Paecilomyces lilacinus fermentation production method and paecilomyces lilacinus secondary fluid medium

Abstract

The invention provides a paecilomyces lilacinus fermentation production method and a paecilomyces lilacinus secondary fluid medium. Paecilomyces lilacinus strain is activated, and the nitrogen content in a primary fluid medium is high to promote the paecilomyces lilacinus strain to focus on thallus production, so that more robust thalli are obtained. In the secondary seed solution medium, the robust thalli can generate more spores, paecilomyces lilacinus spores can germinate in the secondary fluid medium, so that a large number of thalli can be generated quickly in solid culture, and the number of generated thalli is 3.0*1010 cfu / g or above.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Paecilomyces lilacinus fermentation production method and a secondary liquid culture medium of Paecilomyces lilacinus. Background technique [0002] Nematodes can invade and parasitize plants. The normal growth and development of infected and parasitic plants are affected by the absorption of nutrients in the body by nematodes, and the secretions of nematode metabolism can stimulate the cells and tissues of host plants, resulting in plant deformities. Most plant nematodes attack the underground parts of plants, such as roots, tubers, etc., and the roots can be manifested as nodules, necrosis, stubby roots, and clusters, resulting in a large reduction in yield or quality of crops. Common plant nematode diseases include root-knot nematode disease, soybean cyst nematode disease, wheat grain nematode disease, sweet potato stem nematode disease, rice stem-point nematode disea...

AI Technical Summary

Problems solved by technology

For more than half a century, researchers at home and abroad have conducted extensive and in-depth research on Paecilomyces lilacinus, and achieved a series of research results in biology, ecology, pest control efficiency and field application. It shows great potential in prevention and control, and the market prospect is broad. Although the traditional fungal fermentation method can realize industrial production, there are problems such as long fermentation time, high cost, and low yield.