80 results about "Penicillium cainii" patented technology

The invention discloses paecilomyces lilacinus and a cultivating method thereof and applications thereof. The method comprises steps of: A. activation of paecilomyces lilacinus PL461, which comprises steps of: placing paecilomyces lilacinus PL461 bacterial strains on a PDA medium board, cultivating the paecilomyces lilacinus PL461 bacterial strains, and activating the paecilomyces lilacinus PL461 bacterial strains; B. liquid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: putting the activated paecilomyces lilacinus PL461 bacterial strains into an Erlenmeyer flask containing a liquid medium of PDB and Czapek's, cultivating the above bacterial strains with shaking, and taking the above cultivated bacterial strains as standby; C. solid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: mixing a solid fermentation cultivation medium uniformly according to a volume ratio, wherein the medium comprises dry compost rice bran, corn flour and water, putting the mixed medium into a domestic fungus culture bag, inoculating the paecilomyces lilacinus PL461 fermented bacteria solution into the solid fermentation cultivation medium employing culture dish fermentation after disinfection and cooling, and putting the above inoculated solid fermentation cultivation medium into a culture dish after stirring uniformly again to cultivate and take as standby; and D. preparation of a paecilomyces lilacinus bacteria agent from the solid phase culture according to a formula and the method. The paecilomyces lilacinus has an obvious lethal effect on nematode eggs, equipment is simple, production cost is reduced greatly, and the cultivating method is suitable for mass production of paecilomyces lilacinus.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol A1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. Experiments prove that the compound has better anti-tumor activity on various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal squamous carcinoma cells KYSE450, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC and human gastric carcinoma cells HGC-27.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol B1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. The compound has good anti-tumor activity to various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells SGC-7901, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC, human gastric carcinoma cells HGC-27 and human lymphoma cells RAJI.

The invention discloses a celluase producing bacterium. According to the invention, the classification name of the celluase producing bacterium is Penicillium piceum, the 18S rDNA (ribosomal deoxyribonucleic acid) gene sequence of a strain is shown in SEQ ID NO1, and the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preserving number of CGMCC5314 and the preserving date of October 9, 2011, Institute Microbiological Chinese Academy of Sciences, No.3 Beichen West Road 1 Chaoyang district, Beijing city. The celluase producing bacterium disclosed by the invention has good celluase and hemicellulase producing capabilities, and especially has good Beta-glucosidase production capacity. The celluase producing bacterium disclosed by the invention and the produced celluase and hemicellulase have wide application prospects in multiple industries such as biological fuel, medicine, daily chemical industry and food fermentation.

The invention discloses a method for preparing nuclease P1 through penicillium citrinum semi-continuous fermentation. A fermentation culture medium containing vitamin B substances is used for preparing the nuclease P1 through the penicillium citrinum semi-continuous fermentation. According to the method disclosed by the invention, the single batch fermentation time is shortened from 60 hours to 20 hours, the enzyme activity is improved from 3000 U / mL to 6500 U / mL, and more than 12 batches can be continuously fermented. With the adoption of the method, the fermentation time can be obviously shortened, and the production cost can be reduced.

The invention discloses a novel chitinase gene of nematode bio-control fungi lilac paecilomyces with a gene sequence table as seen in SEQ ID NO: 1, and meanwhile discloses a method for cloning the chitinase gene of the lilac paecilomyces. The method comprises the steps of using lilac paecilomyces RNA with highest enzyme activity as a template, designing and expanding a pair of degenerate primers to get the chitinase gene fragments of the lilac paecilomyces, obtaining gene 5' terminal and 3' terminal sequences from a lilac paecilomyces cDNA chain through SMART RACE RT-PCR technology, and connecting the three sequences to obtain a novel cDNA full-length sequence of the chitinase gene (PL Chil). The invention provides important technological base for transforming the chitinase gene of the lilac paecilomyces to other nematode bio-control fungi by using bioengineering technology, realizing the heterologous expression of the gene, and improving the expression quantity of the chitinase gene of the nematode bio-control fungi and the bio-control ability of plant nematodes.

