Paecilomyces lilacinus fermentation production method and paecilomyces lilacinus secondary fluid medium
A technology of Paecilomyces lilacinus and liquid culture medium, which is applied in the field of Paecilomyces lilacinus fermentation production method and the field of Paecilomyces lilacinus secondary liquid culture medium, and can solve the problems of high cost, low yield, long fermentation time, etc. question
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Embodiment 1
[0065] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strain is inoculated in the Paecilomyces lilacinosa primary liquid culture medium according to the inoculation amount of 1%, and the primary liquid medium comprises the components of the following mass content: glucose 40g / L, soybean powder 10g / L , sodium nitrate 10g / L, dipotassium hydrogen phosphate 2.06g / L, potassium chloride 0.5g / L, magnesium sulfate 0.24g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 6.8. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carri...
Embodiment 2
[0070] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 10 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strain is inoculated in the Paecilomyces lilacinus primary liquid culture medium according to the inoculum size of 0.5%, and the primary liquid medium comprises the components of the following mass content: glucose 30g / L, soybean powder 15g / L , sodium nitrate 15g / L, dipotassium hydrogen phosphate 2.2g / L, potassium chloride 0.25g / L, magnesium sulfate 0.18g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 7.0. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carried o...
Embodiment 3
[0075] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strains are inoculated in the Paecilomyces lilacinus primary liquid culture medium according to the inoculum size of 1.5%, and the primary liquid medium comprises the components of the following mass content: glucose 50g / L, soybean powder 15g / L , sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.8g / L, potassium chloride 0.75g / L, magnesium sulfate 0.18g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 6.5. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carried ...
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