Penicillium expansum strain for degrading liquor distillers' grain cellulose

A technology of distiller's grains and Penicillium extensa, which is applied in the biological field and can solve the problems of poor cellulose degradation ability, unstable enzyme activity, and high cost of enzyme production

Active Publication Date: 2014-03-26
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these cellulolytic strains that have been researched or applied still have problems such as unstable enzyme activity, poor ce...

Method used

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  • Penicillium expansum strain for degrading liquor distillers' grain cellulose
  • Penicillium expansum strain for degrading liquor distillers' grain cellulose
  • Penicillium expansum strain for degrading liquor distillers' grain cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Screening of Penicillium extensa strains for degrading distiller's grain cellulose

[0070]After the microorganisms in the distiller's grains and soil samples are enriched and cultivated, they are gradually diluted to an appropriate concentration and applied to the screening medium, cultivated at 30°C for 3-4 days, and then pick a single colony and transfer them to the slant medium for 30°C. -4 days, then streaked into the screening medium and cultivated at 30°C. After the growth of a single colony, add its mass fraction of 1% Congo red solution for staining for 2 hours, and then decolorize it with a mass fraction of 1% NaCl solution for 1 hour, and observe whether there is any The generation of the transparent circle, and record the size of the diameter of the transparent circle and the diameter of the colony, such as figure 1 . Select the strain with a larger ratio of the diameter of the transparent circle to the diameter of the colony for re-screening. In...

Embodiment 2

[0079] Example 2 Penicillium expansum CGMCC NO.7275 18S rDNA sequencing

[0080] The distiller's grain cellulose-degrading strain N53 obtained in Example 1, that is, the CGMCC No. 7275 strain, was entrusted to Jinan Lige Technology Co., Ltd. to perform 18S rDNA sequencing.

[0081] The experimental method is: pick the slant culture and put it in 700 μl 65°C preheated FPCB Solution and 7 μl β-mercaptoethanol, centrifuge in a water bath at 65°C to take the supernatant as a template, use UNIQ-10 Genomic DNA Preps Kit, and use NS1 and NS6 as templates. Primers to amplify the target fragment. Take 5 μl for agarose gel electrophoresis, use UNIQ-10 Spin Column DNA Gel Extraction Kit to cut the gel and recover the target fragment, and use Seq Forward and Seq Reverse Seq Internal as primers to perform DNA sequencing on the recovered product.

[0082] Sequencing results: the nucleotide sequence of Penicillium expansum (Penicillium expansum) CGMCC NO.7275 18S rDNA sequencing is shown in...

Embodiment 3

[0126] Example 3 Application of Penicillium expansum CGMCC NO.7275 Strain

[0127] Transplant the Penicillium expansum CGMCC NO.7275 strain to the slant for activation;

[0128] Configure the slant medium according to the composition of the above slant medium, and after streaking at 30°C for 3-4 days, pour sterile water and scrape the spores to prepare the spore suspension. Liquor distiller's grains 10-90 g / L, bran 10-90 g / L (the ratio of the two can range from 1:9 to 9:1), soybean meal 5-40 g / L, (NH4)2SO4 0-20 g / L L, KH2PO4 0-4 g / L, MgSO4 7H2O 0-1.5 g / L, GaCl2 2H2O 0.05-1 g / L, MnSO4 H2O 0-0.4 g / L, ZnSO4 7H2O 0-0.2 g / L, Composition of pH 4.0-8.0 medium Prepare fermentation medium, the initial cellulose content is 248g / L (according to the previous content of bran and distiller's grains, change appropriately), the liquid volume in a 250ml triangular bottle is 30ml, steam sterilization at 121°C for 20 minutes , cooled to room temperature, inoculated with 1ml of spore suspension...

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Abstract

The invention belongs to the field of biotechnology, and relates to a penicillium expansum strain for degrading liquor distillers' grain cellulose. The penicillium expansum strain for degrading liquor distillers' grain cellulose is named penicillium expansum N53. The penicillium expansum N53 provided by the invention can degrade cellulose in liquor distillers' grains into glucose, has the characteristics of high cellulase activity, high conversion rate from cellulose into glucose and the like, greatly improves the utilization rate of the liquor distillers' grains, provides guarantee to follow-up generation of protein feed, and is a liquor distillers' grain cellulose degrading strain with great values in research and development. Few researches are available about fermenting and degrading the liquor distillers' grain cellulose by use of penicillium expansum.

Description

(1) Technical field [0001] The invention belongs to the field of biotechnology, and relates to a Penicillium expansum strain for degrading distiller's grain cellulose. (2) Background technology [0002] Liquor distiller's grains are by-products of liquor production. Because distiller's grains are acidic and contain incompletely utilized nutrients such as starch, protein, various amino acids, vitamins, and mineral elements, they are good substrates for the growth of microorganisms. Serious pollution is not in line with my country's development policy of vigorously building a resource-saving and environment-friendly society. In recent years, the liquor industry has been developing day by day. From January to November 2012 alone, the national liquor production reached 10.2332 million kiloliters, an increase of 1.1202 million kiloliters over the same period in 2011. Therefore, the comprehensive utilization of distiller's grains is of great significance to the construction o...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12P19/14C12P19/02C12R1/83
Inventor 徐慧刘建军李文婧牛梅丽
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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