Verticillium Paecilomyces varioti strain and application thereof

A technology of Paecilomyces and verticillium, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of low content of cordycepin and difficult to meet

Inactive Publication Date: 2011-01-12
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the increasing demand for cordycepin in the market, the content of cordycepin in wild Cordyceps militaris is not high, generally 0.1% or lower, it is difficult to meet the requirement of 2% for commercial cordycepin

Method used

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  • Verticillium Paecilomyces varioti strain and application thereof
  • Verticillium Paecilomyces varioti strain and application thereof
  • Verticillium Paecilomyces varioti strain and application thereof

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Embodiment Construction

[0015] The screening method of Paecilomyces verticillium strain comprises the following steps:

[0016] (1) Isolation method of bacterial strain:

[0017] a) Preparation of soil sample bacterial solution

[0018] In the laboratory, the soil samples collected and dried under natural conditions were picked up to remove dead branches, fallen leaves, small stones and other sundries. Each soil sample was weighed 10g, dissolved in 90mL sterile water and brought with In a 250mL Erlenmeyer flask with glass beads, fully shake for 20-30 minutes to fully dissolve the soil sample and let it stand for 20 minutes to make the soil sample a soil bacterial solution

[0019] b) Preparation of soil dilution solution: use a sterile pipette to draw 1mL soil bacteria solution from the soil bacteria solution, add it into a test tube filled with 9mL sterile water and mix well, and then use a sterile pipette to remove it from the test tube Take 1mL from the solution and add it to another test tube f...

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Abstract

The invention discloses a Verticillium Paecilomyces varioti strain. When the strain is treated on a czapek's medium at 25 DEG C for 14 days, the bacterial colony has a diameter of 35-55 mm, is raised, is of white villus, is in an irregular shape and has a white back side. Hyphae have a width of 1.2-1.8 mu m and can form conidiophores with a length of 9.6-19.8 mu m, majority of the phialides are cylindrical or tapered and are often whorled or alternate and grow on aerial hyphae in opposite, the hyphae gradually or suddenly tapers upwards and have a thin neck of 7.8-14.4*1.2-2.4 mu m, and the length is almost 1.2 times longer than that of the base of the Verticillium Paecilomyces varioti. The conidiophores are transparent and smooth and can be distinctly divided into two spores with different sizes, wherein the macro spores are in the shape of fusiformis or ellipse and have a size of 1.8-3.0*1.8-2.4 mu m, and the micro spores are subglobose or elliptic, have a size of 1.2-1.8*0.6-1.2 mu m and can form a linear chain or cluster. The invention also discloses an application of the Verticillium Paecilomyces varioti strain. The strain can be used for producing Cordycepin by fermentation and has the advantages of easy culture, storage and industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Paecilomyces verticillium strains, and also relates to its use. Background technique [0002] Cordycepin (cordycepin) is a class of nucleoside antibacterial substances isolated from Cordyceps militaris for the first time. It has a variety of biological activity values. Cordycepin can be used as a substitute for Cordyceps sinensis. In 2009, it passed the certification of new food. With the increasing demand for cordycepin in the market, the content of cordycepin in wild Cordyceps militaris is not high, generally 0.1% or lower, and it is difficult to meet the requirement of 2% for commercial cordycepin. With the price of wild Cordyceps rising all the way, it has risen to about 200,000 yuan per kilogram, making wild Cordyceps harvested wildly. Therefore, from the perspective of resource protection, it is an urgent problem to be solved to find alternative strains of Cordyceps militaris ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/40C12R1/79
Inventor 李祝任秀秀肖洋梁宗琦韩燕峰周礼红
Owner GUIZHOU UNIV
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