Application of sasanqua extract in antisepsis and preservation
A technology of extract and Camellia oleifera, which is applied in the new application field of camellia oleifera extract in fruit preservation and preservation, achieving significant social and economic benefits, good control effect, and good fresh-keeping activity
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Embodiment 1
[0018] Embodiment 1: the preparation of camellia oleifera methanol extract
[0019] Weigh 20g of the dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 60h, and then extract with ultrasonic vibration for 1h. Continuously extract 4 times according to the above method, combine the 4 extracts, and concentrate the extract under reduced pressure below 50°C.
[0020] Weigh 20g of camellia oleifera plant material dry powder into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 72h, and then extract by ultrasonic vibration for 0.5h. Continuously extract 2 times according to the above method, combine the 2 extracts, and concentrate the extract under reduced pressure below 50°C.
[0021] Weigh 20g of dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature...
Embodiment 2
[0022] Example 2: Indoor Toxicity Determination Bioassay Method of Methanol Extract of Camellia oleifera to 10 Kinds of Fruit Postharvest Pathogenic Fungi
[0023] Any one of the extracts described in Example 1 was dispersed and dissolved to different volumes with 90% methanol to prepare active test solutions with different concentrations to be tested. Under sterile conditions, prepare different concentrations of active test solutions to be tested, then pour them into the melted culture medium (temperature is about 60°C), shake well, and pour them into a 90cm petri dish while hot to make a drug-containing culture medium. base plate. After the plate is solidified and cooled, use an inoculation needle to inoculate various phytopathogenic fungal cakes (Φ=5.0mm) with the same size and growth. One cake is connected to each plate, and the side with colonies faces down, and 3 cakes are used for each treatment. Repeat, and set water blank control and 1% methanol control at the same t...
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