brucellemia (brucellosis); and besides, after animals are immunized with the recombinant strain, antibodies in accordance with fine-granule echinococcus Eg95 can be generated,so that immunoprotection for echinococcosis granulosa can be realized. After the animals are immunized with the vaccine prepared from the recombinant strain, double immunoprotection for the brucellosis and the echinococcosis granulosa can be realized at the same time.

The invention relates to a vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and a preparation method. The vaccine composition comprises pig mycoplasma hyopneumoniae antigens and pig actinobacillus pleuropneumoniae antigens, is simple in immunization procedure, not only is capable of generating antibodies, but also has the functions of toxicity attack protection and effectively controlling pig mycoplasma pneumonia and infectious pleuropneumonia. The vaccine composition has the immunization effect same to an independently injected vaccine, is small in side reaction, long in immunization period, less in consumed time, less in consumed labor, small in encroaching on a pig. The vaccine composition is simple in production technology, low in immunization cost and strong in practicality.