The invention discloses a marine fungi penicillium crustosum bacterial strain, quinolinone compounds derived from marine fungi penicillium crustosum, and a preparation method and applications of the quinolinone compounds. Specific structural formulas of the quinolinone compounds are represented by formula R1-formula R6. According to the preparation method, a culture medium is inoculated with the marine fungi penicillium crustosum bacterial strain AP2T1 CGMCC No.8516 for static fermentation; an obtained fermentation broth is extracted with ethyl acetate, and mycelium is extracted with an organic solvent; obtained crude extracts are mixed, an obtained mixture is subjected to sephadex column chromatography, reverse-phase silica-gel column chromatography, and thin layer chromatography detection using organic solvents; an obtained elution ingredient containing intermediates is subjected to methylation, and thin layer chromatography and reverse-phase silica-gel column chromatography separation and purification so as to obtain target compounds R1-R6. The target compounds R1-R6 possesses wide application prospect in preparation of antitumor drugs, antibacterial agents, and agricultural insecticides.

The invention discloses a compound microbial agent of nematode egg parasitical fungi and a spore germination accelerating agent, and a preparation method and application of the compound microbial agent. The compound microbial agent of the nematode egg parasitical fungi and the spore germination accelerating agent comprises paecilomyces lilacinus spore powder or fermentation broth and the spore germination accelerating agent. The best combination with various nutrient substances, which can be used for promoting the germination of paecilomyces lilacinus spores, is obtained through orthogonal experiments, and the bio-control effect of the compound microbial agent for promoting the germination of the paecilomyces lilacinus spores and controlling root-knot nematodes is verified through indoor bioassay experiments. After the compound microbial agent acts on the root-knot nematodes for five days, the egg parasitism rate of the root-knot nematodes can reach more than 80 percent, and the egg hatchability of the root-knot nematodes is almost zero, which are obviously superior to those of a control group. Experimental results prove that the spore germination accelerating agent added in the compound microbial agent can be used for effectively promoting spore germination, so that the parasitism rate of paecilomyces lilacinus to root-knot nematode eggs is improved, the acting time is shorted, and finally the control effect on the root-knot nematodes of plants is significantly improved.

The invention discloses a Paecilomyces thermophila mutant strain and a mutation induction method and application thereof. The mutation induction method includes: Paecilomyces thermophila screened from high-temperature-fermented compost and used for producing high-temperature-resistant and acid-resistant glucose oxidase is used as the original strain, protoplast ultraviolet and ethyl methane sulfonate compound mutation induction is performed, and the screened strain is subjected to budding spore diethyl sulfate-microwave compound mutation induction to obtain the Paecilomyces thermophila mutant strain. The Paecilomyces thermophila mutant strain is preserved in China General Microbiological Culture Collection Center on January 11th, 2016, and the preservation number of the mutant strain is CGMCC No. 13182. Compared with a conventional mutation induction method, the mutation induction method has the advantages that the method is high in efficiency and simple in fast in screening, the productivity of the mutant strain is high, the yield of the mutant stain is 277.65% of that of the Paecilomyces thermophila original strain, fermentation liquor enzyme activity reaches 185U / ml, production cost is lowered, and the glucose oxidase produced by the mutant strain is good in high-temperature resistance and acid resistance and can be used as feed enzyme applied to feed industry.

The invention provides a paecilomyces lilacinus fermentation production method and a paecilomyces lilacinus secondary fluid medium. Paecilomyces lilacinus strain is activated, and the nitrogen content in a primary fluid medium is high to promote the paecilomyces lilacinus strain to focus on thallus production, so that more robust thalli are obtained. In the secondary seed solution medium, the robust thalli can generate more spores, paecilomyces lilacinus spores can germinate in the secondary fluid medium, so that a large number of thalli can be generated quickly in solid culture, and the number of generated thalli is 3.0*1010 cfu / g or above.

The invention relates to a method for producing fermentative cordycep fungal powder by solid fermentation of mixed strains. The method comprises the following steps: carrying out slant culture, producing liquid strains, carrying out solid fermentation, and drying and grinding. The method is characterized in that the fermented strains are hirsutella sinensis, paecilomyces hepialid and cephalosporins. According to the method, in combination with the fermentation and growth characteristics of the hirsutella sinensis, the paecilomyces hepialid and the cephalosporins, solid fermentation is carried out on the three strains to prepare the fermentative cordycep fungal powder having ingredients closer to natural cordyceps sinensis and having more perfect effects; therefore, good economic benefits are brought to artificial cordyceps sinensis industrialization.

