44 results about "Penicillium italicum" patented technology

The invention discloses an application of tea saponin and mixture thereof in antisepsis and preservation of fruit, overcomes the shortages of the existing tea saponin in the antispesis and preservation of the fruit, provides the wide application of tea saponin in the antisepsis and preservation technical field of fruit and relates to the antisepsis and preservation application of various fruits and the control effects on post-harvest diseases of fruits such as penicillium italicum of citrus, green mold of citrus, Alternaria citri, banana crown rot, banana anthracnose, litchi blossom blight, lichi anthracnose, loquat anthracnose, loquat black spot or loquat gray spot, and the like. The tea saponin and the mixture thereof have safe use, meet the requirement of agricultural sustainable development, effectively reduce the harm of chemical agents on human being, and provide technical supports for good prospect of the application of the tea saponin and the mixture thereof in the field of antisepsis and preservation of the fruit.

The invention discloses hanseniaspora uvarum; the bacterial strain is Y68; the preservation number is CGMCC No. 8042. The hanseniaspora uvarum Y68 has the inhibiting effect on post-harvest monilinia fructicola of peaches and cherries, post-harvest botrytis cinerea of grapes, tomato botrytis, post-harvest botrytis cinerea of apples, post-harvest penicillium italicum of apples and post-harvest penicillium italicum of pears, and has the inhibiting effect on post-harvest brown rot of peaches, cherry brown rot, grape botrytis, tomato botrytis, post-harvest botrytis of apples, post-harvest penicilliosis of pears and post-harvest penicilliosis of apples. The hanseniaspora uvarum Y68 specifically has obvious inhibiting effect on the botrytis cinerea; the diameters of inhibition zones are all greater than 10 mm.

The invention discloses antibacterial and anti-virus applications of marine bacillus amyloliquefaciens 1A in fruit postpartum pathogenic fungi. The fruit postpartum pathogenic fungi are colletotrichum gloeosporioides, coniella diplodiella, pear alternaria alternata and penicillium italicum. The separating method of the marine bacillus amyloliquefaciens 1A comprises the following steps: collecting seawater, sea mud, marine debris, clam, enteromorpha and mudskipper samples in Lianyungang sea area; carrying out separation and purification of bacteria after enriching, so as to obtain a plurality of pure strains of marine bacteria; determining the inhibiting effects of the pure strains of the marine bacteria on the four fruit postpartum pathogenic fungi for a test by using a plate confrontation method; screening to obtain the strains of which the width of the bacteriostatic band is greater than 5mm; determining the bacteriostatic action of sterile fermented liquid on the four fruit postpartum pathogenic fungi by using an oxford cup method; and screening to obtain the bacillus amyloliquefaciens 1A of which the width of the bacteriostatic band is greater than 10mm. Therefore, the marine bacillus amyloliquefaciens 1A has a broad application prospect.

Methods and compositions comprising essential oils or components thereof for protecting harvested fruits against decay caused by a specific pathogenic fungus are provided, such as Cinnamon cassia oil for protection of persimmons against Alternaria alternata, strawberries against Botrytis cinerea, or mandarins against Penicillium italicum or Penicillium digitatum.

The invention relates to marine streptomyces with antibacterial activity and belongs to the technical field of microbes. The marine streptomyces is streptomyces chumphonensis AM-4, is registered and collected in China General Microbiological Culture Collection Center on October 30, 2017 and has a collection number of CGMCC No.14843. The streptomyces chumphonensis AM-4 has an excellent inhibitory effect on ccolletotrichum glecosporioides, penicillium italicum, penicillium digitatum and pathogen of brown stem-end rot, and has the potential of being developed into a biopesticide.

The invention discloses a dicoumarin derivative and a preparation method and application thereof. The molecular formula of the dicoumarin derivative is C22H18O7, wherein the structural formula is as shown in a formula I. The preparation method of the dicoumarin derivative comprises the following steps: (1) carrying out bacterial culture on aspergillus clavatus (Aspergillus clavatus) R7 in a strain medium; and (2) carrying out fermentation cultivation on the strain obtained in the step (1) in a fermentation medium and then extracting by using an ethanol water solution or a methanol water solution, carrying out vacuum concentration, extracting by using an organic solvent and finally further purifying through silica-gel column chromatography, Sephdex LH-20 gel column chromatography and recrystallization to obtain the dicoumarin derivative. The prepared dicoumarin derivative has very strong effects of inhibiting tomato blight, penicillium italicum and gloeosporium musarum and can be used as a microbial bactericide, the raw materials can be produced on a large scale and the application prospect is wide. The formula is as shown in the specification.

