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Porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine and preparation method thereof

A dual inactivated vaccine and pleuropneumonia technology, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve problems such as losses and no countermeasures

Active Publication Date: 2014-02-12
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the large number of porcine pleuropneumonia serotypes and types of Streptococcus suis, there are no effective countermeasures for the co-infection and simultaneous onset of the two diseases, often causing huge losses

Method used

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  • Porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine and preparation method thereof
  • Porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine and preparation method thereof
  • Porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation of embodiment 1 porcine infectious pleuropneumonia, Streptococcus suis dual inactivated vaccine

[0049] 1. Strains

[0050] Actinobacillus pleuropneumoniae serotype 1 LC strain, isolated and identified by Pulaike Bioengineering Co., Ltd., has been preserved in the China Center for Typical Cultures. The preservation date: December 9, 2011, and the preservation number is : CCTCC M 2011458.

[0051] The actinobacillus pleuropneumoniae serotype 2 XT strain is disclosed in Chinese patent CN102391975A, and the preservation number is: CCTCC NO M 2011410.

[0052] Actinobacillus pleuropneumoniae serotype 5 YC strain, isolated and identified by Pulaike Bioengineering Co., Ltd., has been preserved in the China Center for Typical Cultures. The preservation date: December 9, 2011, and the preservation number is : CCTCC M2011459.

[0053] Actinobacillus pleuropneumoniae serotype 7 YS strain, isolated and identified by Pulaike Bioengineering Co., Ltd., has been pr...

Embodiment 2

[0103] Embodiment 2 Porcine infectious pleuropneumonia, streptococcus suis dual inactivated vaccine comparative test

[0104] At present, the inactivated porcine infectious pleuropneumonia vaccines on the market are mainly produced by Wuhan Keqian and China Animal Husbandry Chengdu Pharmaceutical Equipment Factory, and the Streptococcus suis vaccines are mainly produced by Wuhan Keqian, Shanghai Haili, Guangdong Yongshun and Shandong Huahong. . For the vaccines produced by the above companies, we used the same preparation method in this study to prepare several dual inactivated vaccines with the same serotype as the commercially available products, and compared these dual inactivated vaccines. Then, a series of comparisons were made between the optimal dual vaccine obtained after the comparison and the products of the above-mentioned companies sold in the market. Among them, the detailed information of relevant porcine infectious pleuropneumonia vaccines and streptococcal vac...

Embodiment 3 2

[0169] Embodiment 3 dual vaccine and single vaccine combined use comparative test

[0170] Combined with the test results of Example 2, it can be seen that the porcine infectious pleuropneumonia and Streptococcus suis dual inactivated vaccine prepared by this research method can produce effective protection for piglets and pregnant sows. In this example, the optimal combination of porcine infectious pleuropneumonia (serum type 1 LC strain + serum type 5 YC strain + serum type 7 YS strain), Streptococcus suis (Streptococcus equi subspecies zooepidemicus + Streptococcus suis type 2 SC strain) ) dual inactivated vaccine and a commercially available single vaccine were used in combination to conduct an effect comparison test.

[0171] 1. Vaccine preparation and acquisition

[0172] 1.1 Porcine infectious pleuropneumonia (serum type 1 LC strain + serum type 5 YC strain + serum type 7 YS strain), Streptococcus suis (Streptococcus equi subspecies zooepidemicus + Streptococcus suis t...

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Abstract

The invention provides three porcine contagious actinobacillus pleuropneumoniae strains, two streptococcus suis strains; and based on the discovery that the mutual interference among the antigens is reduced to lowest by cooperatively using multiple antigens, the invention provides a porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine with good immunogenicity. The vaccine employs inactivated porcine contagious actinobacillus pleuropneumoniae type 1, type 5 and type 7 and streptococcus suis as antigens, and has super-powerful virulence and immunogenicity. During preparation process of the vaccine, relatively less antigens are used and the vaccine can reach relatively good immunization effect, and thus the production cost and the usage cost are reduced, and the preparation method is applicable to large-scale production and the vaccine is applicable to culturing farms. The porcine contagious pleuropneumonia and streptococcus suis disease combined inactivated vaccine realize the effect of preventing two diseases by using the one vaccine in the level of same production cost and same sale price with a single vaccine, and is capable of replacing the single vaccine for use, and thus the immunization cost is substantially reduced, and the combined vaccine has success potential on business.

Description

technical field [0001] The invention relates to a porcine infectious pleuropneumonia and streptococcus suis dual inactivated vaccine and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Porcine infectious pleuropneumonia is an important respiratory infectious disease caused by Actinobacillus pleuropneumoniae (APP). This disease mainly causes a kind of fibrinous, hemorrhagic, and necrotizing pneumonia accompanied by pleurisy in pigs. Most of them are the most acute or acute course and die quickly. The morbidity and mortality are more than 50%. It can occur at any age. Pigs, especially in intensive pig farms, will cause significant economic losses once it occurs. [0003] Actinobacillus pleuropneumoniae, also known as Haemophilus parahaemolyticus (H.parahaemolyties), is a Gram-negative coccobacillus with strict growth requirements. There are many serotypes of this pathogen, which can be divided into biological type I ...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61K39/02A61K39/116A61P31/04C12N1/20
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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