Aiming at the drawbacks of the prior art, the invention applies for providing a method for breeding a penicillium gene engineering bacterium having high lipase synthesis capability. The method comprises: obtaining a hygromycin-resistance expression box and cloning to a target plasmid to obtain a hygromycin-resistance plasmid; amplifying the lipase gene, namely primary effusion lymphoma (PEL), of the penicillium and the strong promoter, namely glyceraldehyde-3-phosphate dehydrogenase promoter PgpdA, of Aspergillus nidulans and tryptophan synthetase terminator TtrpC of the Aspergillus nidulans respectively to obtain a PEL gene expression box driven by the strong promoter; inserting the PEL gene expression box into the hygromycin-resistance plasmid to obtain a PEL gene super expression vector containing a hygromycin screening marker; and finally, transferring Agrobacterium rhizogenes by using the PEL gene super expression vector containing the hygromycin screening marker, and transferring the PEL gene expression box into a penicillium strain by using a Agrobacterium rhizogenes-mediated transfer method to obtain the penicillium gene engineering bacterium with high lipase expressing capability.

The invention discloses a Paecilonyces variotii bacterial strain having high virulence to citrus psylla and an application thereof and relates to the technical field of biological prevention and control. The bacterial strain is Paecilonyces variotii GZMS-11, preserved in China Center for Type Culture Collection on October 29, 2015 with the preservation number CCTCC NO: 2015651. The Paecilonyces variotii GZMS-11 has high virulence to citrus psylla, can be developed into biopesticide for biological prevention and control of citrus psylla. Compared with existing chemicals for prevention and control of citrus psylla, the Paecilonyces variotii GZMS-11 has the advantages of being highly efficient, environment-friendly, high in persistence and not easy to cause drug resistance, and has no pollution to the environment and results in no residue. The bacterial strain of the Paecilonyces variotii GZMS-11 grows fast, has high sporulation yield and high germination rate of spores, is easy to prepare, and has good market perspective.

The invention discloses penicillium purpurescens QL-9204 and application for preparing phloretin during phlorizin conversion. The penicillium purpurescens QL-9204 is a strain free of toxicity and harm and safe to use. The penicillium purpurescens QL-9204 grows rapidly, and is high in hybrid bacteria resistance, easy to culture and stable in batch; a fermentation medium is simple in components and low in market price, thereby being low in production cost; a substrate plorizin is directly put into a crude enzyme for conversion, the enzyme separation and purification steps are omitted, the technology is simple, and the penicillium purpurescens QL-9204 is easy to apply industrially; the phloretin conversion yield is high and can reach 92.5% to the maximum, and the penicillium purpurescens QL-9204 has the advantages that few by-products are produced, and the product is easy to extract.

The invention relates to a nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and a preparation method thereof. The nematicidal composition consists of the verticillium chlamydosporium and the Paecilomyces lilacinus, and also comprises a solvent, an aid and a stabilizer. The nematicidal composition comprises the following components in percentage by weight: 0.1 to 10 percent of verticillium chlamydosporium, 0.1 to 20 percent of Paecilomyces lilacinus, 1 to 15 percent of stabilizer, 5 to 15 percent of solvent, 1 to 10 percent of aid and 60 to 90 percent of carrier. The verticillium chlamydosporium and the Paecilomyces lilacinus are combined to achieve a synergistic effect, and a nematicidal effect is better; the verticillium chlamydosporium and the Paecilomyces lilacinus are bacterial parasites for nematode, a biocontrol technique is used, and is environment-friendly and safe, and problems that the nematode has resistance to pesticides, a development cycle for a new pesticide is long and the like are solved; and the verticillium chlamydosporium and the Paecilomyces lilacinus can promote the growth of plants to achieve effects of increasing production and value.

The invention discloses a preparation method of paecilomyces lilacinus spore powder. The method comprises the following steps of: (1) preparing a paecilomyces lilacinus liquid fermentation broth; (2) recovering conidia from the paecilomyces lilacinus liquid fermentation broth; and (3) drying the recovered paecilomyces lilacinus conidia to obtain the paecilomyces lilacinus spore powder. According to the invention, the technological parameters for preparing paecilomyces lilacinus spore powder are screened and optimized to obtain optimal technological parameters for preparation, thus the yield and product quality of paecilomyces lilacinus spores are effectively improved. The preparation method of paecilomyces lilacinus spore powder, disclosed by the invention, has the advantages of high recovery rate, high spore content, high live spore rate, low water content and the like, and conforms to the requirements of large-scale industrial production.

The invention discloses a novel Penicillium sp. HS-NF-684Z, deposit No. CGMCC No. 14144; also disclosed is a method for preparing fumiginin or a pharmaceutical composition containing fumiginin by culturing the same.

The invention discloses a Verticillium Paecilomyces varioti strain. When the strain is treated on a czapek's medium at 25 DEG C for 14 days, the bacterial colony has a diameter of 35-55 mm, is raised, is of white villus, is in an irregular shape and has a white back side. Hyphae have a width of 1.2-1.8 mu m and can form conidiophores with a length of 9.6-19.8 mu m, majority of the phialides are cylindrical or tapered and are often whorled or alternate and grow on aerial hyphae in opposite, the hyphae gradually or suddenly tapers upwards and have a thin neck of 7.8-14.4*1.2-2.4 mu m, and the length is almost 1.2 times longer than that of the base of the Verticillium Paecilomyces varioti. The conidiophores are transparent and smooth and can be distinctly divided into two spores with different sizes, wherein the macro spores are in the shape of fusiformis or ellipse and have a size of 1.8-3.0*1.8-2.4 mu m, and the micro spores are subglobose or elliptic, have a size of 1.2-1.8*0.6-1.2 mu m and can form a linear chain or cluster. The invention also discloses an application of the Verticillium Paecilomyces varioti strain. The strain can be used for producing Cordycepin by fermentation and has the advantages of easy culture, storage and industrial production.

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.

The invention discloses application of a biocontrol bacterium paecilomyces fumosoroseus strain capable of expressing green fluorescent protein (GFP) to the targeted pest epidemiology. According to the biocontrol bacterium, through protoplast transformation technology, an expression plasmid vector pCT74-GFP with green fluorescent protein marks is converted into a genome of the paecilomyces fumosoroseus strain FZ01, and the biocontrol strain FZ01-GFP capable of expressing the GFP is obtained. The bacterial strain is used for researches on the targeted pest epidemiology, the biocontrol strain FZ01-GFP is released, collection is conducted at a fixed time and location, the number of targeted pests is recorded, sick pest samples are collected, PCR molecular detection identification is conducted on the sick pest samples through a pair of specific primers of the plasmid vector pCT74-GFP, data are analyzed, and the plasmid vector pCT74-GFP and tendency of targeted pest infection of the biocontrol strain FZ01-GFP are determined; the problem that it is difficult to identify the pathogenesis of the targeted pests in the researches on the biocontrol bacterium epidemiology in the prior art is solved; the application has the advantages of being simple in step, convenient to operate and capable of effectively monitoring and objectively reflecting the targeted pest occurrence and development rules of the biocontrol bacterium paecilomyces fumosoroseus strain and the like.

The invention provides a Paecilomyces lilacinus granule. The Paecilomyces lilacinus granule comprises 5-15 parts of a medicinal active component which is Paecilomyces lilacinus, 10-25 parts of an assistant, 50-90 parts of a medicine carrier and 15-25 parts of water. The effective spore amount of the Paecilomyces lilacinus granule is 1.8-2.2 * 10<8> cfu / g, the competitor rate is not greater than 0.1%, and the shedding rate is not greater than 3%. The Paecilomyces lilacinus granule has the advantages of high efficiency, low toxicity, stable insecticidal effect, safety to people, animals and crops, and high safety. An experiment result shows that the control effect of Paecilomyces lilacinus reaches up to 78% or above.

The invention provides paecilomyces fumosoroseus oil which comprises the following components in percentage by mass: 30-50% of paecilomyces fumosoroseus, 15-40% of a solvent, 2-20% of an emulsifier, 2-40% of an adsorbent, 1-10% of an ultraviolet absorbent, 1-10% of an antioxidant and 2-20% of a dispersing agent. The paecilomyces fumosoroseus oil prepared by the invention is respectively stored in environments at the temperature of 4 DEG C and 25 DEG C, the germination rate is determined every six months, and the germination rate within 24 months is respectively 90.4% and 82.9%. The paecilomyces fumosoroseus oil prepared by the invention has an insect dropping rate of 69.1-86.0%.

The invention discloses a strain of Penicillium fungus M1 and application thereof to increase of saponins yield in a fermentation process of ginseng or American ginseng, and belongs to the technical field of biotechnology. According to the invention, functional fungus is used for fermentation cultivation of ginseng or American ginseng; the content of saponins before and after the fermentation are analyzed and compared; and finally a bacterial strain capable of significantly increasing saponins yield in the fermentation process of the two herbs is obtained. The strain is identified as Penicillium fungus (Penicillium sp.), and named after Penicillium fungus M1, and has a preservation number of CGMCC NO.6359. The strain can be used in fermentation production of ginseng or American ginseng; and the content of saponins in fermentation products fermented by the strain is greatly improved. Therefore, according to the present invention, Penicillium fungus has broad application prospects in increasing saponins yield in the fermentation process of ginseng or American ginseng.

