3 results about "Pichia anomala" patented technology

This invention discloses a method for enhancing the antibacterial activity of Propionibacterium freudenreichii subsp. shermanii metabolites by adding a surfactant to a culture medium. The method uses Propionibacterium freudenreichii subsp. shermanii NBRC No. 12426 as the experimental strain and Pichia anomala SICC 2.894 as the indicator strain. A surfactant is added to the fermentation medium to enhance the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii against the indicator strain Pichia anomala. Based on the culture conditions and method of Propionibacterium freudenreichii subsp. shermanii in this invention, the optimal concentration of the surfactant Tween-80 is selected as 0.3%, which significantly enhances the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii.

The application provides a kind of sweet sorghum silage special compound microbial inoculant based on heavy ion beam irradiation breeding, and relates to the technical field of microbial breeding and silage feed, which is composed of Lactobacillus citreum mutant strain Lc15 and Pichia anomala mutant strain Pa22, and the inoculation ratio of the two strains in the compound microbial inoculant is 1:1 (volume ratio). The application has the following advantages: high acid production efficiency; the lactic acid production of Lc15 in the compound microbial inoculant is further improved compared with the original strain, the lactic acid production of the compound system is further improved compared with the original strain combination, and the pH value of the silage system can be quickly reduced; good synergistic performance; Pa22 and Lc15 are metabolically synergistic, which moderately increases the acetic acid content while improving the lactic acid production, thereby enhancing the aerobic stability and avoiding affecting the palatability; good genetic stability; the two mutant strains and the compound system have no significant fluctuation in acid production performance after being continuously passed for 9 times, which meets the demand of large-scale application.

This invention discloses a method and its application for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites by adding carrageenan to a semi-solid culture medium. The method uses *Propionibacterium f. reudenreichii* subsp. *shermanii* NBRC No. 12426 as the experimental strain and *Pichia anomala* SICC 2.894 as the indicator strain. It discloses a method for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites against the indicator strain *Pichia anomala* by adding carrageenan to the culture medium. Based on the culture conditions and method of *Propionibacterium f. reudenreichii* subsp. *shermanii* in this invention, the optimal amount of carrageenan added is selected as 0.6%, which significantly enhances the antibacterial activity of the *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites.