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Abnormal pichia anomala and application thereof

A Pichia pastoris, abnormal technology, applied in the field of microorganisms

Inactive Publication Date: 2014-04-23
北京莱万生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Pichia anomala is widely distributed in nature, but there is no report of Pichia anomala controlling apple gray mold, blue mold and peach brown rot in China

Method used

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  • Abnormal pichia anomala and application thereof
  • Abnormal pichia anomala and application thereof
  • Abnormal pichia anomala and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Isolation and strain identification of Pichia anomala YPL6 of the present invention

[0062] 1. Sample collection

[0063] Fruits are collected from organic apple orchards in China.

[0064] 2. Isolation and screening of strains and antagonistic screening

[0065] Bacteria were isolated from organic fruits, separated by conventional gradient dilution coating, and cultured on PDA (containing streptomycin sulfate) and YPD agar (containing streptomycin sulfate) at 28°C, respectively, and the colonies were picked. Bacteria with large morphological differences and pure culture were purified and stored on YPD agar medium, and the primary screening and repeated screening of antagonistic bacteria were carried out with Botrytis cinerea as the target pathogen, and a strain with strong antibacterial activity was finally obtained. Yeast strain, named YPL6.

[0066] 3. Identification of strains

[0067] The morphological, physiological and biochemical characteristics and some co...

Embodiment 2

[0072] Determination of the antibacterial spectrum of Pichia anomala YPL6 of the present invention

[0073] Tested pathogens:

[0074] Peach brown rot fungus--Monilinia fructicola (wint.) Rehm;

[0075] Botrytis cinerea (Botrytis cinerea Per.ex Fr) after apple harvest;

[0076] Apple postharvest Penicillium--Penicillium expansum Link, 1809;

[0077] Pear postharvest Penicillium--Penicillium expansum Link, 1809;

[0078] The above pathogenic bacteria were selected as indicator fungi, and the bioassay method was used for artificial perforation in the fruit body (taking the control of apple postharvest botrytis as an example to illustrate).

[0079] The specific operations are as follows:

[0080] The artificial perforation inoculation method in the fruit is as follows: the pathogenic bacteria strains are incubated in YPD liquid medium at 28°C for 48h, and the concentration of the bacteria liquid is adjusted to 1×10 with distilled water. 8 cfu / ml. B. cinerea was cultured on...

Embodiment 3

[0086] Determination of the inhibitory effect of Pichia anomala YPL6 on diseases of the present invention

[0087] The effect of YPL6 on postharvest disease control of apples, peaches and pears under storage conditions.

[0088] 1. After picking, the apples are soaked in YPL6 bacterial solution for 30-60 seconds, then air-dried at room temperature, and then stored in the dark at a temperature of 1-2°C and a relative humidity of 90-95%. After 10 months of storage, the control effect was calculated compared with the control group. When the cell concentration is ≥10 8 When cfu / ml, the control effect is more than 80%; when the cell concentration is 10 7 When cfu / ml, the control effect is 60%; when the cell concentration is 10 6 When cfu / ml, the control effect is 50%. The effective concentration is ≥10 7 cfu / ml.

[0089] 2. After picking, the peaches are soaked in YPL6 bacterial solution for 30-60 seconds, then air-dried at room temperature, and then stored in the dark at a te...

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Abstract

The invention discloses an abnormal pichia anomala and an application thereof, and belongs to the technical field of micrograms. The strain of the abnormal pichia anomala disclosed by the invention is YPL6, with the preservation number of CGMCC No.8041. The abnormal pichia anomala YPL6 and a metabolite product of the pichia anomala YPL6 have the inhibition function on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium italicum and pear postharvest penicillium italicum, and have the inhibition function on peach postharvest brown rot, apple postharvest botrytis, pear postharvest penicilliosis and apple postharvest penicilliosis.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Pichia anomaly and its application. Background technique [0002] Plant fungal diseases have brought huge losses to agricultural production, and fruit postharvest fungal diseases are an important type of disease, resulting in a large reduction in fruit production, seriously affecting the edible value and market value of fruits, and resulting in the economic income of fruit farmers. reduce. In developed countries, the losses caused by postharvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by postharvest diseases account for 30% or even 30% of the total amount of fruits. higher up. The occurrence of postharvest fruit diseases has seriously affected modern agricultural production, especially the production and processi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/84
Inventor 全利红
Owner 北京莱万生物技术有限公司
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