The invention aims to provide a strain for preventing and treating myzus persicae. The preservation number of the strain is CGMCCNO. 11659. The mfn21 strain obtained by the invention has a biological activity on the myzus persicae, and can be applied to prevention and treatment of the myzus persicae on tobacco. A bioassay adopting a micro-drop method shows that the lethality of a secondary metabolite in a fermentation broth to the myzus persicae can reach up to 97.78%. After chemical analysis and structural identification by adopting GC / MS is performed, the secondary metabolite comprises seven main compounds including two alkaloid compounds, two ester compounds, a glycoside compound, a peptide compound and an alcohol compound.

The invention belongs to the technical filed of microbes, and concretely relates to Penicillium ochrochloron and its application in the preparation of brefeldin A. A technical problem to be solved in the invention is providing a new choice for production of BFA through biological fermentation. A bacterial strain produced in the invention is Penicillium ochrochloron SWUKD4.211, and has a preservation number of CCTCC M2015022. The invention also provides a method for producing the BFA by adopting Penicillium ochrochloron fermentation. The BFA production ability of the bacterial strain obtained through the method can reach 356.0mg / L, and the method provides a new choice for the production of the BFA, provides a precisou strain for biological pharmacy, and has great application values.

The invention belongs to the field of biotechnology, and relates to a penicillium expansum strain for degrading liquor distillers' grain cellulose. The penicillium expansum strain for degrading liquor distillers' grain cellulose is named penicillium expansum N53. The penicillium expansum N53 provided by the invention can degrade cellulose in liquor distillers' grains into glucose, has the characteristics of high cellulase activity, high conversion rate from cellulose into glucose and the like, greatly improves the utilization rate of the liquor distillers' grains, provides guarantee to follow-up generation of protein feed, and is a liquor distillers' grain cellulose degrading strain with great values in research and development. Few researches are available about fermenting and degrading the liquor distillers' grain cellulose by use of penicillium expansum.

The invention relates to the technical field of molecular biology, and discloses a method for identifying a paecilomyces cicadae strain CGMCC No. 3453. The method comprises the following steps of: extracting DNA (Deoxyribonucleic Acid) of the strain to be detected; performing PCR (Polymerase Chain Reaction) amplification on the DNA of the strain to be detected by using primers S6, S10, S31, OPV-14 and OPW-06 respectively; performing agarose gel electrophoresis on the obtained amplification products respectively to obtain an RAPD (Random Amplified Polymorphic DNA) band graph of the strain to be detected; and comparing the obtained RAPD band graph of the strain to be detected with the RAPD band graph of a standard sample and judging to obtain an identification result. By using the identification method provided by the invention, the paecilomyces cicadae strain CGMCC No. 3453 can be quickly and accurately identified without being affected by environment.

The invention belongs to the field of microbial biotechnology, and relates to a strain of Penicillium dermatophilum (P. crustosum) Pc-46 for producing Lovastatin by liquid fermentation. Specifically,the invention discloses a Pc-46 Penicillium crustosum Pc-46 (deposit No.: CCTCC NO: M 2018343), and also discloses an application of the Penicillium dermatophilum use and fermentation production method. Specifically, the present invention discloses Penicillium dermatophilum (P. crustosum)Pc-46 for preparing lovastatin; and the yield of Lovastatin is about 155mg / L to 158mg / L.

The invention provides a liquid fermentation method of paecilomyces lilacinus. The method comprises the steps of activation of culture, expanding cultivation of culture and fermentation. According to the fermentation step, an adopted fermentation medium comprises cottonseed cake powder, kieselguhr, yeast extract powder, sweet potato powder, white granulated sugar, fish peptone, olive oil, mannitol, magnesium sulfate, copper sulfate, polysorbate 20, calcium hydrophosphate, boric acid, bile salt, tyrosine, manganese dioxide and deionized water. According to the step of activation of culture, an adopted medium for activation of culture comprises algal polysaccharides, sorbitol, beef extract, asparagine, brochantite, manganese dioxide, sodium chloride, calcium chloride, boric acid, ferric citrate, mannitol, agar and deionized water. According to obtained paecilomyces lilacinus fermentation liquid, the content of spores is 4.1*10<9>-4.4*10<9> / g.