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Compound preparation of nematode egg parasitical fungi and spore germination accelerating agent and application thereof

A technology of spore germination and promoter, applied in the field of biological pesticide formulations, can solve the problems of poor parasitic effect of root-knot nematode eggs, and achieve the effects of reducing egg hatching and improving control effect.

Active Publication Date: 2013-03-27
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although Paecilomyces lilacinus has a high parasitic effect on root-knot nematode eggs and has many practical applications, it has a poor parasitic effect on the late stage of root-knot nematode eggs and second-instar larvae, and studies have found that its metabolites can also help Hatching of root-knot nematode eggs

Method used

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  • Compound preparation of nematode egg parasitical fungi and spore germination accelerating agent and application thereof
  • Compound preparation of nematode egg parasitical fungi and spore germination accelerating agent and application thereof
  • Compound preparation of nematode egg parasitical fungi and spore germination accelerating agent and application thereof

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preparation example Construction

[0036] Preparation of Preparatory Example Paecilomyces lilacinus spore powder

[0037] One, the preparation of Paecilomyces lilacinus (P.lilacinus) fermented liquid

[0038] 1. After activating the Paecilomyces lilacinus species, carry out shake flask seed culture;

[0039] The composition of shake flask seed culture medium (by mass percentage):

[0040] Glucose 1.5-2%

[0041] Sodium nitrate 0.4-0.6%

[0042] Ammonium sulfate 0.2-0.3%

[0043] Soybean powder 0.2-0.3%

[0044] Dipotassium hydrogen phosphate 0.1-0.2%

[0045] Magnesium sulfate 0.1-0.2%

[0046] water balance

[0047] Liquid fermentation conditions: put 200ml of seed medium into a 500ml triangular flask, inoculate Paecilomyces lilacinus spore suspension after autoclaving, the inoculation amount is 1.5-3%, place at 24-28°C, shaker 150-200r / min, cultivated for 18-36h.

[0048] 2. Secondary liquid expansion culture (by mass percentage):

[0049] Glucose 3-5%

[0050] Sodium nitrate 0.4-0.6%

[0051] Am...

experiment example 1

[0071] Experimental Example 1 Screening experiment of the component types and dosages of the biopesticide compound agent for controlling root-knot nematodes

[0072] 1. Materials and methods

[0073] 1.1 Tested strains and root-knot nematode eggs

[0074] Tested strains: Paecilomyces lilacinus strains were purchased from China Forestry Microorganism Culture Collection Management Center, and the commercial purchase number of the strains is: cfcc 86080;

[0075] Root-knot nematode eggs for testing: collected from tomato roots cultivated in a solar greenhouse in Zhuitun Village, suburb of Beijing, and identified as root-knot nematode incognita.

[0076] 1.2 Preparation of Paecilomyces lilacinus spore suspension

[0077] Paecilomyces lilacinus strains were inoculated on PDA plates, cultured in a constant temperature incubator at 25°C for 5-7 days, and the spores on the PDA plates were washed with 0.05% (V / V) sterile Tween-80 solution, and placed in Mix evenly on a shaker or sha...

Embodiment 1

[0110] The preparation of embodiment 1 fungus and spore germination promoter compound preparation

[0111] Weigh each component according to the following weight (unit: kg): Paecilomyces lilacinus spore powder 99.9, vitamin B10.1; mix the above components together, stir evenly, and obtain.

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Abstract

The invention discloses a compound microbial agent of nematode egg parasitical fungi and a spore germination accelerating agent, and a preparation method and application of the compound microbial agent. The compound microbial agent of the nematode egg parasitical fungi and the spore germination accelerating agent comprises paecilomyces lilacinus spore powder or fermentation broth and the spore germination accelerating agent. The best combination with various nutrient substances, which can be used for promoting the germination of paecilomyces lilacinus spores, is obtained through orthogonal experiments, and the bio-control effect of the compound microbial agent for promoting the germination of the paecilomyces lilacinus spores and controlling root-knot nematodes is verified through indoor bioassay experiments. After the compound microbial agent acts on the root-knot nematodes for five days, the egg parasitism rate of the root-knot nematodes can reach more than 80 percent, and the egg hatchability of the root-knot nematodes is almost zero, which are obviously superior to those of a control group. Experimental results prove that the spore germination accelerating agent added in the compound microbial agent can be used for effectively promoting spore germination, so that the parasitism rate of paecilomyces lilacinus to root-knot nematode eggs is improved, the acting time is shorted, and finally the control effect on the root-knot nematodes of plants is significantly improved.

Description

technical field [0001] The present invention relates to a biological pesticide preparation, in particular to a biological pesticide compound preparation of a nematode ova parasitic fungus and a spore germination accelerator. The present invention also relates to a preparation method of the biological pesticide compound The application in nematodes belongs to the field of biological control of plant parasitic nematodes. Background technique [0002] Paecilomyces lilacinus (P.lilacinus) is a resident fungus in the soil and the rhizosphere of various plants. It has a high parasitic rate to the root-knot nematode incognita, which can reach more than 80%. It has been considered that Paecilomyces lilacinus is a very important plant-parasitic nematode egg-parasitic fungus, and has been widely used in the control of harmful plant-parasitic nematodes such as root-knot nematodes and cyst nematodes. As one of the important biocontrol bacteria for the control of plant pathogenic nemato...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P5/00
Inventor 王曦茁汪来发郭志斌马建伟朴春根李永
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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