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Pichia anomala strain capable of directly preparing xylose into alcohol

A technology of abnormal Pichia pastoris and Pichia pastoris, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., to achieve the effect of reducing costs, reducing production costs, and broadening the source of raw materials

Inactive Publication Date: 2012-07-04
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiency that the current alcohol producing strains—Saccharomycescerevisiae (non-engineering bacteria) cannot directly use xylose to ferment alcohol, and the ability of xylose engineering strains to utilize xylose can not meet the needs of industrialization, and provide a strain Pichia anomaly strains that can directly use xylose to produce alcohol, thereby expanding the source of raw materials for the production of ethanol by the current ethanol production strains, and reducing the cost of ethanol production from cellulose

Method used

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  • Pichia anomala strain capable of directly preparing xylose into alcohol
  • Pichia anomala strain capable of directly preparing xylose into alcohol

Examples

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Effect test

Embodiment 1

[0021] First, the cellulose raw material was processed into a xylose solution as a carbon source, added to the normal medium for yeast alcohol fermentation, adjusted to pH 4.0, and used.

[0022] Secondly, alcoholic fermentation: the MLB1 strain was cultured in sterilized xylose liquid medium (sugar concentration of 2%) according to the conventional liquid seed culture method of microbial fermentation. When the number of yeast bacteria in the culture solution is more than 1×108, put it into the fermenter for 1 / 15 of the fermenter culture solution (sterilized, with a sugar concentration of 5%) for cultivation. The temperature is controlled at 28°C and the pH value Control at 4.0, maintain continuous or intermittent ventilation with sterile air, that is, ventilation for 10 minutes every 1 hour. Incubate for 48 hours. 100% of xylose is utilized to produce alcohol, and its yield is greater than 50%.

[0023] Third, distillation: according to the current conventional alcohol prod...

Embodiment 2

[0025] Differences from Example 1: the pH value of the culture solution is 4.5, the sugar concentration of the seed culture solution is 3%, the sugar concentration of the fermentor culture solution is 7%, and the culture time is 60 hours.

Embodiment 3

[0027] Differences from Example 1: the pH value of the culture solution is 5.2, the sugar concentration of the seed culture solution is 5%, the sugar concentration of the fermentor culture solution is 10%, and the culture time is 72 hours.

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Abstract

The invention relates to a Pichia anomala strain capable of directly utilizing xylose, belonging to the technical field of industrial microbial fermentation engineering. The strain MLB1 provided by the invention is separated out from the natural ecological environment in the suburb of Yuanjiang County, Yunnan Province, and is a wild-type strain capable of directly fermenting xylose into alcohol. Taxonomic identification shows that the strain belongs to Pichia anomala, the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.4252. The strain can directly ferment xylose or glucose into alcohol 100%, the alcohol yield based on the xylose carbon source is higher than 50%, and the alcohol conversion rate based on the glucose carbon source is higher than 60%. The Pichia anomala strain capable of directly preparing xylose into alcohol makes up the deficiency of the xylose utilization capability of the xylose engineering strain, thereby widening the source range of alcohol-producing materials of the existing alcohol-producing strain, and lowering the cellulose alcohol-producing cost. Simultaneously, the invention also provides a genetic basis for microzyme-based alcohol fermentation from xylose.

Description

Technical field: [0001] The invention relates to an abnormal Pichia strain capable of directly utilizing xylose to produce alcohol, and belongs to the technical field of industrial microorganism fermentation engineering. Background technique: [0002] Environment, energy and sustainable development have become global problems, among which energy is an important material basis for human society. With the rapid development of modern industry, agriculture and national defense science and technology, the consumption of energy has increased significantly. A full-scale energy crisis has caused mankind to start looking for alternative energy sources. The proportion of renewable biomass resources must be gradually expanded. Ethanol as an alternative fuel has the following advantages: (1) The production cost is relatively low; (2) The pollution to the environment is much smaller than that of gasoline and diesel; (3) 20% ethanol and gasoline are mixed to use, and the car engine does...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/84
Inventor 乔敏贾东晨余泽芬张克勤周薇
Owner YUNNAN UNIV
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