The invention discloses a peganum harmala lipid transfer protein (PHP) and relates to the preparation and use of new protein with antifungal, anticancer and antivirus activities. The method uses seeds of a peganum harmala plant as raw materials and comprises the following steps: preparing a coarse product, performing cationic exchange chromatographic separation and purifying by using a molecular sieve; and gradually separating and collecting a purified component having antibacterial activity, dialyzing, desalting, freeze-drying and thus, obtaining purified PHP. In the invention, a filter paper disc agar diffusion method is adopted, and researches prove the protein has growth inhibiting effect on five plant pathogenic fungi, namely alternaria alternate, penicillium degitatum, rhizopus stuolonifer, magnaporthe grisea and penicillium italicum. An 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method proves that the protein has inhibiting effect on proliferation of human esophageal carcinoma cell Eca109, human cervical cancer cells HeLa, human stomach cancer cells MGC-7, mouse melanoma cells B16 and the like. The protein can inhibit the activity of human immunodeficiency virus 1 (HIV-1) reverse transcriptase. The protein can be used for developing medicines for resisting agricultural pathogenic fungi, tumors and viruses.

The invention relates to benzoxazepin bacteriostats and a preparation method thereof. The chemical structural formula is shown in the specification, wherein substituents R1, R2 and R3 are chloro, bromo, methyl, normal-butyl, p-chlorophenyl, methyl acetate groups, tertiary butyl or cyclohexyl, and positions, the number and conjugate positions of the substituents are not fixed. According to the synthetic method, ortho-azidobenzoic acid compounds and aryl ketoaldehyde compounds and in-situ formed isocyanide compounds are subjected toa Passerini reaction, a formed product and triphenylphosphine are subjected toa Staudinger reaction, and polysubstituted benzoxazepin heterocyclic compounds and other important heterocyclic compounds are produced through ring closing of an intramolecular aza-Wittig reaction. The key point of the invention is to provide one novel synthetic method which adopts fewer steps and has high yield to synthesize the novel benzoxazepin compounds. The compounds have better inhibitory activity on Rhizoctonia solani, Penicillium digitatum and Penicillium italicum and can be used as the bacteriostats.

The invention relates to a method for improving the biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables. The method comprises the following steps of (1) activating biocontrol yeasts; and (2) carrying out liquid culture on the activated biocontrol yeasts. The biocontrol yeasts are marine yeasts Rhodosporidium paludigenum Fell&Tallman, and a strain preservation number is IMI 394084; organic acid is added into a culture medium, so that the capacity of the antagonistic yeasts in preventing and controlling the postharvest acidic pathogenic fungi of the fruits and vegetables is improved; the acidic pathogenic fungi include penicillium digitatum, penicillium italicum, penicillium expansum, botrytis cinerea and sclerotinia; and the method can effectively improve the survival rate of the antagonistic yeasts in different organic acid stresses, improves the capacity of the antagonistic yeasts in preventing and controlling postharvest diseases, is safe, efficient, green and pollution-free, conforms to the natural and environment-friendly concept, and is easy for large-scale application due to the high growth and propagation speed of microorganisms.

The invention discloses a method for extracting and separating a flavonoid compound pinocembrin-7-O-belta-D-glucoside from a hairy fig and application of the pinocembrin-7-O-belta-D-glucoside and belongs to the technical field of botanical pesticides. Particularly the compound is separated with the method of subjecting a hairy fig crude extract to macroporous resin column chromatography, Sephadex LH-20 gel column chromatography and recrystallization, and the compound has good bacteriostatic activity to citrus postharvest pathogen-penicillium italicum and good disease resistance to citrus blue mold and can be used for developing a novel plant source citrus preservative.

The invention discloses dithiocarbamate compounds, and a preparation method and application thereof. The dithiocarbamate compounds disclosed by the invention comprise 5-aminopyrazole-dithiocarbamate,indole-dithiocarbamate and substituted benzene-dithiocarbamate. The dithiocarbamate compounds with structural formulas as shown in a formula I, a formula II and a formula III are respectively preparedthrough reactions of an aromatic hydrocarbon 5-aminopyrazole compound, an indole compound and a substituted benzene compound with an amino dithioformyl disulfide compound in a reaction solvent in thepresence of an oxidizing agent and an iodine reagent. The compounds have different degrees of antibacterial activity on Chinese rose anthracnose, Fusarium oxysporum, Fusarium proliferatum, Fusarium solani, Geotrichum candidum, Penicillium digitatum, Penicillium italicum, Phyricularia oryzae and the like.

