The invention relates to the technical field of veterinary biological products, in particular to a quadruple inactivated vaccine of mycoplasma pneumoniae and haemophilus parasuis, streptococcus suis,and actinobacillus pleuropneumoniae. The quadruple inactivated vaccine comprises inactivated mycoplasma hyopneumoniae strain HNMhy1 serotype antigen, inactivated haemophilus parasuis strain HNHPS1 serotype antigen, inactivated haemophilus parasuis strain HN1570 serotype antigen, inactivated streptococcus suis strain HNSS1 serotype antigen, inactivated actinobacillus pleuropneumoniae strain HNAPP1serotype antigen, and an immune adjuvant, the five strains have good immunogenicity and can prevent the infection of clinical mycoplasma pneumoniae and haemophilus parasuis, streptococcus suis, and actinobacillus pleuropneumoniae, the quadruple inactivated vaccine has good safety, good immune effect and long immunization period, can achieve a purpose of preventing various diseases by one-time injection, and reduces costs.