Application of camellia oleifera extract in antisepsis and preservation

A technology of extract and Camellia oleifera is applied in the new application field of Camellia oleifera extract in fruit preservation and preservation, and achieves the effects of significant social and economic benefits, good control effect and good preservation activity.

Inactive Publication Date: 2011-12-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But there is no literature report about whether Camellia oleifera extract has fruit antiseptic and fresh-keeping activity

Method used

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  • Application of camellia oleifera extract in antisepsis and preservation
  • Application of camellia oleifera extract in antisepsis and preservation
  • Application of camellia oleifera extract in antisepsis and preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of camellia oleifera methanol extract

[0019] Weigh 20g of the dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 60h, and then extract with ultrasonic vibration for 1h. Continuously extract 4 times according to the above method, combine the 4 extracts, and concentrate the extract under reduced pressure below 50°C.

[0020] Weigh 20g of camellia oleifera plant material dry powder into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 72h, and then extract by ultrasonic vibration for 0.5h. Continuously extract 2 times according to the above method, combine the 2 extracts, and concentrate the extract under reduced pressure below 50°C.

[0021] Weigh 20g of dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature...

Embodiment 2

[0022] Example 2: Indoor Toxicity Determination of Camellia oleifera Methanol Extract to 10 Kinds of Fruit Postharvest Pathogenic Fungi

[0023] Bioassay

[0024] Any one of the extracts described in Example 1 was dispersed and dissolved to different volumes with 90% methanol to prepare active test solutions with different concentrations to be tested. Under sterile conditions, prepare different concentrations of active test solutions to be tested, then pour them into the melted culture medium (temperature is about 60°C), shake well, and pour them into a 90cm petri dish while hot to make a drug-containing culture medium. base plate. After the plate is solidified and cooled, use an inoculation needle to inoculate various phytopathogenic fungal cakes (Φ=5.0mm) with the same size and growth. One cake is connected to each plate, and the side with colonies faces down, and 3 cakes are used for each treatment. Repeat, and set water blank control and 1% methanol control at the same t...

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Abstract

The invention discloses an application of camellia oleifera extract in antiseptic and fresh preservation. The camellia oleifera extract can be applied to post-harvest fruit freshness preservation, and the post-harvest pathogens to fruit include Penicillium italicum Wehmer, Penicillium digitatum Sacc., Geotrichum candidum Link ), banana crown rot (Colletotrichum musae spp), banana anthracnose (Colletorchum musae), litchi downy mildew (Peronophythora litchi), litchi anthracnose (Colletotrichum gloeosporioides Penz.), loquat anthracnose (Colletotrichum acutatum Simmonds), loquat Alternaria Tenuis Nees, Pestalotiopsis eriobotryfolia, etc. have good control effects, and have good fresh-keeping activities on various fruits such as citrus, litchi, banana, loquat, longan, and mango. According to this characteristic of camellia oleifera, an efficient and pollution-free green plant-source fruit preservative can be developed, which has significant social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of production of anti-corrosion and fresh-keeping agents, and in particular relates to a new application of camellia oleifera extract in anti-corrosion and fresh-keeping of fruits. Background technique [0002] With the continuous improvement of people's living standards and health awareness, people gradually pay attention to the use of fruit preservatives while eating fruits. However, there are very few commercialized products of botanical fruit preservatives, and the research on botanical fruit preservatives is very urgent and has greater potential. [0003] Camellia oleifera (Camellia oleifera Abel) is a unique woody tree species in my country. It is distributed in more than 400 counties in 15 provinces including Hunan, Jiangxi, Guangxi and Fujian, with a total area of ​​more than 4 million square hectares and a cultivation history of more than 2,000 years (Cheng Jianfeng , Pan Xiaoyun, 1998), has the cha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/153
Inventor 钟国华胡美英丘麒郝卫宁翁群芳胡黎明
Owner SOUTH CHINA AGRI UNIV
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