The invention relates to a green method for preserving navel oranges at normal temperature. The method comprises the following steps of harvesting, ultrasonic treatment, traditional Chinese medicine extract soaking, air-drying treatment, package, storage and the like. According to the method, navel oranges are soaked by adopting an illicium verum extracting solution, wherein illicium verum is a natural spice, is safe and nontoxic, can be used for inhibiting growth of penicillium italicum wehmer and penicillium digitatum, and is used for warehouse-out preservation of navel oranges, so that therotting rate of navel oranges can be effectively reduced, fruit moisture maintenance of navel oranges can be facilitated, and the shelf life of navel oranges can be prolonged. Package for single navelorange is carried out by adopting a polyethylene preservative seal bag and can be mechanically operated to form a miniature controlled atmosphere environment, so that the respiratory rate of navel oranges can be reduced, the quality of navel oranges can be maintained, moisture loss can be reduced, the navel oranges are protected from invasion of microorganisms, drosophila and other insect pests in the environment, and the green, safe and efficient storage and preservation technology and method, which can be used for prolonging the preservation time and well keeping quality and flavor of naveloranges at normal temperature, have great practical meaning and commercial value.

The invention discloses an Agrobacterium tumefaciens-mediated genetic transformation method of Penicillium italicum and belongs to the field of genetic transformation. The method of the invention is simple to perform, high in transformation rate and good in transformation stability, provides efficient genetic transformation of Penicillium italicum successfully, makes it possible to perform homologous knockout, overexpression and heterologous expression in Penicillium italicum, and lays basis for the genetic improvement of this strain by genetic engineering means and the study on pathogenesis of citrus infection by the strain.

A phenanthroindolizidine alkaloid, and a preparing method and applications thereof are disclosed. Blackend swallowwort root which is a Chinese herbal medicine is adopted as a raw material and is extracted with a solvent; and after an extract is extracted with an n-butanol solvent, an obtained extract is separated by macroporous resin column chromatography, silica gel column chromatography, ODS-C<18> reverse silica gel column chromatography, Sephadex LH-20 gel column chromatography and preparative high performance liquid chromatography in order to obtain one high-purity phenanthroindolizidine alkaloid, with the purity of the phenanthroindolizidine alkaloid obtained being 98.5%. The alkaloid is identified as 7-demethoxytylophorine. The obtained alkaloid is subjected to bacteriostatic activity research on penicillium italicum, and the 7-demethoxytylophorine has good bacteriostatic effects on the penicillium italicum and can be developed into a novel plant-sourced citrus preservative.

The invention discloses a preparation method and application of a tupistra extract. According to the preparation method of the tupistra extract, with tupistra rhizomes as raw materials and ethanol water or ethyl acetate as a solvent, extraction is conducted, then through concentration and drying, the tupistra extract is obtained, and when the solvent is ethanol water, the volume fraction of ethylalcohol is not smaller than 60%. According to the preparation method, ethanol water or ethyl acetate is used as the solvent for preparing the tupistra extract, one-step completion is achieved, multiple solvents do not need to be adopted for repeated extraction, the technical operation is simple, the extraction rate is high, the cost is low, and the method is environmentally friendly; through the prepared exact, growth of filamentous fungi can be effectively inhibited, particularly, the inhibition function on rotten fungi such as penicillium expansum, penicillium italicum wehmer, penicillium digitatum, aspergillus niger, rhizopus stolonifera and botrytis cinereal is obvious, the effect is good at low concentration (1mg/mL), the antimicrobial spectrum is wide, no harmful organic solvent residues exist, the method is environmentally friendly, the toxic or side effect on a non-target is small, and the method accords with an environment-friendly sustainable development idea.

The invention provides a method for inhibiting the growth of penicillium and reducing the postharvest rotting rate of citrus fruits, and belongs to the technical field of postharvest storage and processing of fruits. According to the method for inhibiting the growth of the penicillium italicum, the natural plant materials release gaseous substances for inhibiting the growth of the penicillium italicum, so that the effect of reducing the rotting rate of picked citrus fruits is achieved. According to the penicillium growth inhibition method and the biological treatment mode for storing the citrus based on the penicillium growth inhibition method, decay can be effectively reduced, and the penicillium growth inhibition method and the biological treatment mode have the advantages of being safe, non-toxic, environmentally friendly, low in energy consumption, convenient to operate, capable of being implemented on a large scale and the like. On the basis of the principles of effectiveness, environmental protection, simplicity and convenience, the method capable of reducing the rotting rate of the picked citrus is provided, the current situation that fruits are rotted and deteriorated in the storage and transportation process after commercial maturity and much loss is caused can be effectively improved, and the trade competitiveness of the citrus industry in China is improved.