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Paecilomyces lilacinus fermentation production method and paecilomyces lilacinus secondary fluid medium

A technology of Paecilomyces lilacinus and liquid culture medium, which is applied in the field of Paecilomyces lilacinus fermentation production method and the field of Paecilomyces lilacinus secondary liquid culture medium, and can solve the problems of high cost, low yield, long fermentation time, etc. question

Inactive Publication Date: 2016-12-21
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For more than half a century, researchers at home and abroad have conducted extensive and in-depth research on Paecilomyces lilacinus, and achieved a series of research results in biology, ecology, pest control efficiency and field application. It shows great potential in prevention and control, and the market prospect is broad. Although the traditional fungal fermentation method can realize industrial production, there are problems such as long fermentation time, high cost, and low yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strain is inoculated in the Paecilomyces lilacinosa primary liquid culture medium according to the inoculation amount of 1%, and the primary liquid medium comprises the components of the following mass content: glucose 40g / L, soybean powder 10g / L , sodium nitrate 10g / L, dipotassium hydrogen phosphate 2.06g / L, potassium chloride 0.5g / L, magnesium sulfate 0.24g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 6.8. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carri...

Embodiment 2

[0070] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 10 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strain is inoculated in the Paecilomyces lilacinus primary liquid culture medium according to the inoculum size of 0.5%, and the primary liquid medium comprises the components of the following mass content: glucose 30g / L, soybean powder 15g / L , sodium nitrate 15g / L, dipotassium hydrogen phosphate 2.2g / L, potassium chloride 0.25g / L, magnesium sulfate 0.18g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 7.0. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carried o...

Embodiment 3

[0075] Preparation of the primary seed liquid of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; after activation The Paecilomyces lilacinus strains are inoculated in the Paecilomyces lilacinus primary liquid culture medium according to the inoculum size of 1.5%, and the primary liquid medium comprises the components of the following mass content: glucose 50g / L, soybean powder 15g / L , sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.8g / L, potassium chloride 0.75g / L, magnesium sulfate 0.18g / L, iron sulfate 0.05g / L, and the balance is water. The temperature of the primary liquid culture of Paecilomyces lilacinus is 28°C, and the initial pH value is 6.5. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The air flow of / h was carried ...

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PUM

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Abstract

The invention provides a paecilomyces lilacinus fermentation production method and a paecilomyces lilacinus secondary fluid medium. Paecilomyces lilacinus strain is activated, and the nitrogen content in a primary fluid medium is high to promote the paecilomyces lilacinus strain to focus on thallus production, so that more robust thalli are obtained. In the secondary seed solution medium, the robust thalli can generate more spores, paecilomyces lilacinus spores can germinate in the secondary fluid medium, so that a large number of thalli can be generated quickly in solid culture, and the number of generated thalli is 3.0*1010 cfu / g or above.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Paecilomyces lilacinus fermentation production method and a secondary liquid culture medium of Paecilomyces lilacinus. Background technique [0002] Nematodes can invade and parasitize plants. The normal growth and development of infected and parasitic plants are affected by the absorption of nutrients in the body by nematodes, and the secretions of nematode metabolism can stimulate the cells and tissues of host plants, resulting in plant deformities. Most plant nematodes attack the underground parts of plants, such as roots, tubers, etc., and the roots can be manifested as nodules, necrosis, stubby roots, and clusters, resulting in a large reduction in yield or quality of crops. Common plant nematode diseases include root-knot nematode disease, soybean cyst nematode disease, wheat grain nematode disease, sweet potato stem nematode disease, rice stem-point nematode disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14
Inventor 梁小文王根豪严艺波李肖宇吴俊杰石峥曾升华李英武
Owner JIANGXI TIANREN ECOLOGY
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