Method for producing fermentative cordycep fungal powder by solid fermentation of mixed strains
A technology of Cordyceps sinensis powder and solid fermentation, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc. It can solve the problems of limited efficacy and composition that is difficult to approach natural Cordyceps sinensis, and achieve good results. The effect of economic benefits
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Embodiment 1
[0023] Take the three strains purchased or isolated, Paecilomyces chinensis, Paecilomyces spp. Bacteria were cultured at room temperature for 8 days, and the composition of the slant medium was calculated by weight volume ratio: broad bean powder 5%, glucose 2%, corn flour 3%, peptone 0.75%, agar 2%, bran 1.5%, magnesium sulfate 1%, Dipotassium hydrogen phosphate 0.2% and water 84.55%.
[0024] The completed culture of Mortierella sinensis is inoculated on the expanded production medium, the inoculation amount is 5-10%, and the first expanded production is carried out at 15° C., and the time is 7 days; the composition of the expanded production medium is calculated as follows: : Glucose 2%, yeast extract 0.5%, peptone 1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.8%, water 95.6%.
[0025] Mix the Mortierella chinensis after the first expanded production with the completed culture of Paecilomyces hematalis and Cephalosporium sinensis, and inoculate them together...
Embodiment 2
[0029] Take the three strains purchased or isolated, Paecilomyces chinensis, Paecilomyces spp. Bacteria were cultured at room temperature for 10 days, and the composition of the slant medium was calculated by weight volume ratio: broad bean powder 3%, glucose 4%, corn flour 1%, peptone 0.5%, agar 1%, bran 4%, magnesium sulfate 0.1%, Dipotassium hydrogen phosphate 0.05%, water 86.35%.
[0030] The completed culture of Mortierella sinensis is inoculated on the expanded production medium, the inoculation amount is 5-10%, and the first expanded production is carried out at 18° C., and the time is 7 days; the composition of the expanded production medium is calculated by weight volume ratio: : Glucose 3%, yeast extract 4%, peptone 0.5%, magnesium sulfate 1%, dipotassium hydrogen phosphate 1%, water 90.5%.
[0031] Mix the Mortierella chinensis after the first expansion of production with the completed culture of Paecilomyces hematalis and Cephalosporium sinensis, and inoculate the...
Embodiment 3
[0035] Take the three strains purchased or isolated, Paecilomyces chinensis, Paecilomyces spp. Bacteria were cultured at room temperature for 9 days, and the composition of the slant medium was calculated by weight volume ratio: broad bean powder 4%, glucose 2.5%, corn flour 4%, peptone 2%, agar 0.5%, bran 1%, magnesium sulfate 0.8%, Dipotassium hydrogen phosphate 1%, water 84.2%.
[0036] The completed culture of Mortierella sinensis is inoculated on the expanded production medium, the inoculation amount is 5-10%, and the first expanded production is carried out at 16° C., and the time is 8 days; the composition of the expanded production medium is calculated as follows by weight volume ratio : Broad bean powder 4%, glucose 5%, corn flour 3%, peptone 1%, yeast extract 2%, magnesium sulfate 0.9%, dipotassium hydrogen phosphate 0.2%, water 83.9%.
[0037] Mix the Mortierella chinensis after the first expansion of production with the completed culture of Paecilomyces hematalis ...
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